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Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure
Translation elongation factor-1δ (TEF-1δ) has been identified as a novel cadmium-responsive proto-oncogene. However, it is still unclear whether TEF-1δ could be a potential biomarker of cadmium exposure. Rats were treated with CdCl(2) at different concentrations (high dose 1.225, mid-dose 0.612 and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634498/ https://www.ncbi.nlm.nih.gov/pubmed/23459232 http://dx.doi.org/10.3390/ijms14035182 |
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author | Lu, Qian Lei, Yi-Xiong He, Chao-Cai Lei, Zi-Ning |
author_facet | Lu, Qian Lei, Yi-Xiong He, Chao-Cai Lei, Zi-Ning |
author_sort | Lu, Qian |
collection | PubMed |
description | Translation elongation factor-1δ (TEF-1δ) has been identified as a novel cadmium-responsive proto-oncogene. However, it is still unclear whether TEF-1δ could be a potential biomarker of cadmium exposure. Rats were treated with CdCl(2) at different concentrations (high dose 1.225, mid-dose 0.612 and low dose 0.306 mg/kg body weight, respectively) for 14 weeks, and the cadmium levels, weight coefficients, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (SCR), 24-h urine protein (24hPro), urinary creatinine (Cr) and pathological features were determined. The TEF-1δ expression in white blood cells and multiple organs were examined by reverse transcription polymerase chain reaction (PCR) and were also confirmed with fluorescence quantitative PCR. A cadmium dose-dependent increase (p < 0.05) of cadmium levels in blood, urine, liver, kidney, heart and lung, and the weight coefficients was observed. The liver and renal function indictors including AST, ALT, SCR, BUN and 24hPro, were elevated in a cadmium dose-dependent manner (p < 0.05). Significant pathological changes in liver, kidney, heart and lung were indicated. The TEF-1δ expression was up-regulated in both blood and organs (p < 0.05). Moreover, the expression level of blood TEF-1δ was positively correlated to TEF-1δ expression level, cadmium level and toxicity in the organs (p < 0.01). This study indicates that blood TEF-1δ is a novel valuable biomarker for cadmium exposure and its organ toxicity. |
format | Online Article Text |
id | pubmed-3634498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36344982013-05-02 Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure Lu, Qian Lei, Yi-Xiong He, Chao-Cai Lei, Zi-Ning Int J Mol Sci Article Translation elongation factor-1δ (TEF-1δ) has been identified as a novel cadmium-responsive proto-oncogene. However, it is still unclear whether TEF-1δ could be a potential biomarker of cadmium exposure. Rats were treated with CdCl(2) at different concentrations (high dose 1.225, mid-dose 0.612 and low dose 0.306 mg/kg body weight, respectively) for 14 weeks, and the cadmium levels, weight coefficients, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (SCR), 24-h urine protein (24hPro), urinary creatinine (Cr) and pathological features were determined. The TEF-1δ expression in white blood cells and multiple organs were examined by reverse transcription polymerase chain reaction (PCR) and were also confirmed with fluorescence quantitative PCR. A cadmium dose-dependent increase (p < 0.05) of cadmium levels in blood, urine, liver, kidney, heart and lung, and the weight coefficients was observed. The liver and renal function indictors including AST, ALT, SCR, BUN and 24hPro, were elevated in a cadmium dose-dependent manner (p < 0.05). Significant pathological changes in liver, kidney, heart and lung were indicated. The TEF-1δ expression was up-regulated in both blood and organs (p < 0.05). Moreover, the expression level of blood TEF-1δ was positively correlated to TEF-1δ expression level, cadmium level and toxicity in the organs (p < 0.01). This study indicates that blood TEF-1δ is a novel valuable biomarker for cadmium exposure and its organ toxicity. Molecular Diversity Preservation International (MDPI) 2013-03-04 /pmc/articles/PMC3634498/ /pubmed/23459232 http://dx.doi.org/10.3390/ijms14035182 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Lu, Qian Lei, Yi-Xiong He, Chao-Cai Lei, Zi-Ning Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure |
title | Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure |
title_full | Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure |
title_fullStr | Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure |
title_full_unstemmed | Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure |
title_short | Blood Translation Elongation Factor-1δ Is a Novel Marker for Cadmium Exposure |
title_sort | blood translation elongation factor-1δ is a novel marker for cadmium exposure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634498/ https://www.ncbi.nlm.nih.gov/pubmed/23459232 http://dx.doi.org/10.3390/ijms14035182 |
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