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Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar

BACKGROUND: Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The e...

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Autores principales: Wang, Kun, Yang, Haiyan, Wang, Wei, Sun, Run-cang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639831/
https://www.ncbi.nlm.nih.gov/pubmed/23517540
http://dx.doi.org/10.1186/1754-6834-6-42
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author Wang, Kun
Yang, Haiyan
Wang, Wei
Sun, Run-cang
author_facet Wang, Kun
Yang, Haiyan
Wang, Wei
Sun, Run-cang
author_sort Wang, Kun
collection PubMed
description BACKGROUND: Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The effects of incubation duration on delignification efficiency and structural modification of cellulose were comparably studied, as well as the digestibility of cellulose by simultaneous saccharification and fermentation (SSF). RESULTS: Although microbial pretreatments did not significantly introduce lignin degradation, the data from SSF exhibited higher cellulose conversion (21-75% for biopretreated samples for 4–16 weeks) as compared to the untreated poplar (18%). In spite of the essential maintain of crystallinity, the modification of lignin structure during fungal treatment undoubtedly played a key role in improving cellulose bioconversion rates. Finally, the ethanol concentration of 5.16 g/L was detected in the fermentation broth from the cellulosic sample biodegraded for 16 weeks after 24 h SSF, achieving 34.8% cellulose utilization in poplar. CONCLUSION: The potential fungal pretreatment with Trametes velutina D10149 was firstly explored in this study. It is found that the biopretreatment process had a significant effect on the digestibility of substrate probably due to the removal and unit variation of lignin, since the crystallinities of substrates were rarely changed. Additional investigation is still required especially to improve the selectivity for lignin degradation and optimize the digestibility of cellulose.
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spelling pubmed-36398312013-05-06 Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar Wang, Kun Yang, Haiyan Wang, Wei Sun, Run-cang Biotechnol Biofuels Research BACKGROUND: Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The effects of incubation duration on delignification efficiency and structural modification of cellulose were comparably studied, as well as the digestibility of cellulose by simultaneous saccharification and fermentation (SSF). RESULTS: Although microbial pretreatments did not significantly introduce lignin degradation, the data from SSF exhibited higher cellulose conversion (21-75% for biopretreated samples for 4–16 weeks) as compared to the untreated poplar (18%). In spite of the essential maintain of crystallinity, the modification of lignin structure during fungal treatment undoubtedly played a key role in improving cellulose bioconversion rates. Finally, the ethanol concentration of 5.16 g/L was detected in the fermentation broth from the cellulosic sample biodegraded for 16 weeks after 24 h SSF, achieving 34.8% cellulose utilization in poplar. CONCLUSION: The potential fungal pretreatment with Trametes velutina D10149 was firstly explored in this study. It is found that the biopretreatment process had a significant effect on the digestibility of substrate probably due to the removal and unit variation of lignin, since the crystallinities of substrates were rarely changed. Additional investigation is still required especially to improve the selectivity for lignin degradation and optimize the digestibility of cellulose. BioMed Central 2013-03-21 /pmc/articles/PMC3639831/ /pubmed/23517540 http://dx.doi.org/10.1186/1754-6834-6-42 Text en Copyright © 2013 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Wang, Kun
Yang, Haiyan
Wang, Wei
Sun, Run-cang
Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar
title Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar
title_full Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar
title_fullStr Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar
title_full_unstemmed Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar
title_short Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar
title_sort structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639831/
https://www.ncbi.nlm.nih.gov/pubmed/23517540
http://dx.doi.org/10.1186/1754-6834-6-42
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