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Changes in retinal aquaporin-9 (AQP9) expression in glaucoma

The eye contains numerous water channel proteins and the roles of AQPs (aquaporins) in the retina are blurred, especially under disease conditions. The purpose of this study was to investigate the expression of AQP9 gene and proteins affected by elevated IOP (intraocular pressure) in a rat model of...

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Autores principales: Yang, Ming-Hui, Dibas, Adnan, Tyan, Yu-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636522/
https://www.ncbi.nlm.nih.gov/pubmed/23464865
http://dx.doi.org/10.1042/BSR20130005
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author Yang, Ming-Hui
Dibas, Adnan
Tyan, Yu-Chang
author_facet Yang, Ming-Hui
Dibas, Adnan
Tyan, Yu-Chang
author_sort Yang, Ming-Hui
collection PubMed
description The eye contains numerous water channel proteins and the roles of AQPs (aquaporins) in the retina are blurred, especially under disease conditions. The purpose of this study was to investigate the expression of AQP9 gene and proteins affected by elevated IOP (intraocular pressure) in a rat model of glaucoma induced by intravitreous injection of hypertonic saline into the episcleral veins. The gene and protein expressions of AQP9 were investigated by real-time PCR and Western blotting. The immunoreactive expression of AQP9, AQP4 and GFAP (glial fibrillary acidic protein) in the optic nerve of rats exposed to experimentally elevated IOP was detected by immunofluorescence microscopy. The mRNA and protein expression levels of AQP9 were up-regulated in the retina of an animal model of glaucoma. The immunoreactivities of the AQP9, AQP4 and GFAP were also detected and increased in the optic nerve region. The expression of AQP9 was up-regulated in this glaucoma model and the immunoreactivities of the AQP4 and GFAP were also detected as co-localizing with AQP9 in the optic nerve region, indicating retina ganglion cells were surrounded by activated astrocytes. This may indicate that the injured neurons may rely on the astrocytes. The alterations of AQP expression may compensate the glaucomatous damage.
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spelling pubmed-36365222013-05-01 Changes in retinal aquaporin-9 (AQP9) expression in glaucoma Yang, Ming-Hui Dibas, Adnan Tyan, Yu-Chang Biosci Rep Original Paper The eye contains numerous water channel proteins and the roles of AQPs (aquaporins) in the retina are blurred, especially under disease conditions. The purpose of this study was to investigate the expression of AQP9 gene and proteins affected by elevated IOP (intraocular pressure) in a rat model of glaucoma induced by intravitreous injection of hypertonic saline into the episcleral veins. The gene and protein expressions of AQP9 were investigated by real-time PCR and Western blotting. The immunoreactive expression of AQP9, AQP4 and GFAP (glial fibrillary acidic protein) in the optic nerve of rats exposed to experimentally elevated IOP was detected by immunofluorescence microscopy. The mRNA and protein expression levels of AQP9 were up-regulated in the retina of an animal model of glaucoma. The immunoreactivities of the AQP9, AQP4 and GFAP were also detected and increased in the optic nerve region. The expression of AQP9 was up-regulated in this glaucoma model and the immunoreactivities of the AQP4 and GFAP were also detected as co-localizing with AQP9 in the optic nerve region, indicating retina ganglion cells were surrounded by activated astrocytes. This may indicate that the injured neurons may rely on the astrocytes. The alterations of AQP expression may compensate the glaucomatous damage. Portland Press Ltd. 2013-04-23 /pmc/articles/PMC3636522/ /pubmed/23464865 http://dx.doi.org/10.1042/BSR20130005 Text en © 2013 The Author(s). http://creativecommons.org/licenses/nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/nc/3.0/) (CC-BY) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Yang, Ming-Hui
Dibas, Adnan
Tyan, Yu-Chang
Changes in retinal aquaporin-9 (AQP9) expression in glaucoma
title Changes in retinal aquaporin-9 (AQP9) expression in glaucoma
title_full Changes in retinal aquaporin-9 (AQP9) expression in glaucoma
title_fullStr Changes in retinal aquaporin-9 (AQP9) expression in glaucoma
title_full_unstemmed Changes in retinal aquaporin-9 (AQP9) expression in glaucoma
title_short Changes in retinal aquaporin-9 (AQP9) expression in glaucoma
title_sort changes in retinal aquaporin-9 (aqp9) expression in glaucoma
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3636522/
https://www.ncbi.nlm.nih.gov/pubmed/23464865
http://dx.doi.org/10.1042/BSR20130005
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