A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs

There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular natur...

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Detalles Bibliográficos
Autores principales: Dolt, Karamjit Singh, Lawrence, Melanie L., Miller-Hodges, Eve, Slight, Joan, Thornburn, Anna, Devenney, Paul S., Hohenstein, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639282/
https://www.ncbi.nlm.nih.gov/pubmed/23637962
http://dx.doi.org/10.1371/journal.pone.0062054
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author Dolt, Karamjit Singh
Lawrence, Melanie L.
Miller-Hodges, Eve
Slight, Joan
Thornburn, Anna
Devenney, Paul S.
Hohenstein, Peter
author_facet Dolt, Karamjit Singh
Lawrence, Melanie L.
Miller-Hodges, Eve
Slight, Joan
Thornburn, Anna
Devenney, Paul S.
Hohenstein, Peter
author_sort Dolt, Karamjit Singh
collection PubMed
description There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models.
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spelling pubmed-36392822013-05-01 A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs Dolt, Karamjit Singh Lawrence, Melanie L. Miller-Hodges, Eve Slight, Joan Thornburn, Anna Devenney, Paul S. Hohenstein, Peter PLoS One Research Article There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models. Public Library of Science 2013-04-29 /pmc/articles/PMC3639282/ /pubmed/23637962 http://dx.doi.org/10.1371/journal.pone.0062054 Text en © 2013 Dolt et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dolt, Karamjit Singh
Lawrence, Melanie L.
Miller-Hodges, Eve
Slight, Joan
Thornburn, Anna
Devenney, Paul S.
Hohenstein, Peter
A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs
title A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs
title_full A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs
title_fullStr A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs
title_full_unstemmed A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs
title_short A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs
title_sort universal vector for high-efficiency multi-fragment recombineering of bacs and knock-in constructs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639282/
https://www.ncbi.nlm.nih.gov/pubmed/23637962
http://dx.doi.org/10.1371/journal.pone.0062054
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