Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms

A derivative spectrophotometric method was proposed for the simultaneous determination of clindamycin and tretinoin in pharmaceutical dosage forms. The measurement was achieved using the first and second derivative signals of clindamycin at ((1)D) 251 nm and ((2)D) 239 nm and tretinoin at ((1)D) 364...

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Autores principales: Barazandeh Tehrani, Maliheh, Namadchian, Melika, Fadaye Vatan, Sedigheh, Souri, Effat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639150/
https://www.ncbi.nlm.nih.gov/pubmed/23575006
http://dx.doi.org/10.1186/2008-2231-21-29
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author Barazandeh Tehrani, Maliheh
Namadchian, Melika
Fadaye Vatan, Sedigheh
Souri, Effat
author_facet Barazandeh Tehrani, Maliheh
Namadchian, Melika
Fadaye Vatan, Sedigheh
Souri, Effat
author_sort Barazandeh Tehrani, Maliheh
collection PubMed
description A derivative spectrophotometric method was proposed for the simultaneous determination of clindamycin and tretinoin in pharmaceutical dosage forms. The measurement was achieved using the first and second derivative signals of clindamycin at ((1)D) 251 nm and ((2)D) 239 nm and tretinoin at ((1)D) 364 nm and ((2)D) 387 nm. The proposed method showed excellent linearity at both first and second derivative order in the range of 60–1200 and 1.25–25 μg/ml for clindamycin phosphate and tretinoin respectively. The within-day and between-day precision and accuracy was in acceptable range (CV<3.81%, error<3.20%). Good agreement between the found and added concentrations indicates successful application of the proposed method for simultaneous determination of clindamycin and tretinoin in synthetic mixtures and pharmaceutical dosage form.
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spelling pubmed-36391502013-04-30 Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms Barazandeh Tehrani, Maliheh Namadchian, Melika Fadaye Vatan, Sedigheh Souri, Effat Daru Research Article A derivative spectrophotometric method was proposed for the simultaneous determination of clindamycin and tretinoin in pharmaceutical dosage forms. The measurement was achieved using the first and second derivative signals of clindamycin at ((1)D) 251 nm and ((2)D) 239 nm and tretinoin at ((1)D) 364 nm and ((2)D) 387 nm. The proposed method showed excellent linearity at both first and second derivative order in the range of 60–1200 and 1.25–25 μg/ml for clindamycin phosphate and tretinoin respectively. The within-day and between-day precision and accuracy was in acceptable range (CV<3.81%, error<3.20%). Good agreement between the found and added concentrations indicates successful application of the proposed method for simultaneous determination of clindamycin and tretinoin in synthetic mixtures and pharmaceutical dosage form. BioMed Central 2013-04-10 /pmc/articles/PMC3639150/ /pubmed/23575006 http://dx.doi.org/10.1186/2008-2231-21-29 Text en Copyright © 2013 Barazandeh Tehrani et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Barazandeh Tehrani, Maliheh
Namadchian, Melika
Fadaye Vatan, Sedigheh
Souri, Effat
Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms
title Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms
title_full Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms
title_fullStr Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms
title_full_unstemmed Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms
title_short Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms
title_sort derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639150/
https://www.ncbi.nlm.nih.gov/pubmed/23575006
http://dx.doi.org/10.1186/2008-2231-21-29
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