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Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure

Translation elongation factor-1δ (TEF-1δ) has been identified as a novel cadmium-responsive proto-oncogene. However, it is still unclear whether TEF-1δ could be a potential biomarker of cadmium exposure. Rats were treated with CdCl(2) at different concentrations (high dose 1.225, mid-dose 0.612 and...

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Autores principales: Lu, Qian, Lei, Yi-Xiong, He, Chao-Cai, Lei, Zi-Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634498/
https://www.ncbi.nlm.nih.gov/pubmed/23459232
http://dx.doi.org/10.3390/ijms14035182
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author Lu, Qian
Lei, Yi-Xiong
He, Chao-Cai
Lei, Zi-Ning
author_facet Lu, Qian
Lei, Yi-Xiong
He, Chao-Cai
Lei, Zi-Ning
author_sort Lu, Qian
collection PubMed
description Translation elongation factor-1δ (TEF-1δ) has been identified as a novel cadmium-responsive proto-oncogene. However, it is still unclear whether TEF-1δ could be a potential biomarker of cadmium exposure. Rats were treated with CdCl(2) at different concentrations (high dose 1.225, mid-dose 0.612 and low dose 0.306 mg/kg body weight, respectively) for 14 weeks, and the cadmium levels, weight coefficients, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (SCR), 24-h urine protein (24hPro), urinary creatinine (Cr) and pathological features were determined. The TEF-1δ expression in white blood cells and multiple organs were examined by reverse transcription polymerase chain reaction (PCR) and were also confirmed with fluorescence quantitative PCR. A cadmium dose-dependent increase (p < 0.05) of cadmium levels in blood, urine, liver, kidney, heart and lung, and the weight coefficients was observed. The liver and renal function indictors including AST, ALT, SCR, BUN and 24hPro, were elevated in a cadmium dose-dependent manner (p < 0.05). Significant pathological changes in liver, kidney, heart and lung were indicated. The TEF-1δ expression was up-regulated in both blood and organs (p < 0.05). Moreover, the expression level of blood TEF-1δ was positively correlated to TEF-1δ expression level, cadmium level and toxicity in the organs (p < 0.01). This study indicates that blood TEF-1δ is a novel valuable biomarker for cadmium exposure and its organ toxicity.
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spelling pubmed-36344982013-05-02 Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure Lu, Qian Lei, Yi-Xiong He, Chao-Cai Lei, Zi-Ning Int J Mol Sci Article Translation elongation factor-1δ (TEF-1δ) has been identified as a novel cadmium-responsive proto-oncogene. However, it is still unclear whether TEF-1δ could be a potential biomarker of cadmium exposure. Rats were treated with CdCl(2) at different concentrations (high dose 1.225, mid-dose 0.612 and low dose 0.306 mg/kg body weight, respectively) for 14 weeks, and the cadmium levels, weight coefficients, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (SCR), 24-h urine protein (24hPro), urinary creatinine (Cr) and pathological features were determined. The TEF-1δ expression in white blood cells and multiple organs were examined by reverse transcription polymerase chain reaction (PCR) and were also confirmed with fluorescence quantitative PCR. A cadmium dose-dependent increase (p < 0.05) of cadmium levels in blood, urine, liver, kidney, heart and lung, and the weight coefficients was observed. The liver and renal function indictors including AST, ALT, SCR, BUN and 24hPro, were elevated in a cadmium dose-dependent manner (p < 0.05). Significant pathological changes in liver, kidney, heart and lung were indicated. The TEF-1δ expression was up-regulated in both blood and organs (p < 0.05). Moreover, the expression level of blood TEF-1δ was positively correlated to TEF-1δ expression level, cadmium level and toxicity in the organs (p < 0.01). This study indicates that blood TEF-1δ is a novel valuable biomarker for cadmium exposure and its organ toxicity. Molecular Diversity Preservation International (MDPI) 2013-03-04 /pmc/articles/PMC3634498/ /pubmed/23459232 http://dx.doi.org/10.3390/ijms14035182 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Lu, Qian
Lei, Yi-Xiong
He, Chao-Cai
Lei, Zi-Ning
Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure
title Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure
title_full Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure
title_fullStr Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure
title_full_unstemmed Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure
title_short Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure
title_sort blood translation elongation factor-1δ is a novel marker for cadmium exposure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634498/
https://www.ncbi.nlm.nih.gov/pubmed/23459232
http://dx.doi.org/10.3390/ijms14035182
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