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Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study

BACKGROUND: The human gut microbiota has profound influence on host metabolism and immunity. This study characterized the fecal microbiota in patients with nonalcoholic steatohepatitis (NASH). The relationship between microbiota changes and changes in hepatic steatosis was also studied. METHODS: Fec...

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Autores principales: Wong, Vincent Wai-Sun, Tse, Chi-Hang, Lam, Tommy Tsan-Yuk, Wong, Grace Lai-Hung, Chim, Angel Mei-Ling, Chu, Winnie Chiu-Wing, Yeung, David Ka-Wai, Law, Patrick Tik-Wan, Kwan, Hoi-Shan, Yu, Jun, Sung, Joseph Jao-Yiu, Chan, Henry Lik-Yuen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636208/
https://www.ncbi.nlm.nih.gov/pubmed/23638162
http://dx.doi.org/10.1371/journal.pone.0062885
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author Wong, Vincent Wai-Sun
Tse, Chi-Hang
Lam, Tommy Tsan-Yuk
Wong, Grace Lai-Hung
Chim, Angel Mei-Ling
Chu, Winnie Chiu-Wing
Yeung, David Ka-Wai
Law, Patrick Tik-Wan
Kwan, Hoi-Shan
Yu, Jun
Sung, Joseph Jao-Yiu
Chan, Henry Lik-Yuen
author_facet Wong, Vincent Wai-Sun
Tse, Chi-Hang
Lam, Tommy Tsan-Yuk
Wong, Grace Lai-Hung
Chim, Angel Mei-Ling
Chu, Winnie Chiu-Wing
Yeung, David Ka-Wai
Law, Patrick Tik-Wan
Kwan, Hoi-Shan
Yu, Jun
Sung, Joseph Jao-Yiu
Chan, Henry Lik-Yuen
author_sort Wong, Vincent Wai-Sun
collection PubMed
description BACKGROUND: The human gut microbiota has profound influence on host metabolism and immunity. This study characterized the fecal microbiota in patients with nonalcoholic steatohepatitis (NASH). The relationship between microbiota changes and changes in hepatic steatosis was also studied. METHODS: Fecal microbiota of histology-proven NASH patients and healthy controls was analyzed by 16S ribosomal RNA pyrosequencing. NASH patients were from a previously reported randomized trial on probiotic treatment. Proton-magnetic resonance spectroscopy was performed to monitor changes in intrahepatic triglyceride content (IHTG). RESULTS: A total of 420,344 16S sequences with acceptable quality were obtained from 16 NASH patients and 22 controls. NASH patients had lower fecal abundance of Faecalibacterium and Anaerosporobacter but higher abundance of Parabacteroides and Allisonella. Partial least-square discriminant analysis yielded a model of 10 genera that discriminated NASH patients from controls. At month 6, 6 of 7 patients in the probiotic group and 4 of 9 patients in the usual care group had improvement in IHTG (P = 0.15). Improvement in IHTG was associated with a reduction in the abundance of Firmicutes (R(2) = 0.4820, P = 0.0028) and increase in Bacteroidetes (R(2) = 0.4366, P = 0.0053). This was accompanied by corresponding changes at the class, order and genus levels. In contrast, bacterial biodiversity did not differ between NASH patients and controls, and did not change with probiotic treatment. CONCLUSIONS: NASH patients have fecal dysbiosis, and changes in microbiota correlate with improvement in hepatic steatosis. Further studies are required to investigate the mechanism underlying the interaction between gut microbes and the liver.
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spelling pubmed-36362082013-05-01 Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study Wong, Vincent Wai-Sun Tse, Chi-Hang Lam, Tommy Tsan-Yuk Wong, Grace Lai-Hung Chim, Angel Mei-Ling Chu, Winnie Chiu-Wing Yeung, David Ka-Wai Law, Patrick Tik-Wan Kwan, Hoi-Shan Yu, Jun Sung, Joseph Jao-Yiu Chan, Henry Lik-Yuen PLoS One Research Article BACKGROUND: The human gut microbiota has profound influence on host metabolism and immunity. This study characterized the fecal microbiota in patients with nonalcoholic steatohepatitis (NASH). The relationship between microbiota changes and changes in hepatic steatosis was also studied. METHODS: Fecal microbiota of histology-proven NASH patients and healthy controls was analyzed by 16S ribosomal RNA pyrosequencing. NASH patients were from a previously reported randomized trial on probiotic treatment. Proton-magnetic resonance spectroscopy was performed to monitor changes in intrahepatic triglyceride content (IHTG). RESULTS: A total of 420,344 16S sequences with acceptable quality were obtained from 16 NASH patients and 22 controls. NASH patients had lower fecal abundance of Faecalibacterium and Anaerosporobacter but higher abundance of Parabacteroides and Allisonella. Partial least-square discriminant analysis yielded a model of 10 genera that discriminated NASH patients from controls. At month 6, 6 of 7 patients in the probiotic group and 4 of 9 patients in the usual care group had improvement in IHTG (P = 0.15). Improvement in IHTG was associated with a reduction in the abundance of Firmicutes (R(2) = 0.4820, P = 0.0028) and increase in Bacteroidetes (R(2) = 0.4366, P = 0.0053). This was accompanied by corresponding changes at the class, order and genus levels. In contrast, bacterial biodiversity did not differ between NASH patients and controls, and did not change with probiotic treatment. CONCLUSIONS: NASH patients have fecal dysbiosis, and changes in microbiota correlate with improvement in hepatic steatosis. Further studies are required to investigate the mechanism underlying the interaction between gut microbes and the liver. Public Library of Science 2013-04-25 /pmc/articles/PMC3636208/ /pubmed/23638162 http://dx.doi.org/10.1371/journal.pone.0062885 Text en © 2013 Wong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wong, Vincent Wai-Sun
Tse, Chi-Hang
Lam, Tommy Tsan-Yuk
Wong, Grace Lai-Hung
Chim, Angel Mei-Ling
Chu, Winnie Chiu-Wing
Yeung, David Ka-Wai
Law, Patrick Tik-Wan
Kwan, Hoi-Shan
Yu, Jun
Sung, Joseph Jao-Yiu
Chan, Henry Lik-Yuen
Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study
title Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study
title_full Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study
title_fullStr Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study
title_full_unstemmed Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study
title_short Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study
title_sort molecular characterization of the fecal microbiota in patients with nonalcoholic steatohepatitis – a longitudinal study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636208/
https://www.ncbi.nlm.nih.gov/pubmed/23638162
http://dx.doi.org/10.1371/journal.pone.0062885
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