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Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
Norovirus (NoV) P domain complexes, the 24 mer P particles and the P dimers, induced effective humoral immunity, but their role in the cellular immune responses remained unclear. We reported here a study on cellular immune responses of the two P domain complexes in comparison with the virus-like par...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639243/ https://www.ncbi.nlm.nih.gov/pubmed/23638188 http://dx.doi.org/10.1371/journal.pone.0063269 |
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author | Fang, Hao Tan, Ming Xia, Ming Wang, Leyi Jiang, Xi |
author_facet | Fang, Hao Tan, Ming Xia, Ming Wang, Leyi Jiang, Xi |
author_sort | Fang, Hao |
collection | PubMed |
description | Norovirus (NoV) P domain complexes, the 24 mer P particles and the P dimers, induced effective humoral immunity, but their role in the cellular immune responses remained unclear. We reported here a study on cellular immune responses of the two P domain complexes in comparison with the virus-like particle (VLP) of a GII.4 NoV (VA387) in mice. The P domain complexes induced significant central memory CD4(+) T cell phenotypes (CD4(+) CD44(+) CD62L(+) CCR7(+)) and activated polyclonal CD4(+) T cells as shown by production of Interleukin (IL)-2, Interferon (IFN)-γ, and Tumor Necrosis Factor (TNF)-α. Most importantly, VA387-specific CD4(+) T cell epitope induced a production of IFN-γ, indicating an antigen-specific CD4(+) T cell response in P domain complex-immunized mice. Furthermore, P domain complexes efficiently induced bone marrow-derived dendritic cell (BMDC) maturation, evidenced by up-regulation of co-stimulatory and MHC class II molecules, as well as production of IL-12 and IL-1β. Finally, P domain complex-induced mature dendritic cells (DCs) elicited proliferation of specific CD4(+) T cells targeting VA387 P domain. Overall, we conclude that the NoV P domain complexes are efficiently presented by DCs to elicit not only humoral but also cellular immune responses against NoVs. Since the P particle is highly effective for both humoral and cellular immune responses and easily produced in Escherichia coli (E. coli), it is a good choice of vaccine against NoVs and a vaccine platform against other diseases. |
format | Online Article Text |
id | pubmed-3639243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36392432013-05-01 Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity Fang, Hao Tan, Ming Xia, Ming Wang, Leyi Jiang, Xi PLoS One Research Article Norovirus (NoV) P domain complexes, the 24 mer P particles and the P dimers, induced effective humoral immunity, but their role in the cellular immune responses remained unclear. We reported here a study on cellular immune responses of the two P domain complexes in comparison with the virus-like particle (VLP) of a GII.4 NoV (VA387) in mice. The P domain complexes induced significant central memory CD4(+) T cell phenotypes (CD4(+) CD44(+) CD62L(+) CCR7(+)) and activated polyclonal CD4(+) T cells as shown by production of Interleukin (IL)-2, Interferon (IFN)-γ, and Tumor Necrosis Factor (TNF)-α. Most importantly, VA387-specific CD4(+) T cell epitope induced a production of IFN-γ, indicating an antigen-specific CD4(+) T cell response in P domain complex-immunized mice. Furthermore, P domain complexes efficiently induced bone marrow-derived dendritic cell (BMDC) maturation, evidenced by up-regulation of co-stimulatory and MHC class II molecules, as well as production of IL-12 and IL-1β. Finally, P domain complex-induced mature dendritic cells (DCs) elicited proliferation of specific CD4(+) T cells targeting VA387 P domain. Overall, we conclude that the NoV P domain complexes are efficiently presented by DCs to elicit not only humoral but also cellular immune responses against NoVs. Since the P particle is highly effective for both humoral and cellular immune responses and easily produced in Escherichia coli (E. coli), it is a good choice of vaccine against NoVs and a vaccine platform against other diseases. Public Library of Science 2013-04-29 /pmc/articles/PMC3639243/ /pubmed/23638188 http://dx.doi.org/10.1371/journal.pone.0063269 Text en © 2013 Fang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fang, Hao Tan, Ming Xia, Ming Wang, Leyi Jiang, Xi Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity |
title | Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity |
title_full | Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity |
title_fullStr | Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity |
title_full_unstemmed | Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity |
title_short | Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity |
title_sort | norovirus p particle efficiently elicits innate, humoral and cellular immunity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639243/ https://www.ncbi.nlm.nih.gov/pubmed/23638188 http://dx.doi.org/10.1371/journal.pone.0063269 |
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