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Ser/Thr Kinase-Like Protein of Nicotiana benthamiana Is Involved in the Cell-to-Cell Movement of Bamboo mosaic virus

To investigate the plant genes affected by Bamboo mosaic virus (BaMV) infection, we applied a cDNA-amplified fragment length polymorphism technique to screen genes with differential expression. A serine/threonine kinase-like (NbSTKL) gene of Nicotiana benthamiana is upregulated after BaMV infection....

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Autores principales: Cheng, Shun-Fang, Tsai, Meng-Shan, Huang, Chia-Lin, Huang, Ying-Ping, Chen, I-Hsuan, Lin, Na-Sheng, Hsu, Yau-Heiu, Tsai, Ching-Hsiu, Cheng, Chi-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639906/
https://www.ncbi.nlm.nih.gov/pubmed/23646157
http://dx.doi.org/10.1371/journal.pone.0062907
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author Cheng, Shun-Fang
Tsai, Meng-Shan
Huang, Chia-Lin
Huang, Ying-Ping
Chen, I-Hsuan
Lin, Na-Sheng
Hsu, Yau-Heiu
Tsai, Ching-Hsiu
Cheng, Chi-Ping
author_facet Cheng, Shun-Fang
Tsai, Meng-Shan
Huang, Chia-Lin
Huang, Ying-Ping
Chen, I-Hsuan
Lin, Na-Sheng
Hsu, Yau-Heiu
Tsai, Ching-Hsiu
Cheng, Chi-Ping
author_sort Cheng, Shun-Fang
collection PubMed
description To investigate the plant genes affected by Bamboo mosaic virus (BaMV) infection, we applied a cDNA-amplified fragment length polymorphism technique to screen genes with differential expression. A serine/threonine kinase-like (NbSTKL) gene of Nicotiana benthamiana is upregulated after BaMV infection. NbSTKL contains the homologous domain of Ser/Thr kinase. Knocking down the expression of NbSTKL by virus-induced gene silencing reduced the accumulation of BaMV in the inoculated leaves but not in the protoplasts. The spread of GFP-expressing BaMV in the inoculated leaves is also impeded by a reduced expression of NbSTKL. These data imply that NbSTKL facilitates the cell-to-cell movement of BaMV. The subcellular localization of NbSTKL is mainly on the cell membrane, which has been confirmed by mutagenesis and fractionation experiments. Combined with the results showing that active site mutation of NbSTKL does not change its subcellular localization but significantly affects BaMV accumulation, we conclude that NbSTKL may regulate BaMV movement on the cell membrane by its kinase-like activity. Moreover, the transient expression of NbSTKL does not significantly affect the accumulation of Cucumber mosaic virus (CMV) and Potato virus X (PVX); thus, NbSTKL might be a specific protein facilitating BaMV movement.
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spelling pubmed-36399062013-05-03 Ser/Thr Kinase-Like Protein of Nicotiana benthamiana Is Involved in the Cell-to-Cell Movement of Bamboo mosaic virus Cheng, Shun-Fang Tsai, Meng-Shan Huang, Chia-Lin Huang, Ying-Ping Chen, I-Hsuan Lin, Na-Sheng Hsu, Yau-Heiu Tsai, Ching-Hsiu Cheng, Chi-Ping PLoS One Research Article To investigate the plant genes affected by Bamboo mosaic virus (BaMV) infection, we applied a cDNA-amplified fragment length polymorphism technique to screen genes with differential expression. A serine/threonine kinase-like (NbSTKL) gene of Nicotiana benthamiana is upregulated after BaMV infection. NbSTKL contains the homologous domain of Ser/Thr kinase. Knocking down the expression of NbSTKL by virus-induced gene silencing reduced the accumulation of BaMV in the inoculated leaves but not in the protoplasts. The spread of GFP-expressing BaMV in the inoculated leaves is also impeded by a reduced expression of NbSTKL. These data imply that NbSTKL facilitates the cell-to-cell movement of BaMV. The subcellular localization of NbSTKL is mainly on the cell membrane, which has been confirmed by mutagenesis and fractionation experiments. Combined with the results showing that active site mutation of NbSTKL does not change its subcellular localization but significantly affects BaMV accumulation, we conclude that NbSTKL may regulate BaMV movement on the cell membrane by its kinase-like activity. Moreover, the transient expression of NbSTKL does not significantly affect the accumulation of Cucumber mosaic virus (CMV) and Potato virus X (PVX); thus, NbSTKL might be a specific protein facilitating BaMV movement. Public Library of Science 2013-04-30 /pmc/articles/PMC3639906/ /pubmed/23646157 http://dx.doi.org/10.1371/journal.pone.0062907 Text en © 2013 Cheng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cheng, Shun-Fang
Tsai, Meng-Shan
Huang, Chia-Lin
Huang, Ying-Ping
Chen, I-Hsuan
Lin, Na-Sheng
Hsu, Yau-Heiu
Tsai, Ching-Hsiu
Cheng, Chi-Ping
Ser/Thr Kinase-Like Protein of Nicotiana benthamiana Is Involved in the Cell-to-Cell Movement of Bamboo mosaic virus
title Ser/Thr Kinase-Like Protein of Nicotiana benthamiana Is Involved in the Cell-to-Cell Movement of Bamboo mosaic virus
title_full Ser/Thr Kinase-Like Protein of Nicotiana benthamiana Is Involved in the Cell-to-Cell Movement of Bamboo mosaic virus
title_fullStr Ser/Thr Kinase-Like Protein of Nicotiana benthamiana Is Involved in the Cell-to-Cell Movement of Bamboo mosaic virus
title_full_unstemmed Ser/Thr Kinase-Like Protein of Nicotiana benthamiana Is Involved in the Cell-to-Cell Movement of Bamboo mosaic virus
title_short Ser/Thr Kinase-Like Protein of Nicotiana benthamiana Is Involved in the Cell-to-Cell Movement of Bamboo mosaic virus
title_sort ser/thr kinase-like protein of nicotiana benthamiana is involved in the cell-to-cell movement of bamboo mosaic virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639906/
https://www.ncbi.nlm.nih.gov/pubmed/23646157
http://dx.doi.org/10.1371/journal.pone.0062907
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