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Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays

This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and...

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Autores principales: Lall, Namrita, Henley-Smith, Cynthia Joan, De Canha, Marco Nuno, Oosthuizen, Carel Basson, Berrington, Danielle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638707/
https://www.ncbi.nlm.nih.gov/pubmed/23653650
http://dx.doi.org/10.1155/2013/420601
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author Lall, Namrita
Henley-Smith, Cynthia Joan
De Canha, Marco Nuno
Oosthuizen, Carel Basson
Berrington, Danielle
author_facet Lall, Namrita
Henley-Smith, Cynthia Joan
De Canha, Marco Nuno
Oosthuizen, Carel Basson
Berrington, Danielle
author_sort Lall, Namrita
collection PubMed
description This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulted in a quick and easily distinguishable colour change that allowed for visual readings. INT and Sodium 3′-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate (XTT) are tetrazolium based reagents which are converted to a formazan dye in the presence of metabolically active mitochondria enzyme. For cell viability analysis, reagents XTT and PrestoBlue were compared. PrestoBlue was able to clearly indicate the minimum inhibitory concentration (MIC) of various positive drug controls on various microbial strains. PrestoBlue was also a good indicator of the 50% inhibitory concentration (IC(50)) of positive drug controls on various cell lines.
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spelling pubmed-36387072013-05-07 Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays Lall, Namrita Henley-Smith, Cynthia Joan De Canha, Marco Nuno Oosthuizen, Carel Basson Berrington, Danielle Int J Microbiol Research Article This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulted in a quick and easily distinguishable colour change that allowed for visual readings. INT and Sodium 3′-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate (XTT) are tetrazolium based reagents which are converted to a formazan dye in the presence of metabolically active mitochondria enzyme. For cell viability analysis, reagents XTT and PrestoBlue were compared. PrestoBlue was able to clearly indicate the minimum inhibitory concentration (MIC) of various positive drug controls on various microbial strains. PrestoBlue was also a good indicator of the 50% inhibitory concentration (IC(50)) of positive drug controls on various cell lines. Hindawi Publishing Corporation 2013 2013-04-04 /pmc/articles/PMC3638707/ /pubmed/23653650 http://dx.doi.org/10.1155/2013/420601 Text en Copyright © 2013 Namrita Lall et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lall, Namrita
Henley-Smith, Cynthia Joan
De Canha, Marco Nuno
Oosthuizen, Carel Basson
Berrington, Danielle
Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays
title Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays
title_full Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays
title_fullStr Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays
title_full_unstemmed Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays
title_short Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays
title_sort viability reagent, prestoblue, in comparison with other available reagents, utilized in cytotoxicity and antimicrobial assays
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638707/
https://www.ncbi.nlm.nih.gov/pubmed/23653650
http://dx.doi.org/10.1155/2013/420601
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