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Cholesteryl Esters Accumulate in the Heart in a Porcine Model of Ischemia and Reperfusion
Myocardial ischemia is associated with intracellular accumulation of lipids and increased depots of myocardial lipids are linked to decreased heart function. Despite investigations in cell culture and animal models, there is little data available on where in the heart the lipids accumulate after myo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637450/ https://www.ncbi.nlm.nih.gov/pubmed/23637933 http://dx.doi.org/10.1371/journal.pone.0061942 |
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author | Drevinge, Christina Karlsson, Lars O. Ståhlman, Marcus Larsson, Thomas Perman Sundelin, Jeanna Grip, Lars Andersson, Linda Borén, Jan Levin, Malin C. |
author_facet | Drevinge, Christina Karlsson, Lars O. Ståhlman, Marcus Larsson, Thomas Perman Sundelin, Jeanna Grip, Lars Andersson, Linda Borén, Jan Levin, Malin C. |
author_sort | Drevinge, Christina |
collection | PubMed |
description | Myocardial ischemia is associated with intracellular accumulation of lipids and increased depots of myocardial lipids are linked to decreased heart function. Despite investigations in cell culture and animal models, there is little data available on where in the heart the lipids accumulate after myocardial ischemia and which lipid species that accumulate. The aim of this study was to investigate derangements of lipid metabolism that are associated with myocardial ischemia in a porcine model of ischemia and reperfusion. The large pig heart enables the separation of the infarct area with irreversible injury from the area at risk with reversible injury and the unaffected control area. The surviving myocardium bordering the infarct is exposed to mild ischemia and is stressed, but remains viable. We found that cholesteryl esters accumulated in the infarct area as well as in the bordering myocardium. In addition, we found that expression of the low density lipoprotein receptor (LDLr) and the low density lipoprotein receptor-related protein 1 (LRP1) was up-regulated, suggesting that choleteryl ester uptake is mediated via these receptors. Furthermore, we found increased ceramide accumulation, inflammation and endoplasmatic reticulum (ER) stress in the infarcted area of the pig heart. In addition, we found increased levels of inflammation and ER stress in the myocardium bordering the infarct area. Our results indicate that lipid accumulation in the heart is one of the metabolic derangements remaining after ischemia, even in the myocardium bordering the infarct area. Normalizing lipid levels in the myocardium after ischemia would likely improve myocardial function and should therefore be considered as a target for treatment. |
format | Online Article Text |
id | pubmed-3637450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36374502013-05-01 Cholesteryl Esters Accumulate in the Heart in a Porcine Model of Ischemia and Reperfusion Drevinge, Christina Karlsson, Lars O. Ståhlman, Marcus Larsson, Thomas Perman Sundelin, Jeanna Grip, Lars Andersson, Linda Borén, Jan Levin, Malin C. PLoS One Research Article Myocardial ischemia is associated with intracellular accumulation of lipids and increased depots of myocardial lipids are linked to decreased heart function. Despite investigations in cell culture and animal models, there is little data available on where in the heart the lipids accumulate after myocardial ischemia and which lipid species that accumulate. The aim of this study was to investigate derangements of lipid metabolism that are associated with myocardial ischemia in a porcine model of ischemia and reperfusion. The large pig heart enables the separation of the infarct area with irreversible injury from the area at risk with reversible injury and the unaffected control area. The surviving myocardium bordering the infarct is exposed to mild ischemia and is stressed, but remains viable. We found that cholesteryl esters accumulated in the infarct area as well as in the bordering myocardium. In addition, we found that expression of the low density lipoprotein receptor (LDLr) and the low density lipoprotein receptor-related protein 1 (LRP1) was up-regulated, suggesting that choleteryl ester uptake is mediated via these receptors. Furthermore, we found increased ceramide accumulation, inflammation and endoplasmatic reticulum (ER) stress in the infarcted area of the pig heart. In addition, we found increased levels of inflammation and ER stress in the myocardium bordering the infarct area. Our results indicate that lipid accumulation in the heart is one of the metabolic derangements remaining after ischemia, even in the myocardium bordering the infarct area. Normalizing lipid levels in the myocardium after ischemia would likely improve myocardial function and should therefore be considered as a target for treatment. Public Library of Science 2013-04-26 /pmc/articles/PMC3637450/ /pubmed/23637933 http://dx.doi.org/10.1371/journal.pone.0061942 Text en © 2013 Drevinge et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Drevinge, Christina Karlsson, Lars O. Ståhlman, Marcus Larsson, Thomas Perman Sundelin, Jeanna Grip, Lars Andersson, Linda Borén, Jan Levin, Malin C. Cholesteryl Esters Accumulate in the Heart in a Porcine Model of Ischemia and Reperfusion |
title | Cholesteryl Esters Accumulate in the Heart in a Porcine Model of Ischemia and Reperfusion |
title_full | Cholesteryl Esters Accumulate in the Heart in a Porcine Model of Ischemia and Reperfusion |
title_fullStr | Cholesteryl Esters Accumulate in the Heart in a Porcine Model of Ischemia and Reperfusion |
title_full_unstemmed | Cholesteryl Esters Accumulate in the Heart in a Porcine Model of Ischemia and Reperfusion |
title_short | Cholesteryl Esters Accumulate in the Heart in a Porcine Model of Ischemia and Reperfusion |
title_sort | cholesteryl esters accumulate in the heart in a porcine model of ischemia and reperfusion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637450/ https://www.ncbi.nlm.nih.gov/pubmed/23637933 http://dx.doi.org/10.1371/journal.pone.0061942 |
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