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Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans
We isolated a single chemical compound from A. continentalis and identified it to be kaurenoic acid (KA) and investigated the influence of anticariogenic properties. Inhibitory effects of KA on cariogenic properties such as growth, acid production, biofilm formation, and the adherence of S. mutans w...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638610/ https://www.ncbi.nlm.nih.gov/pubmed/23662113 http://dx.doi.org/10.1155/2013/160592 |
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author | Jeong, Seung-Il Kim, Beom-Su Keum, Ki-Suk Lee, Kwang-Hee Kang, Sun-Young Park, Bok-Im Lee, Young-Rae You, Yong-Ouk |
author_facet | Jeong, Seung-Il Kim, Beom-Su Keum, Ki-Suk Lee, Kwang-Hee Kang, Sun-Young Park, Bok-Im Lee, Young-Rae You, Yong-Ouk |
author_sort | Jeong, Seung-Il |
collection | PubMed |
description | We isolated a single chemical compound from A. continentalis and identified it to be kaurenoic acid (KA) and investigated the influence of anticariogenic properties. Inhibitory effects of KA on cariogenic properties such as growth, acid production, biofilm formation, and the adherence of S. mutans were evaluated. Furthermore, real-time PCR analysis was performed to evaluate the influence of KA on the genetic expression of virulence factors. KA significantly inhibited the growth and acid production of S. mutans at 2–4 μg/mL and 4 μg/mL of KA, respectively. Furthermore, the adherence onto S-HAs was inhibited at 3-4 μg/mL of KA and biofilm formation was significantly inhibited when treated with 3 μg/mL KA and completely inhibited at 4 μg/mL. Also, the inhibitory effect of KA on biofilm formation was confirmed by SEM. In confocal laser scanning microscopy, bacterial viability gradually decreased by KA in a dose dependent manner. Real-time PCR analysis showed that the expressions of gtfB, gtfC, gbpB, spaP, brpA, relA, and vicR were significantly decreased in S. mutans when it was treated with KA. These results suggest that KA from A. continentalis may be a useful agent for inhibiting the cariogenic properties of S. mutans. |
format | Online Article Text |
id | pubmed-3638610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36386102013-05-09 Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans Jeong, Seung-Il Kim, Beom-Su Keum, Ki-Suk Lee, Kwang-Hee Kang, Sun-Young Park, Bok-Im Lee, Young-Rae You, Yong-Ouk Evid Based Complement Alternat Med Research Article We isolated a single chemical compound from A. continentalis and identified it to be kaurenoic acid (KA) and investigated the influence of anticariogenic properties. Inhibitory effects of KA on cariogenic properties such as growth, acid production, biofilm formation, and the adherence of S. mutans were evaluated. Furthermore, real-time PCR analysis was performed to evaluate the influence of KA on the genetic expression of virulence factors. KA significantly inhibited the growth and acid production of S. mutans at 2–4 μg/mL and 4 μg/mL of KA, respectively. Furthermore, the adherence onto S-HAs was inhibited at 3-4 μg/mL of KA and biofilm formation was significantly inhibited when treated with 3 μg/mL KA and completely inhibited at 4 μg/mL. Also, the inhibitory effect of KA on biofilm formation was confirmed by SEM. In confocal laser scanning microscopy, bacterial viability gradually decreased by KA in a dose dependent manner. Real-time PCR analysis showed that the expressions of gtfB, gtfC, gbpB, spaP, brpA, relA, and vicR were significantly decreased in S. mutans when it was treated with KA. These results suggest that KA from A. continentalis may be a useful agent for inhibiting the cariogenic properties of S. mutans. Hindawi Publishing Corporation 2013 2013-04-04 /pmc/articles/PMC3638610/ /pubmed/23662113 http://dx.doi.org/10.1155/2013/160592 Text en Copyright © 2013 Seung-Il Jeong et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Jeong, Seung-Il Kim, Beom-Su Keum, Ki-Suk Lee, Kwang-Hee Kang, Sun-Young Park, Bok-Im Lee, Young-Rae You, Yong-Ouk Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans |
title | Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans
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title_full | Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans
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title_fullStr | Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans
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title_full_unstemmed | Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans
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title_short | Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans
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title_sort | kaurenoic acid from aralia continentalis inhibits biofilm formation of streptococcus mutans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638610/ https://www.ncbi.nlm.nih.gov/pubmed/23662113 http://dx.doi.org/10.1155/2013/160592 |
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