CO(2) Mediated Interaction in Yeast Stimulates Budding and Growth on Minimal Media

Here we show that carbon dioxide (CO(2)) stimulates budding and shortens the lag-period of Saccharomyces cerevisiae cultures, grown on specific weak media. CO(2) can be both exogenous and secreted by another growing yeast culture. We also show that this effect can be observed only in the lag-period, and demonstrate minimal doses and duration of culture exposition to CO(2). Opposite to the effects of CO(2) sensitivity, previously shown for pathogens, where increased concentration of CO(2) suppressed mitosis and stimulated cell differentiation and invasion, here it stimulates budding and culture growth.