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A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs
There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular natur...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639282/ https://www.ncbi.nlm.nih.gov/pubmed/23637962 http://dx.doi.org/10.1371/journal.pone.0062054 |