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Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence
Pathogen access to host nutrients in infected tissues is fundamental for pathogen growth and virulence, disease progression, and infection control. However, our understanding of this crucial process is still rather limited because of experimental and conceptual challenges. Here, we used proteomics,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636032/ https://www.ncbi.nlm.nih.gov/pubmed/23633950 http://dx.doi.org/10.1371/journal.ppat.1003301 |
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author | Steeb, Benjamin Claudi, Beatrice Burton, Neil A. Tienz, Petra Schmidt, Alexander Farhan, Hesso Mazé, Alain Bumann, Dirk |
author_facet | Steeb, Benjamin Claudi, Beatrice Burton, Neil A. Tienz, Petra Schmidt, Alexander Farhan, Hesso Mazé, Alain Bumann, Dirk |
author_sort | Steeb, Benjamin |
collection | PubMed |
description | Pathogen access to host nutrients in infected tissues is fundamental for pathogen growth and virulence, disease progression, and infection control. However, our understanding of this crucial process is still rather limited because of experimental and conceptual challenges. Here, we used proteomics, microbial genetics, competitive infections, and computational approaches to obtain a comprehensive overview of Salmonella nutrition and growth in a mouse typhoid fever model. The data revealed that Salmonella accessed an unexpectedly diverse set of at least 31 different host nutrients in infected tissues but the individual nutrients were available in only scarce amounts. Salmonella adapted to this situation by expressing versatile catabolic pathways to simultaneously exploit multiple host nutrients. A genome-scale computational model of Salmonella in vivo metabolism based on these data was fully consistent with independent large-scale experimental data on Salmonella enzyme quantities, and correctly predicted 92% of 738 reported experimental mutant virulence phenotypes, suggesting that our analysis provided a comprehensive overview of host nutrient supply, Salmonella metabolism, and Salmonella growth during infection. Comparison of metabolic networks of other pathogens suggested that complex host/pathogen nutritional interfaces are a common feature underlying many infectious diseases. |
format | Online Article Text |
id | pubmed-3636032 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36360322013-04-30 Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence Steeb, Benjamin Claudi, Beatrice Burton, Neil A. Tienz, Petra Schmidt, Alexander Farhan, Hesso Mazé, Alain Bumann, Dirk PLoS Pathog Research Article Pathogen access to host nutrients in infected tissues is fundamental for pathogen growth and virulence, disease progression, and infection control. However, our understanding of this crucial process is still rather limited because of experimental and conceptual challenges. Here, we used proteomics, microbial genetics, competitive infections, and computational approaches to obtain a comprehensive overview of Salmonella nutrition and growth in a mouse typhoid fever model. The data revealed that Salmonella accessed an unexpectedly diverse set of at least 31 different host nutrients in infected tissues but the individual nutrients were available in only scarce amounts. Salmonella adapted to this situation by expressing versatile catabolic pathways to simultaneously exploit multiple host nutrients. A genome-scale computational model of Salmonella in vivo metabolism based on these data was fully consistent with independent large-scale experimental data on Salmonella enzyme quantities, and correctly predicted 92% of 738 reported experimental mutant virulence phenotypes, suggesting that our analysis provided a comprehensive overview of host nutrient supply, Salmonella metabolism, and Salmonella growth during infection. Comparison of metabolic networks of other pathogens suggested that complex host/pathogen nutritional interfaces are a common feature underlying many infectious diseases. Public Library of Science 2013-04-25 /pmc/articles/PMC3636032/ /pubmed/23633950 http://dx.doi.org/10.1371/journal.ppat.1003301 Text en © 2013 Steeb et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Steeb, Benjamin Claudi, Beatrice Burton, Neil A. Tienz, Petra Schmidt, Alexander Farhan, Hesso Mazé, Alain Bumann, Dirk Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence |
title | Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence |
title_full | Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence |
title_fullStr | Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence |
title_full_unstemmed | Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence |
title_short | Parallel Exploitation of Diverse Host Nutrients Enhances Salmonella Virulence |
title_sort | parallel exploitation of diverse host nutrients enhances salmonella virulence |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636032/ https://www.ncbi.nlm.nih.gov/pubmed/23633950 http://dx.doi.org/10.1371/journal.ppat.1003301 |
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