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Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)
BACKGROUND: Recent studies have demonstrated that cellular energy is a key factor switching on ripening and senescence of fruit. However, the factors that influence fruit energy status remain largely unknown. RESULTS: HPLC profiling showed that ATP abundance increased significantly in developing pre...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636124/ https://www.ncbi.nlm.nih.gov/pubmed/23547657 http://dx.doi.org/10.1186/1471-2229-13-55 |
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author | Wang, Hui Qian, Zhengjiang Ma, Sanmei Zhou, Yuchuan Patrick, John W Duan, Xuewu Jiang, Yueming Qu, Hongxia |
author_facet | Wang, Hui Qian, Zhengjiang Ma, Sanmei Zhou, Yuchuan Patrick, John W Duan, Xuewu Jiang, Yueming Qu, Hongxia |
author_sort | Wang, Hui |
collection | PubMed |
description | BACKGROUND: Recent studies have demonstrated that cellular energy is a key factor switching on ripening and senescence of fruit. However, the factors that influence fruit energy status remain largely unknown. RESULTS: HPLC profiling showed that ATP abundance increased significantly in developing preharvest litchi fruit and was strongly correlated with fruit fresh weight. In contrast, ATP levels declined significantly during postharvest fruit senescence and were correlated with the decrease in the proportion of edible fruit. The five gene transcripts isolated from the litchi fruit pericarp were highly expressed in vegetative tissues and peaked at 70 days after flowering (DAF) consistent with fruit ADP concentrations, except for uncoupling mitochondrial protein 1 (UCP1), which was predominantly expressed in the root, and ATP synthase beta subunit (AtpB), which was up-regulated significantly before harvest and peaked 2 days after storage. These results indicated that the color-breaker stage at 70 DAF and 2 days after storage may be key turning points in fruit energy metabolism. Transcript abundance of alternative oxidase 1 (AOX1) increased after 2 days of storage to significantly higher levels than those of LcAtpB, and was down-regulated significantly by exogenous ATP. ATP supplementation had no significant effect on transcript abundance of ADP/ATP carrier 1 (AAC1) and slowed the changes in sucrose non-fermenting-1-related kinase 2 (SnRK2) expression, but maintained ATP and energy charge levels, which were correlated with delayed senescence. CONCLUSIONS: Our results suggest that senescence of litchi fruit is closely related with energy. A surge of LcAtpB expression marked the beginning of fruit senescence. The findings may provide a new strategy to extend fruit shelf life by regulating its energy level. |
format | Online Article Text |
id | pubmed-3636124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36361242013-04-26 Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.) Wang, Hui Qian, Zhengjiang Ma, Sanmei Zhou, Yuchuan Patrick, John W Duan, Xuewu Jiang, Yueming Qu, Hongxia BMC Plant Biol Research Article BACKGROUND: Recent studies have demonstrated that cellular energy is a key factor switching on ripening and senescence of fruit. However, the factors that influence fruit energy status remain largely unknown. RESULTS: HPLC profiling showed that ATP abundance increased significantly in developing preharvest litchi fruit and was strongly correlated with fruit fresh weight. In contrast, ATP levels declined significantly during postharvest fruit senescence and were correlated with the decrease in the proportion of edible fruit. The five gene transcripts isolated from the litchi fruit pericarp were highly expressed in vegetative tissues and peaked at 70 days after flowering (DAF) consistent with fruit ADP concentrations, except for uncoupling mitochondrial protein 1 (UCP1), which was predominantly expressed in the root, and ATP synthase beta subunit (AtpB), which was up-regulated significantly before harvest and peaked 2 days after storage. These results indicated that the color-breaker stage at 70 DAF and 2 days after storage may be key turning points in fruit energy metabolism. Transcript abundance of alternative oxidase 1 (AOX1) increased after 2 days of storage to significantly higher levels than those of LcAtpB, and was down-regulated significantly by exogenous ATP. ATP supplementation had no significant effect on transcript abundance of ADP/ATP carrier 1 (AAC1) and slowed the changes in sucrose non-fermenting-1-related kinase 2 (SnRK2) expression, but maintained ATP and energy charge levels, which were correlated with delayed senescence. CONCLUSIONS: Our results suggest that senescence of litchi fruit is closely related with energy. A surge of LcAtpB expression marked the beginning of fruit senescence. The findings may provide a new strategy to extend fruit shelf life by regulating its energy level. BioMed Central 2013-04-02 /pmc/articles/PMC3636124/ /pubmed/23547657 http://dx.doi.org/10.1186/1471-2229-13-55 Text en Copyright © 2013 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Hui Qian, Zhengjiang Ma, Sanmei Zhou, Yuchuan Patrick, John W Duan, Xuewu Jiang, Yueming Qu, Hongxia Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.) |
title | Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.) |
title_full | Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.) |
title_fullStr | Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.) |
title_full_unstemmed | Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.) |
title_short | Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.) |
title_sort | energy status of ripening and postharvest senescent fruit of litchi (litchi chinensis sonn.) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636124/ https://www.ncbi.nlm.nih.gov/pubmed/23547657 http://dx.doi.org/10.1186/1471-2229-13-55 |
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