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Arf1 and Membrane Curvature Cooperate to Recruit Arfaptin2 to Liposomes
Arfaptin2 contains a Bin/Amphiphysin/Rvs (BAR) domain and directly interacts with proteins of the Arf/Arl family in their active GTP-bound state. It has been proposed that BAR domains are able to sense membrane curvature and to induce membrane tubulation. We report here that active Arf1 is required...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639266/ https://www.ncbi.nlm.nih.gov/pubmed/23638170 http://dx.doi.org/10.1371/journal.pone.0062963 |
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author | Ambroggio, Ernesto E. Sillibourne, James Antonny, Bruno Manneville, Jean-Baptiste Goud, Bruno |
author_facet | Ambroggio, Ernesto E. Sillibourne, James Antonny, Bruno Manneville, Jean-Baptiste Goud, Bruno |
author_sort | Ambroggio, Ernesto E. |
collection | PubMed |
description | Arfaptin2 contains a Bin/Amphiphysin/Rvs (BAR) domain and directly interacts with proteins of the Arf/Arl family in their active GTP-bound state. It has been proposed that BAR domains are able to sense membrane curvature and to induce membrane tubulation. We report here that active Arf1 is required for the recruitment of Arfaptin2 to artificial liposomes mimicking the Golgi apparatus lipid composition. The Arf1-dependent recruitment of Arfaptin2 increases with membrane curvature, while the recruitment of Arf1 itself is not sensitive to curvature. At high protein concentrations, the binding of Arfaptin2 induces membrane tubulation. Finally, membrane-bound Arfaptin2 is released from the liposome when ArfGAP1 catalyzes the hydrolysis of GTP to GDP in Arf1. These results show that both Arf1 activation and high membrane curvature are required for efficient recruitment of Arfaptin2 to membranes. |
format | Online Article Text |
id | pubmed-3639266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36392662013-05-01 Arf1 and Membrane Curvature Cooperate to Recruit Arfaptin2 to Liposomes Ambroggio, Ernesto E. Sillibourne, James Antonny, Bruno Manneville, Jean-Baptiste Goud, Bruno PLoS One Research Article Arfaptin2 contains a Bin/Amphiphysin/Rvs (BAR) domain and directly interacts with proteins of the Arf/Arl family in their active GTP-bound state. It has been proposed that BAR domains are able to sense membrane curvature and to induce membrane tubulation. We report here that active Arf1 is required for the recruitment of Arfaptin2 to artificial liposomes mimicking the Golgi apparatus lipid composition. The Arf1-dependent recruitment of Arfaptin2 increases with membrane curvature, while the recruitment of Arf1 itself is not sensitive to curvature. At high protein concentrations, the binding of Arfaptin2 induces membrane tubulation. Finally, membrane-bound Arfaptin2 is released from the liposome when ArfGAP1 catalyzes the hydrolysis of GTP to GDP in Arf1. These results show that both Arf1 activation and high membrane curvature are required for efficient recruitment of Arfaptin2 to membranes. Public Library of Science 2013-04-29 /pmc/articles/PMC3639266/ /pubmed/23638170 http://dx.doi.org/10.1371/journal.pone.0062963 Text en © 2013 Ambroggio et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ambroggio, Ernesto E. Sillibourne, James Antonny, Bruno Manneville, Jean-Baptiste Goud, Bruno Arf1 and Membrane Curvature Cooperate to Recruit Arfaptin2 to Liposomes |
title | Arf1 and Membrane Curvature Cooperate to Recruit Arfaptin2 to Liposomes |
title_full | Arf1 and Membrane Curvature Cooperate to Recruit Arfaptin2 to Liposomes |
title_fullStr | Arf1 and Membrane Curvature Cooperate to Recruit Arfaptin2 to Liposomes |
title_full_unstemmed | Arf1 and Membrane Curvature Cooperate to Recruit Arfaptin2 to Liposomes |
title_short | Arf1 and Membrane Curvature Cooperate to Recruit Arfaptin2 to Liposomes |
title_sort | arf1 and membrane curvature cooperate to recruit arfaptin2 to liposomes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639266/ https://www.ncbi.nlm.nih.gov/pubmed/23638170 http://dx.doi.org/10.1371/journal.pone.0062963 |
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