Cargando…
Comparison of activity indexes for recognizing enzyme mutants of higher activity with uricase as model
BACKGROUND: For screening a library of enzyme mutants, an efficient and cost-effective method for reliable assay of enzyme activity and a decision method for safe recognition of mutants of higher activity are needed. The comparison of activity concentrations of mutants in lysates of transformed Esch...
Autores principales: | Feng, Juan, Liu, Hongbo, Yang, Xiaolan, Gao, Ang, Liao, Juan, Feng, Liping, Pu, Jun, Xie, Yanling, Long, Gaobo, Li, Yuanli, Liao, Fei |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637054/ https://www.ncbi.nlm.nih.gov/pubmed/23594729 http://dx.doi.org/10.1186/1752-153X-7-69 |
Ejemplares similares
-
Facile spectrophotometric assay of molar equivalents of
N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol)
derivatives
por: Gao, Ang, et al.
Publicado: (2012) -
Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag
por: Li, Yaping, et al.
Publicado: (2019) -
Impact of Large Aggregated Uricases and PEG Diol on Accelerated Blood Clearance of PEGylated Canine Uricase
por: Zhang, Chun, et al.
Publicado: (2012) -
Data for high-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins
por: Feng, Yiran, et al.
Publicado: (2017) -
Integration of Kinetic Analysis of Reaction Curve with a Proper Classical Approach for Enzymatic Analysis
por: Yang, Xiaolan, et al.
Publicado: (2012)