Cargando…
Tools to Detect Influenza Virus
In 2009, pandemic influenza A (H1N1) virus (H1N1 09) started to spread quickly in many countries. It causes respiratory infection with signs and symptoms of common infectious agents. Thus, clinicians sometimes may miss the H1N1 patient. Clinical laboratory tests are important for the diagnosis of th...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Yonsei University College of Medicine
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3635619/ https://www.ncbi.nlm.nih.gov/pubmed/23549796 http://dx.doi.org/10.3349/ymj.2013.54.3.560 |
_version_ | 1782267207492829184 |
---|---|
author | Kim, Dae-Ki Poudel, Barun |
author_facet | Kim, Dae-Ki Poudel, Barun |
author_sort | Kim, Dae-Ki |
collection | PubMed |
description | In 2009, pandemic influenza A (H1N1) virus (H1N1 09) started to spread quickly in many countries. It causes respiratory infection with signs and symptoms of common infectious agents. Thus, clinicians sometimes may miss the H1N1 patient. Clinical laboratory tests are important for the diagnosis of the H1N1 infection. There are several tests available, however, the rapid test and direct fluorescence antigen test are unable to rule out the influenza virus infection and viral culture test is time consuming. Therefore, nucleic acid amplification techniques based on reverse transcription polymerase chain reaction assays are regarded as a specific diagnosis to confirm the influenza virus infection. Although the nucleic acid-based techniques are highly sensitive and specific, the high mutation rate of the influenza RNA-dependent RNA polymerase could limit the utility of the techniques. In addition, their use depends on the availability, cost and throughput of the diagnostic techniques. To overcome these drawbacks, evaluation and development of the techniques should be continued. This review provides an overview of various techniques for specific diagnosis of influenza infection. |
format | Online Article Text |
id | pubmed-3635619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Yonsei University College of Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-36356192013-05-02 Tools to Detect Influenza Virus Kim, Dae-Ki Poudel, Barun Yonsei Med J Review Article In 2009, pandemic influenza A (H1N1) virus (H1N1 09) started to spread quickly in many countries. It causes respiratory infection with signs and symptoms of common infectious agents. Thus, clinicians sometimes may miss the H1N1 patient. Clinical laboratory tests are important for the diagnosis of the H1N1 infection. There are several tests available, however, the rapid test and direct fluorescence antigen test are unable to rule out the influenza virus infection and viral culture test is time consuming. Therefore, nucleic acid amplification techniques based on reverse transcription polymerase chain reaction assays are regarded as a specific diagnosis to confirm the influenza virus infection. Although the nucleic acid-based techniques are highly sensitive and specific, the high mutation rate of the influenza RNA-dependent RNA polymerase could limit the utility of the techniques. In addition, their use depends on the availability, cost and throughput of the diagnostic techniques. To overcome these drawbacks, evaluation and development of the techniques should be continued. This review provides an overview of various techniques for specific diagnosis of influenza infection. Yonsei University College of Medicine 2013-05-01 2013-03-19 /pmc/articles/PMC3635619/ /pubmed/23549796 http://dx.doi.org/10.3349/ymj.2013.54.3.560 Text en © Copyright: Yonsei University College of Medicine 2013 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Kim, Dae-Ki Poudel, Barun Tools to Detect Influenza Virus |
title | Tools to Detect Influenza Virus |
title_full | Tools to Detect Influenza Virus |
title_fullStr | Tools to Detect Influenza Virus |
title_full_unstemmed | Tools to Detect Influenza Virus |
title_short | Tools to Detect Influenza Virus |
title_sort | tools to detect influenza virus |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3635619/ https://www.ncbi.nlm.nih.gov/pubmed/23549796 http://dx.doi.org/10.3349/ymj.2013.54.3.560 |
work_keys_str_mv | AT kimdaeki toolstodetectinfluenzavirus AT poudelbarun toolstodetectinfluenzavirus |