9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers

A 9G DNAChip obtained by allowing the formation of a self-assembled monolayer (SAM) of oligonucleotides appended with nine consecutive guanines on the chip surface has been applied in the detection of biomarkers. Using a 9G DNAChip, biomarker in the concentration range of 4 pg/mL to 40 fg/mL can be...

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Detalles Bibliográficos
Autores principales: Nimse, Satish Balasaheb, Song, Keum-Soo, Kim, Junghoon, Sayyed, Danishmalik Rafiq, Kim, Taisun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634481/
https://www.ncbi.nlm.nih.gov/pubmed/23481635
http://dx.doi.org/10.3390/ijms14035723
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author Nimse, Satish Balasaheb
Song, Keum-Soo
Kim, Junghoon
Sayyed, Danishmalik Rafiq
Kim, Taisun
author_facet Nimse, Satish Balasaheb
Song, Keum-Soo
Kim, Junghoon
Sayyed, Danishmalik Rafiq
Kim, Taisun
author_sort Nimse, Satish Balasaheb
collection PubMed
description A 9G DNAChip obtained by allowing the formation of a self-assembled monolayer (SAM) of oligonucleotides appended with nine consecutive guanines on the chip surface has been applied in the detection of biomarkers. Using a 9G DNAChip, biomarker in the concentration range of 4 pg/mL to 40 fg/mL can be easily differentiated in the buffer matrix. Moreover, it is the first time that a biomarker with a concentration of 40 fg/mL has been detected in a mixture of proteins without use of any signal amplification technique.
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spelling pubmed-36344812013-05-02 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers Nimse, Satish Balasaheb Song, Keum-Soo Kim, Junghoon Sayyed, Danishmalik Rafiq Kim, Taisun Int J Mol Sci Article A 9G DNAChip obtained by allowing the formation of a self-assembled monolayer (SAM) of oligonucleotides appended with nine consecutive guanines on the chip surface has been applied in the detection of biomarkers. Using a 9G DNAChip, biomarker in the concentration range of 4 pg/mL to 40 fg/mL can be easily differentiated in the buffer matrix. Moreover, it is the first time that a biomarker with a concentration of 40 fg/mL has been detected in a mixture of proteins without use of any signal amplification technique. Molecular Diversity Preservation International (MDPI) 2013-03-12 /pmc/articles/PMC3634481/ /pubmed/23481635 http://dx.doi.org/10.3390/ijms14035723 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Nimse, Satish Balasaheb
Song, Keum-Soo
Kim, Junghoon
Sayyed, Danishmalik Rafiq
Kim, Taisun
9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers
title 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers
title_full 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers
title_fullStr 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers
title_full_unstemmed 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers
title_short 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers
title_sort 9g dnachip technology: self-assembled monolayer (sam) of ssdna for ultra-sensitive detection of biomarkers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634481/
https://www.ncbi.nlm.nih.gov/pubmed/23481635
http://dx.doi.org/10.3390/ijms14035723
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