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Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea

Hearing loss is frequent in intensive care patients and can be due to several causes. However, sepsis has not been examined as a possible cause. The aim of this study is to assess the influence of experimental sepsis on hearing thresholds and to evaluate pathological changes in the cochlea. The ceca...

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Autores principales: Schmutzhard, Joachim, Glueckert, Rudolf, Pritz, Christian, Blumer, Michael J. F., Bitsche, Mario, Lackner, Peter, Fille, Manfred, Riechelmann, Herbert, Harkamp, Matthias, Sitthisak, Thongrong, Schrott-Fischer, Annelies
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Limited 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634657/
https://www.ncbi.nlm.nih.gov/pubmed/23471916
http://dx.doi.org/10.1242/dmm.011205
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author Schmutzhard, Joachim
Glueckert, Rudolf
Pritz, Christian
Blumer, Michael J. F.
Bitsche, Mario
Lackner, Peter
Fille, Manfred
Riechelmann, Herbert
Harkamp, Matthias
Sitthisak, Thongrong
Schrott-Fischer, Annelies
author_facet Schmutzhard, Joachim
Glueckert, Rudolf
Pritz, Christian
Blumer, Michael J. F.
Bitsche, Mario
Lackner, Peter
Fille, Manfred
Riechelmann, Herbert
Harkamp, Matthias
Sitthisak, Thongrong
Schrott-Fischer, Annelies
author_sort Schmutzhard, Joachim
collection PubMed
description Hearing loss is frequent in intensive care patients and can be due to several causes. However, sepsis has not been examined as a possible cause. The aim of this study is to assess the influence of experimental sepsis on hearing thresholds and to evaluate pathological changes in the cochlea. The cecal ligation puncture technique was used to induce sepsis in 18 mice. Results were compared with those from 13 sham-operated and 13 untreated control mice. The hearing thresholds of the animals were evaluated with auditory evoked brainstem responses prior to the induction of sepsis and again at the peak of the disease. Immediately after the second measurement, the mice were sacrificed and the inner ears harvested and prepared for further evaluation. The cochleae were examined with light microscopy, electron microscopy and immunohistochemistry for Bax, cleaved caspase-3 and Bcl-2. The mice with sepsis showed a significant hearing loss but not the control groups. Induction of apoptosis could be shown in the supporting cells of the organ of Corti. Furthermore, excitotoxicity could be shown at the basal pole of the inner hair cells. In this murine model, sepsis leads to significant hearing impairment. The physiological alteration could be linked to apoptosis in the supporting cells of the organ of Corti and to a disturbance of the synapses of the inner hair cells.
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spelling pubmed-36346572013-06-19 Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea Schmutzhard, Joachim Glueckert, Rudolf Pritz, Christian Blumer, Michael J. F. Bitsche, Mario Lackner, Peter Fille, Manfred Riechelmann, Herbert Harkamp, Matthias Sitthisak, Thongrong Schrott-Fischer, Annelies Dis Model Mech Research Article Hearing loss is frequent in intensive care patients and can be due to several causes. However, sepsis has not been examined as a possible cause. The aim of this study is to assess the influence of experimental sepsis on hearing thresholds and to evaluate pathological changes in the cochlea. The cecal ligation puncture technique was used to induce sepsis in 18 mice. Results were compared with those from 13 sham-operated and 13 untreated control mice. The hearing thresholds of the animals were evaluated with auditory evoked brainstem responses prior to the induction of sepsis and again at the peak of the disease. Immediately after the second measurement, the mice were sacrificed and the inner ears harvested and prepared for further evaluation. The cochleae were examined with light microscopy, electron microscopy and immunohistochemistry for Bax, cleaved caspase-3 and Bcl-2. The mice with sepsis showed a significant hearing loss but not the control groups. Induction of apoptosis could be shown in the supporting cells of the organ of Corti. Furthermore, excitotoxicity could be shown at the basal pole of the inner hair cells. In this murine model, sepsis leads to significant hearing impairment. The physiological alteration could be linked to apoptosis in the supporting cells of the organ of Corti and to a disturbance of the synapses of the inner hair cells. The Company of Biologists Limited 2013-05 2013-02-14 /pmc/articles/PMC3634657/ /pubmed/23471916 http://dx.doi.org/10.1242/dmm.011205 Text en © 2013. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.
spellingShingle Research Article
Schmutzhard, Joachim
Glueckert, Rudolf
Pritz, Christian
Blumer, Michael J. F.
Bitsche, Mario
Lackner, Peter
Fille, Manfred
Riechelmann, Herbert
Harkamp, Matthias
Sitthisak, Thongrong
Schrott-Fischer, Annelies
Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
title Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
title_full Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
title_fullStr Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
title_full_unstemmed Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
title_short Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
title_sort sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634657/
https://www.ncbi.nlm.nih.gov/pubmed/23471916
http://dx.doi.org/10.1242/dmm.011205
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