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Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model

BACKGROUND: The thoracic or thoracoabdominal aortic aneurysm surgery may cause spinal cord ischemia because of aortic cross-clamping and may result in severe postoperative complications caused by spinal cord injury. Ischemia/reperfusion injury may directly or indirectly be responsible for these comp...

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Autores principales: Lafci, Gokhan, Gedik, Hikmet Selcuk, Korkmaz, Kemal, Erdem, Havva, Cicek, Omer Faruk, Nacar, Osman Arikan, Yildirim, Levent, Kaya, Ertugrul, Ankarali, Handan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639838/
https://www.ncbi.nlm.nih.gov/pubmed/23557242
http://dx.doi.org/10.1186/1749-8090-8-64
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author Lafci, Gokhan
Gedik, Hikmet Selcuk
Korkmaz, Kemal
Erdem, Havva
Cicek, Omer Faruk
Nacar, Osman Arikan
Yildirim, Levent
Kaya, Ertugrul
Ankarali, Handan
author_facet Lafci, Gokhan
Gedik, Hikmet Selcuk
Korkmaz, Kemal
Erdem, Havva
Cicek, Omer Faruk
Nacar, Osman Arikan
Yildirim, Levent
Kaya, Ertugrul
Ankarali, Handan
author_sort Lafci, Gokhan
collection PubMed
description BACKGROUND: The thoracic or thoracoabdominal aortic aneurysm surgery may cause spinal cord ischemia because of aortic cross-clamping and may result in severe postoperative complications caused by spinal cord injury. Ischemia/reperfusion injury may directly or indirectly be responsible for these complications. In this study we sought to determine whether combination of iloprost and montelukast can reduce the ischemia/reperfusion injury of spinal cord in a rat model. METHODS: Medulla spinalis tissue concentrations of interleukin-6 (IL-6), myeloperoxidase (MPO) and heat shock protein 70 (HSP-70) were determined in 3 groups of Spraque Dawley rats: control group (operation with cross clamping and intraperitoneal administration of 0.9% saline, n = 7), sham group (operation without cross clamping, n = 7), and study group (operation with cross-clamping and intraperitoneal administration of iloprost (25 ng/kg) and montelukast (1 mg/kg), n = 7). The abdominal aorta was clamped for 45 minutes, with a proximal (just below the left renal artery) and a distal (just above the aortic bifurcation) clip in control and study groups. Hindlimb motor functions were evaluated at 6, 12, 24, and 48 hours using the Motor Deficit Index score. All rats were sacrificed 48 hours after the procedure and spinal cord tissue levels of myeloperoxidase, interleukin-6, and heat shock protein (HSP-70) were evaluated as markers of oxidative stress and inflammation. Histopathological analyses of spinal cord were also performed. RESULTS: The tissue level of HSP-70 was found to be similar among the 3 groups, however, MPO was highest and IL-6 receptor level was lowest in the control group (p = 0.007 and p = 0.005; respectively). In histopathological examination, there was no significant difference among the groups with respect to the neuronal cell degeneration, edema, or inflammation, but vascular congestion was found to be significantly more prominent in the control group than in the sham or in the study group (p = 0.05). Motor deficit index scores at 24 and 48 hours after ischemia were significantly lower in the study group than in the control group. CONCLUSION: This study suggests that combined use of iloprost and montelukast may reduce ischemic damage in transient spinal cord ischemia and may provide better neurological outcome.
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spelling pubmed-36398382013-05-01 Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model Lafci, Gokhan Gedik, Hikmet Selcuk Korkmaz, Kemal Erdem, Havva Cicek, Omer Faruk Nacar, Osman Arikan Yildirim, Levent Kaya, Ertugrul Ankarali, Handan J Cardiothorac Surg Research Article BACKGROUND: The thoracic or thoracoabdominal aortic aneurysm surgery may cause spinal cord ischemia because of aortic cross-clamping and may result in severe postoperative complications caused by spinal cord injury. Ischemia/reperfusion injury may directly or indirectly be responsible for these complications. In this study we sought to determine whether combination of iloprost and montelukast can reduce the ischemia/reperfusion injury of spinal cord in a rat model. METHODS: Medulla spinalis tissue concentrations of interleukin-6 (IL-6), myeloperoxidase (MPO) and heat shock protein 70 (HSP-70) were determined in 3 groups of Spraque Dawley rats: control group (operation with cross clamping and intraperitoneal administration of 0.9% saline, n = 7), sham group (operation without cross clamping, n = 7), and study group (operation with cross-clamping and intraperitoneal administration of iloprost (25 ng/kg) and montelukast (1 mg/kg), n = 7). The abdominal aorta was clamped for 45 minutes, with a proximal (just below the left renal artery) and a distal (just above the aortic bifurcation) clip in control and study groups. Hindlimb motor functions were evaluated at 6, 12, 24, and 48 hours using the Motor Deficit Index score. All rats were sacrificed 48 hours after the procedure and spinal cord tissue levels of myeloperoxidase, interleukin-6, and heat shock protein (HSP-70) were evaluated as markers of oxidative stress and inflammation. Histopathological analyses of spinal cord were also performed. RESULTS: The tissue level of HSP-70 was found to be similar among the 3 groups, however, MPO was highest and IL-6 receptor level was lowest in the control group (p = 0.007 and p = 0.005; respectively). In histopathological examination, there was no significant difference among the groups with respect to the neuronal cell degeneration, edema, or inflammation, but vascular congestion was found to be significantly more prominent in the control group than in the sham or in the study group (p = 0.05). Motor deficit index scores at 24 and 48 hours after ischemia were significantly lower in the study group than in the control group. CONCLUSION: This study suggests that combined use of iloprost and montelukast may reduce ischemic damage in transient spinal cord ischemia and may provide better neurological outcome. BioMed Central 2013-04-04 /pmc/articles/PMC3639838/ /pubmed/23557242 http://dx.doi.org/10.1186/1749-8090-8-64 Text en Copyright © 2013 Lafci et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lafci, Gokhan
Gedik, Hikmet Selcuk
Korkmaz, Kemal
Erdem, Havva
Cicek, Omer Faruk
Nacar, Osman Arikan
Yildirim, Levent
Kaya, Ertugrul
Ankarali, Handan
Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model
title Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model
title_full Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model
title_fullStr Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model
title_full_unstemmed Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model
title_short Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model
title_sort efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639838/
https://www.ncbi.nlm.nih.gov/pubmed/23557242
http://dx.doi.org/10.1186/1749-8090-8-64
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