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The Effects of Schwann and Bone Marrow Stromal Stem Cells on Sciatic Nerve Injury in Rat: A Comparison of Functional Recovery
OBJECTIVE: Transplantation of bone marrow stromal cells (BMSCs) or Schwann cells (SCs) can facilitate axonal regeneration in peripheral nerve injuries. The aim of this study was to compare the effects of transplantation of BMSCs and SCs on functional recovery after injury to the sciatic nerve in the...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3635819/ https://www.ncbi.nlm.nih.gov/pubmed/23626936 |
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author | Zarbakhsh, Sam Bakhtiyari, Mehrdad Faghihi, Abolfazl Joghataei, Mohammad Taghi Mehdizadeh, Mehdi Khoei, Samideh Mansouri, Korosh Yousefi, Behpour Pirhajati, Vahid Moradi, Fatemeh Shirmohammadi, Maryam |
author_facet | Zarbakhsh, Sam Bakhtiyari, Mehrdad Faghihi, Abolfazl Joghataei, Mohammad Taghi Mehdizadeh, Mehdi Khoei, Samideh Mansouri, Korosh Yousefi, Behpour Pirhajati, Vahid Moradi, Fatemeh Shirmohammadi, Maryam |
author_sort | Zarbakhsh, Sam |
collection | PubMed |
description | OBJECTIVE: Transplantation of bone marrow stromal cells (BMSCs) or Schwann cells (SCs) can facilitate axonal regeneration in peripheral nerve injuries. The aim of this study was to compare the effects of transplantation of BMSCs and SCs on functional recovery after injury to the sciatic nerve in the rat. MATERIALS AND METHODS: In this experimental research, adult male Wistar rats (n=24, 250-300 g) were used, BMSCs and SCs were cultured, and SCs were confirmed with anti S100 antibody. Rats were randomly divided into 3 groups (n=8 in each group): 1; control group: silicon tube filled with fibrin gel without the cells, 2; BMSCs group: silicon tube filled with fibrin gel seeded with BMSCs and 3; SCs group: silicon tube filled with fibrin gel seeded with SCs. The left sciatic nerve was exposed, a 10 mm segment removed, and a silicone tube interposed into this nerve gap. BMSCs and SCs were separately transplanted into the gap in the two experimental groups and were labeled with anti BrdU and DiI respectively. After 12 weeks electrophysiological and functional assessments were performed and analyzed by one-way analysis of variance (ANOVA). RESULTS: Electrophysiological and functional assessments showed a significant difference between the experimental groups compared with the control group. Electrophysiological measures were significantly better in the SCs transplantation group compared with the BMSCs treatment group (p <0.05). Functional assessments showed no statistically significant difference between the BMSCs and SCs groups (p <0.05). CONCLUSION: Although both BMSCs and SCs have the potential to produce functional recovery after injury to the sciatic nerve in rats, electrophysiological evaluation confirms that the improvement after SCs transplantation is greater than that after BMSCs transplantation. |
format | Online Article Text |
id | pubmed-3635819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-36358192013-04-26 The Effects of Schwann and Bone Marrow Stromal Stem Cells on Sciatic Nerve Injury in Rat: A Comparison of Functional Recovery Zarbakhsh, Sam Bakhtiyari, Mehrdad Faghihi, Abolfazl Joghataei, Mohammad Taghi Mehdizadeh, Mehdi Khoei, Samideh Mansouri, Korosh Yousefi, Behpour Pirhajati, Vahid Moradi, Fatemeh Shirmohammadi, Maryam Cell J Research Article OBJECTIVE: Transplantation of bone marrow stromal cells (BMSCs) or Schwann cells (SCs) can facilitate axonal regeneration in peripheral nerve injuries. The aim of this study was to compare the effects of transplantation of BMSCs and SCs on functional recovery after injury to the sciatic nerve in the rat. MATERIALS AND METHODS: In this experimental research, adult male Wistar rats (n=24, 250-300 g) were used, BMSCs and SCs were cultured, and SCs were confirmed with anti S100 antibody. Rats were randomly divided into 3 groups (n=8 in each group): 1; control group: silicon tube filled with fibrin gel without the cells, 2; BMSCs group: silicon tube filled with fibrin gel seeded with BMSCs and 3; SCs group: silicon tube filled with fibrin gel seeded with SCs. The left sciatic nerve was exposed, a 10 mm segment removed, and a silicone tube interposed into this nerve gap. BMSCs and SCs were separately transplanted into the gap in the two experimental groups and were labeled with anti BrdU and DiI respectively. After 12 weeks electrophysiological and functional assessments were performed and analyzed by one-way analysis of variance (ANOVA). RESULTS: Electrophysiological and functional assessments showed a significant difference between the experimental groups compared with the control group. Electrophysiological measures were significantly better in the SCs transplantation group compared with the BMSCs treatment group (p <0.05). Functional assessments showed no statistically significant difference between the BMSCs and SCs groups (p <0.05). CONCLUSION: Although both BMSCs and SCs have the potential to produce functional recovery after injury to the sciatic nerve in rats, electrophysiological evaluation confirms that the improvement after SCs transplantation is greater than that after BMSCs transplantation. Royan Institute 2012 2012-06-13 /pmc/articles/PMC3635819/ /pubmed/23626936 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zarbakhsh, Sam Bakhtiyari, Mehrdad Faghihi, Abolfazl Joghataei, Mohammad Taghi Mehdizadeh, Mehdi Khoei, Samideh Mansouri, Korosh Yousefi, Behpour Pirhajati, Vahid Moradi, Fatemeh Shirmohammadi, Maryam The Effects of Schwann and Bone Marrow Stromal Stem Cells on Sciatic Nerve Injury in Rat: A Comparison of Functional Recovery |
title | The Effects of Schwann and Bone Marrow Stromal Stem Cells on Sciatic Nerve Injury in Rat: A Comparison of Functional Recovery |
title_full | The Effects of Schwann and Bone Marrow Stromal Stem Cells on Sciatic Nerve Injury in Rat: A Comparison of Functional Recovery |
title_fullStr | The Effects of Schwann and Bone Marrow Stromal Stem Cells on Sciatic Nerve Injury in Rat: A Comparison of Functional Recovery |
title_full_unstemmed | The Effects of Schwann and Bone Marrow Stromal Stem Cells on Sciatic Nerve Injury in Rat: A Comparison of Functional Recovery |
title_short | The Effects of Schwann and Bone Marrow Stromal Stem Cells on Sciatic Nerve Injury in Rat: A Comparison of Functional Recovery |
title_sort | effects of schwann and bone marrow stromal stem cells on sciatic nerve injury in rat: a comparison of functional recovery |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3635819/ https://www.ncbi.nlm.nih.gov/pubmed/23626936 |
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