Cargando…
Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult
Disturbance in the gut microbial niche by antibiotics like neomycin produces gastrointestinal (GI) disorders. Here, we evaluated the impact of a mixture of extracts of three herbs (Atractylodis Rhizoma Macrocephalae, Massa Medicata Fermentata, and Dolichoris Semen) with known GI protective activitie...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638609/ https://www.ncbi.nlm.nih.gov/pubmed/23690835 http://dx.doi.org/10.1155/2013/161278 |
_version_ | 1782475852178522112 |
---|---|
author | Bose, Shambhunath Han, Kyung-Wan Lee, Myeong-Jong Kim, Hojun |
author_facet | Bose, Shambhunath Han, Kyung-Wan Lee, Myeong-Jong Kim, Hojun |
author_sort | Bose, Shambhunath |
collection | PubMed |
description | Disturbance in the gut microbial niche by antibiotics like neomycin produces gastrointestinal (GI) disorders. Here, we evaluated the impact of a mixture of extracts of three herbs (Atractylodis Rhizoma Macrocephalae, Massa Medicata Fermentata, and Dolichoris Semen) with known GI protective activities, either laboratory unfermented (herbal formulation-1 (HF-1)) or fermented/re-fermented (herbal formulation-2 (HF-2)) on neomycin-treated rats using a commercial Lactobacillus probiotic as a reference. Treatment with neomycin augmented stool water content, decreased fecal population of Lactobacillus spp., changed the histology of intestine without inducing inflammation, reduced the colonic expression of zonula occludens-1 (ZO-1) and claudin-1, and elevated the serum C-reactive protein (CRP) and interferon-gamma (IFN-γ) levels. Coadministration of either HF-2 or probiotic, but not HF-1, restored the fecal content of Lactobacillus spp., normalized the serum CRP level, and significantly increased the colonic expression of ZO-1 and claudin-1 in neomycin-treated rats. The combined treatment with any of the above agents ameliorated the histological changes of cecum and colon in neomycin-treated rats, and the magnitude of this effect was probiotic > HF-2 > HF-1. Our study revealed the intestinal protective effect of a mixture of three herbs against neomycin insult, which is mediated through multiple mechanisms and is potentiated upon prior fermentation/refermentation of the herbs. |
format | Online Article Text |
id | pubmed-3638609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36386092013-05-20 Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult Bose, Shambhunath Han, Kyung-Wan Lee, Myeong-Jong Kim, Hojun Evid Based Complement Alternat Med Research Article Disturbance in the gut microbial niche by antibiotics like neomycin produces gastrointestinal (GI) disorders. Here, we evaluated the impact of a mixture of extracts of three herbs (Atractylodis Rhizoma Macrocephalae, Massa Medicata Fermentata, and Dolichoris Semen) with known GI protective activities, either laboratory unfermented (herbal formulation-1 (HF-1)) or fermented/re-fermented (herbal formulation-2 (HF-2)) on neomycin-treated rats using a commercial Lactobacillus probiotic as a reference. Treatment with neomycin augmented stool water content, decreased fecal population of Lactobacillus spp., changed the histology of intestine without inducing inflammation, reduced the colonic expression of zonula occludens-1 (ZO-1) and claudin-1, and elevated the serum C-reactive protein (CRP) and interferon-gamma (IFN-γ) levels. Coadministration of either HF-2 or probiotic, but not HF-1, restored the fecal content of Lactobacillus spp., normalized the serum CRP level, and significantly increased the colonic expression of ZO-1 and claudin-1 in neomycin-treated rats. The combined treatment with any of the above agents ameliorated the histological changes of cecum and colon in neomycin-treated rats, and the magnitude of this effect was probiotic > HF-2 > HF-1. Our study revealed the intestinal protective effect of a mixture of three herbs against neomycin insult, which is mediated through multiple mechanisms and is potentiated upon prior fermentation/refermentation of the herbs. Hindawi Publishing Corporation 2013 2013-04-11 /pmc/articles/PMC3638609/ /pubmed/23690835 http://dx.doi.org/10.1155/2013/161278 Text en Copyright © 2013 Shambhunath Bose et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bose, Shambhunath Han, Kyung-Wan Lee, Myeong-Jong Kim, Hojun Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult |
title | Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult |
title_full | Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult |
title_fullStr | Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult |
title_full_unstemmed | Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult |
title_short | Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult |
title_sort | intestinal protective effects of herbal-based formulations in rats against neomycin insult |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638609/ https://www.ncbi.nlm.nih.gov/pubmed/23690835 http://dx.doi.org/10.1155/2013/161278 |
work_keys_str_mv | AT boseshambhunath intestinalprotectiveeffectsofherbalbasedformulationsinratsagainstneomycininsult AT hankyungwan intestinalprotectiveeffectsofherbalbasedformulationsinratsagainstneomycininsult AT leemyeongjong intestinalprotectiveeffectsofherbalbasedformulationsinratsagainstneomycininsult AT kimhojun intestinalprotectiveeffectsofherbalbasedformulationsinratsagainstneomycininsult |