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Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances

BACKGROUND: Cigarette smoking is a cause of a variety of serious diseases, and to understand the toxicological impact of tobacco smoke in vitro, whole smoke exposure systems can be used. One of the main challenges of the different whole smoke exposure systems that are commercially available is that...

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Autores principales: Adamson, Jason, Thorne, David, Dalrymple, Annette, Dillon, Debbie, Meredith, Clive
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3635897/
https://www.ncbi.nlm.nih.gov/pubmed/23497606
http://dx.doi.org/10.1186/1752-153X-7-50
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author Adamson, Jason
Thorne, David
Dalrymple, Annette
Dillon, Debbie
Meredith, Clive
author_facet Adamson, Jason
Thorne, David
Dalrymple, Annette
Dillon, Debbie
Meredith, Clive
author_sort Adamson, Jason
collection PubMed
description BACKGROUND: Cigarette smoking is a cause of a variety of serious diseases, and to understand the toxicological impact of tobacco smoke in vitro, whole smoke exposure systems can be used. One of the main challenges of the different whole smoke exposure systems that are commercially available is that they dilute and deliver smoke in different ways, limiting/restricting the cross-comparison of biological responses. This is where dosimetry – dose quantification – can play a key role in data comparison. Quartz crystal microbalance (QCM) technology has been put forward as one such tool to quantify smoke particle deposition in vitro, in real-time. RESULTS: Using four identical QCMs, installed into the Vitrocell® mammalian 6/4 CF Stainless exposure module, we were able to quantify deposited smoke particle deposition, generated and diluted by a Vitrocell® VC 10 Smoking Robot. At diluting airflows 0.5-4.0 L/min and vacuum flow rate 5 ml/min/well through the exposure module, mean particle deposition was in the range 8.65 ± 1.51 μg/cm(2)-0.72 ± 0.13 μg/cm(2). Additionally, the effect of varying vacuum flow rate on particle deposition was assessed from 5 ml/min/well - 100 ml/min/well. Mean deposited mass for all four airflows tested per vacuum decreased as vacuum rate was increased: mean deposition was 3.79, 2.75, 1.56 and 1.09 μg/cm(2) at vacuum rates of 5, 10, 50 and 100 ml/min/well respectively. CONCLUSIONS: QCMs within the Vitrocell® exposure module have demonstrated applicability at defining particle dose ranges at various experimental conditions. This tool will prove useful for users of the Vitrocell® system for dose–response determination and QC purposes.
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spelling pubmed-36358972013-04-26 Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances Adamson, Jason Thorne, David Dalrymple, Annette Dillon, Debbie Meredith, Clive Chem Cent J Research Article BACKGROUND: Cigarette smoking is a cause of a variety of serious diseases, and to understand the toxicological impact of tobacco smoke in vitro, whole smoke exposure systems can be used. One of the main challenges of the different whole smoke exposure systems that are commercially available is that they dilute and deliver smoke in different ways, limiting/restricting the cross-comparison of biological responses. This is where dosimetry – dose quantification – can play a key role in data comparison. Quartz crystal microbalance (QCM) technology has been put forward as one such tool to quantify smoke particle deposition in vitro, in real-time. RESULTS: Using four identical QCMs, installed into the Vitrocell® mammalian 6/4 CF Stainless exposure module, we were able to quantify deposited smoke particle deposition, generated and diluted by a Vitrocell® VC 10 Smoking Robot. At diluting airflows 0.5-4.0 L/min and vacuum flow rate 5 ml/min/well through the exposure module, mean particle deposition was in the range 8.65 ± 1.51 μg/cm(2)-0.72 ± 0.13 μg/cm(2). Additionally, the effect of varying vacuum flow rate on particle deposition was assessed from 5 ml/min/well - 100 ml/min/well. Mean deposited mass for all four airflows tested per vacuum decreased as vacuum rate was increased: mean deposition was 3.79, 2.75, 1.56 and 1.09 μg/cm(2) at vacuum rates of 5, 10, 50 and 100 ml/min/well respectively. CONCLUSIONS: QCMs within the Vitrocell® exposure module have demonstrated applicability at defining particle dose ranges at various experimental conditions. This tool will prove useful for users of the Vitrocell® system for dose–response determination and QC purposes. BioMed Central 2013-03-12 /pmc/articles/PMC3635897/ /pubmed/23497606 http://dx.doi.org/10.1186/1752-153X-7-50 Text en Copyright © 2013 Adamson et al.; licensee Chemistry Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Adamson, Jason
Thorne, David
Dalrymple, Annette
Dillon, Debbie
Meredith, Clive
Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances
title Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances
title_full Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances
title_fullStr Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances
title_full_unstemmed Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances
title_short Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances
title_sort assessment of cigarette smoke particle deposition within the vitrocell® exposure module using quartz crystal microbalances
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3635897/
https://www.ncbi.nlm.nih.gov/pubmed/23497606
http://dx.doi.org/10.1186/1752-153X-7-50
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