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Scanning electron microscopy observations of the hedgehog stomach worm, Physaloptera clausa (Spirurida: Physalopteridae)

BACKGROUND: Physaloptera clausa (Spirurida: Physalopteridae) nematodes parasitize the stomach of the European hedgehog (Erinaceus europaeus) and cause weight loss, anorexia and gastric lesions. The present study provides the first morphological description of adult P. clausa from the stomachs of inf...

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Autores principales: Gorgani, Tahmine, Naem, Soraya, Farshid, Amir Abbass, Otranto, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637194/
https://www.ncbi.nlm.nih.gov/pubmed/23566611
http://dx.doi.org/10.1186/1756-3305-6-87
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author Gorgani, Tahmine
Naem, Soraya
Farshid, Amir Abbass
Otranto, Domenico
author_facet Gorgani, Tahmine
Naem, Soraya
Farshid, Amir Abbass
Otranto, Domenico
author_sort Gorgani, Tahmine
collection PubMed
description BACKGROUND: Physaloptera clausa (Spirurida: Physalopteridae) nematodes parasitize the stomach of the European hedgehog (Erinaceus europaeus) and cause weight loss, anorexia and gastric lesions. The present study provides the first morphological description of adult P. clausa from the stomachs of infected hedgehogs, using scanning electron microscopy (SEM). METHODS: From June to October 2011, 10 P. clausa from European hedgehogs were fixed, dried, coated and subjected to SEM examination. RESULTS: Males and females (22–30 mm and 28–47 mm, respectively) were stout, with the cuticle reflecting over the lips to form a large cephalic collarette and showing fine transverse striations in both sexes. The mouth was characterized by two large, simple triangular lateral pseudolabia, each armed with external and internal teeth. Inside the buccal cavity, a circle of internal small teeth can be observed. Around the mouth, four sub-median cephalic papillae and two large amphids were also observed. The anterior end of both male and female bore an excretory pore on the ventral side and a pair of lateral ciliated cervical papillae. In the female worm, the vulva was located in the middle and the eggs were characterized by smooth surfaces. The posterior end of the female worm was stumpy with two large phasmids in proximity to its extremity. The posterior end of the male had large lateral alae, joined together anteriorly across the ventral surface, with subequal and dissimilar spicules, as well as four pairs of stalked pre-cloacal papillae, three pairs of post-cloacal papillae, and two phasmids. Three sessile papillae occured anteriorly and four posteriorly to the cloaca. CONCLUSIONS: The present SEM study provides the first in-depth morphological characterization of adult P. clausa, and highlights similarities and differences with P. bispiculata P. herthameyerae, Heliconema longissimum and Turgida turgida.
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spelling pubmed-36371942013-04-27 Scanning electron microscopy observations of the hedgehog stomach worm, Physaloptera clausa (Spirurida: Physalopteridae) Gorgani, Tahmine Naem, Soraya Farshid, Amir Abbass Otranto, Domenico Parasit Vectors Research BACKGROUND: Physaloptera clausa (Spirurida: Physalopteridae) nematodes parasitize the stomach of the European hedgehog (Erinaceus europaeus) and cause weight loss, anorexia and gastric lesions. The present study provides the first morphological description of adult P. clausa from the stomachs of infected hedgehogs, using scanning electron microscopy (SEM). METHODS: From June to October 2011, 10 P. clausa from European hedgehogs were fixed, dried, coated and subjected to SEM examination. RESULTS: Males and females (22–30 mm and 28–47 mm, respectively) were stout, with the cuticle reflecting over the lips to form a large cephalic collarette and showing fine transverse striations in both sexes. The mouth was characterized by two large, simple triangular lateral pseudolabia, each armed with external and internal teeth. Inside the buccal cavity, a circle of internal small teeth can be observed. Around the mouth, four sub-median cephalic papillae and two large amphids were also observed. The anterior end of both male and female bore an excretory pore on the ventral side and a pair of lateral ciliated cervical papillae. In the female worm, the vulva was located in the middle and the eggs were characterized by smooth surfaces. The posterior end of the female worm was stumpy with two large phasmids in proximity to its extremity. The posterior end of the male had large lateral alae, joined together anteriorly across the ventral surface, with subequal and dissimilar spicules, as well as four pairs of stalked pre-cloacal papillae, three pairs of post-cloacal papillae, and two phasmids. Three sessile papillae occured anteriorly and four posteriorly to the cloaca. CONCLUSIONS: The present SEM study provides the first in-depth morphological characterization of adult P. clausa, and highlights similarities and differences with P. bispiculata P. herthameyerae, Heliconema longissimum and Turgida turgida. BioMed Central 2013-04-08 /pmc/articles/PMC3637194/ /pubmed/23566611 http://dx.doi.org/10.1186/1756-3305-6-87 Text en Copyright © 2013 Gorgani et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Gorgani, Tahmine
Naem, Soraya
Farshid, Amir Abbass
Otranto, Domenico
Scanning electron microscopy observations of the hedgehog stomach worm, Physaloptera clausa (Spirurida: Physalopteridae)
title Scanning electron microscopy observations of the hedgehog stomach worm, Physaloptera clausa (Spirurida: Physalopteridae)
title_full Scanning electron microscopy observations of the hedgehog stomach worm, Physaloptera clausa (Spirurida: Physalopteridae)
title_fullStr Scanning electron microscopy observations of the hedgehog stomach worm, Physaloptera clausa (Spirurida: Physalopteridae)
title_full_unstemmed Scanning electron microscopy observations of the hedgehog stomach worm, Physaloptera clausa (Spirurida: Physalopteridae)
title_short Scanning electron microscopy observations of the hedgehog stomach worm, Physaloptera clausa (Spirurida: Physalopteridae)
title_sort scanning electron microscopy observations of the hedgehog stomach worm, physaloptera clausa (spirurida: physalopteridae)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637194/
https://www.ncbi.nlm.nih.gov/pubmed/23566611
http://dx.doi.org/10.1186/1756-3305-6-87
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