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Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis

BACKGROUND: Even though more than 600 stroke treatments have been shown effective in preclinical studies, clinically proven treatment alternatives for cerebral infarction remain scarce. Amongst the reasons for the discrepancy may be methodological shortcomings, such as high mortality and outcome var...

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Autores principales: Ström, Jakob O, Ingberg, Edvin, Theodorsson, Annette, Theodorsson, Elvar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637133/
https://www.ncbi.nlm.nih.gov/pubmed/23548160
http://dx.doi.org/10.1186/1471-2202-14-41
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author Ström, Jakob O
Ingberg, Edvin
Theodorsson, Annette
Theodorsson, Elvar
author_facet Ström, Jakob O
Ingberg, Edvin
Theodorsson, Annette
Theodorsson, Elvar
author_sort Ström, Jakob O
collection PubMed
description BACKGROUND: Even though more than 600 stroke treatments have been shown effective in preclinical studies, clinically proven treatment alternatives for cerebral infarction remain scarce. Amongst the reasons for the discrepancy may be methodological shortcomings, such as high mortality and outcome variability, in the preclinical studies. A common approach in animal stroke experiments is that A) focal cerebral ischemia is inflicted, B) some type of treatment is administered and C) the infarct sizes are assessed. However, within this paradigm, the researcher has to make numerous methodological decisions, including choosing rat strain and type of surgical procedure. Even though a few studies have attempted to address the questions experimentally, a lack of consensus regarding the optimal methodology remains. METHODS: We therefore meta-analyzed data from 502 control groups described in 346 articles to find out how rat strain, procedure for causing focal cerebral ischemia and the type of filament coating affected mortality and infarct size variability. RESULTS: The Wistar strain and intraluminal filament procedure using a silicone coated filament was found optimal in lowering infarct size variability. The direct and endothelin methods rendered lower mortality rate, whereas the embolus method increased it compared to the filament method. CONCLUSIONS: The current article provides means for researchers to adjust their middle cerebral artery occlusion (MCAo) protocols to minimize infarct size variability and mortality.
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spelling pubmed-36371332013-04-27 Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis Ström, Jakob O Ingberg, Edvin Theodorsson, Annette Theodorsson, Elvar BMC Neurosci Methodology Article BACKGROUND: Even though more than 600 stroke treatments have been shown effective in preclinical studies, clinically proven treatment alternatives for cerebral infarction remain scarce. Amongst the reasons for the discrepancy may be methodological shortcomings, such as high mortality and outcome variability, in the preclinical studies. A common approach in animal stroke experiments is that A) focal cerebral ischemia is inflicted, B) some type of treatment is administered and C) the infarct sizes are assessed. However, within this paradigm, the researcher has to make numerous methodological decisions, including choosing rat strain and type of surgical procedure. Even though a few studies have attempted to address the questions experimentally, a lack of consensus regarding the optimal methodology remains. METHODS: We therefore meta-analyzed data from 502 control groups described in 346 articles to find out how rat strain, procedure for causing focal cerebral ischemia and the type of filament coating affected mortality and infarct size variability. RESULTS: The Wistar strain and intraluminal filament procedure using a silicone coated filament was found optimal in lowering infarct size variability. The direct and endothelin methods rendered lower mortality rate, whereas the embolus method increased it compared to the filament method. CONCLUSIONS: The current article provides means for researchers to adjust their middle cerebral artery occlusion (MCAo) protocols to minimize infarct size variability and mortality. BioMed Central 2013-04-01 /pmc/articles/PMC3637133/ /pubmed/23548160 http://dx.doi.org/10.1186/1471-2202-14-41 Text en Copyright © 2013 Ström et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Article
Ström, Jakob O
Ingberg, Edvin
Theodorsson, Annette
Theodorsson, Elvar
Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis
title Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis
title_full Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis
title_fullStr Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis
title_full_unstemmed Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis
title_short Method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis
title_sort method parameters’ impact on mortality and variability in rat stroke experiments: a meta-analysis
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637133/
https://www.ncbi.nlm.nih.gov/pubmed/23548160
http://dx.doi.org/10.1186/1471-2202-14-41
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