Cargando…
Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations
BACKGROUND: Pulmonary exacerbations (PEx), frequently associated with airway infection and inflammation, are the leading cause of morbidity in cystic fibrosis (CF). Molecular microbiologic approaches detect complex microbiota from CF airway samples taken during PEx. The relationship between airway m...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639911/ https://www.ncbi.nlm.nih.gov/pubmed/23646159 http://dx.doi.org/10.1371/journal.pone.0062917 |
_version_ | 1782476016753573888 |
---|---|
author | Zemanick, Edith T. Harris, J. Kirk Wagner, Brandie D. Robertson, Charles E. Sagel, Scott D. Stevens, Mark J. Accurso, Frank J. Laguna, Theresa A. |
author_facet | Zemanick, Edith T. Harris, J. Kirk Wagner, Brandie D. Robertson, Charles E. Sagel, Scott D. Stevens, Mark J. Accurso, Frank J. Laguna, Theresa A. |
author_sort | Zemanick, Edith T. |
collection | PubMed |
description | BACKGROUND: Pulmonary exacerbations (PEx), frequently associated with airway infection and inflammation, are the leading cause of morbidity in cystic fibrosis (CF). Molecular microbiologic approaches detect complex microbiota from CF airway samples taken during PEx. The relationship between airway microbiota, inflammation, and lung function during CF PEx is not well understood. OBJECTIVE: To determine the relationships between airway microbiota, inflammation, and lung function in CF subjects treated for PEx. METHODS: Expectorated sputum and blood were collected and lung function testing performed in CF subjects during early (0–3d.) and late treatment (>7d.) for PEx. Sputum was analyzed by culture, pyrosequencing of 16S rRNA amplicons, and quantitative PCR for total and specific bacteria. Sputum IL-8 and neutrophil elastase (NE); and circulating C-reactive protein (CRP) were measured. RESULTS: Thirty-seven sputum samples were collected from 21 CF subjects. At early treatment, lower diversity was associated with high relative abundance (RA) of Pseudomonas (r = −0.67, p<0.001), decreased FEV(1%) predicted (r = 0.49, p = 0.03) and increased CRP (r = −0.58, p = 0.01). In contrast to Pseudomonas, obligate and facultative anaerobic genera were associated with less inflammation and higher FEV(1). With treatment, Pseudomonas RA and P. aeruginosa by qPCR decreased while anaerobic genera showed marked variability in response. Change in RA of Prevotella was associated with more variability in FEV(1) response to treatment than Pseudomonas or Staphylococcus. CONCLUSIONS: Anaerobes identified from sputum by sequencing are associated with less inflammation and higher lung function compared to Pseudomonas at early exacerbation. CF PEx treatment results in variable changes of anaerobic genera suggesting the need for larger studies particularly of patients without traditional CF pathogens. |
format | Online Article Text |
id | pubmed-3639911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36399112013-05-03 Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations Zemanick, Edith T. Harris, J. Kirk Wagner, Brandie D. Robertson, Charles E. Sagel, Scott D. Stevens, Mark J. Accurso, Frank J. Laguna, Theresa A. PLoS One Research Article BACKGROUND: Pulmonary exacerbations (PEx), frequently associated with airway infection and inflammation, are the leading cause of morbidity in cystic fibrosis (CF). Molecular microbiologic approaches detect complex microbiota from CF airway samples taken during PEx. The relationship between airway microbiota, inflammation, and lung function during CF PEx is not well understood. OBJECTIVE: To determine the relationships between airway microbiota, inflammation, and lung function in CF subjects treated for PEx. METHODS: Expectorated sputum and blood were collected and lung function testing performed in CF subjects during early (0–3d.) and late treatment (>7d.) for PEx. Sputum was analyzed by culture, pyrosequencing of 16S rRNA amplicons, and quantitative PCR for total and specific bacteria. Sputum IL-8 and neutrophil elastase (NE); and circulating C-reactive protein (CRP) were measured. RESULTS: Thirty-seven sputum samples were collected from 21 CF subjects. At early treatment, lower diversity was associated with high relative abundance (RA) of Pseudomonas (r = −0.67, p<0.001), decreased FEV(1%) predicted (r = 0.49, p = 0.03) and increased CRP (r = −0.58, p = 0.01). In contrast to Pseudomonas, obligate and facultative anaerobic genera were associated with less inflammation and higher FEV(1). With treatment, Pseudomonas RA and P. aeruginosa by qPCR decreased while anaerobic genera showed marked variability in response. Change in RA of Prevotella was associated with more variability in FEV(1) response to treatment than Pseudomonas or Staphylococcus. CONCLUSIONS: Anaerobes identified from sputum by sequencing are associated with less inflammation and higher lung function compared to Pseudomonas at early exacerbation. CF PEx treatment results in variable changes of anaerobic genera suggesting the need for larger studies particularly of patients without traditional CF pathogens. Public Library of Science 2013-04-30 /pmc/articles/PMC3639911/ /pubmed/23646159 http://dx.doi.org/10.1371/journal.pone.0062917 Text en © 2013 Zemanick et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zemanick, Edith T. Harris, J. Kirk Wagner, Brandie D. Robertson, Charles E. Sagel, Scott D. Stevens, Mark J. Accurso, Frank J. Laguna, Theresa A. Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations |
title | Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations |
title_full | Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations |
title_fullStr | Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations |
title_full_unstemmed | Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations |
title_short | Inflammation and Airway Microbiota during Cystic Fibrosis Pulmonary Exacerbations |
title_sort | inflammation and airway microbiota during cystic fibrosis pulmonary exacerbations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639911/ https://www.ncbi.nlm.nih.gov/pubmed/23646159 http://dx.doi.org/10.1371/journal.pone.0062917 |
work_keys_str_mv | AT zemanickeditht inflammationandairwaymicrobiotaduringcysticfibrosispulmonaryexacerbations AT harrisjkirk inflammationandairwaymicrobiotaduringcysticfibrosispulmonaryexacerbations AT wagnerbrandied inflammationandairwaymicrobiotaduringcysticfibrosispulmonaryexacerbations AT robertsoncharlese inflammationandairwaymicrobiotaduringcysticfibrosispulmonaryexacerbations AT sagelscottd inflammationandairwaymicrobiotaduringcysticfibrosispulmonaryexacerbations AT stevensmarkj inflammationandairwaymicrobiotaduringcysticfibrosispulmonaryexacerbations AT accursofrankj inflammationandairwaymicrobiotaduringcysticfibrosispulmonaryexacerbations AT lagunatheresaa inflammationandairwaymicrobiotaduringcysticfibrosispulmonaryexacerbations |