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Toxicant Induced Changes on Delayed Fluorescence Decay Kinetics of Cyanobacteria and Green Algae: A Rapid and Sensitive Biotest
Algal tests have developed into routine tools for testing toxicity of pollutants in aquatic environments. Meanwhile, in addition to algal growth rates, an increasing number of fluorescence based methods are used for rapid and sensitive toxicity measures. The present study stresses the suitability of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639967/ https://www.ncbi.nlm.nih.gov/pubmed/23646185 http://dx.doi.org/10.1371/journal.pone.0063127 |
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author | Leunert, Franziska Grossart, Hans-Peter Gerhardt, Volkmar Eckert, Werner |
author_facet | Leunert, Franziska Grossart, Hans-Peter Gerhardt, Volkmar Eckert, Werner |
author_sort | Leunert, Franziska |
collection | PubMed |
description | Algal tests have developed into routine tools for testing toxicity of pollutants in aquatic environments. Meanwhile, in addition to algal growth rates, an increasing number of fluorescence based methods are used for rapid and sensitive toxicity measures. The present study stresses the suitability of delayed fluorescence (DF) as a promising parameter for biotests. DF is based on the recombination fluorescence at the reaction centre of photosystem II, which is emitted only by photosynthetically active cells. We analyzed the effects of three chemicals (3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), 3,5 Dichlorophenol (3,5 DCP) and copper) on the shape of the DF decay kinetics for potential use in phytoplankton toxicity tests. The short incubation tests were done with four phytoplankton species, with special emphasis on the cyanobacterium Microcystis aeruginosa. All species exhibited a high sensitivity to DCMU, but cyanobacteria were more affected by copper and less by 3,5 DCP than the tested green algae. Analyses of changes in the DF decay curve in response to the added chemicals indicated the feasibility of the DF decay approach as a rapid and sensitive testing tool. |
format | Online Article Text |
id | pubmed-3639967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36399672013-05-03 Toxicant Induced Changes on Delayed Fluorescence Decay Kinetics of Cyanobacteria and Green Algae: A Rapid and Sensitive Biotest Leunert, Franziska Grossart, Hans-Peter Gerhardt, Volkmar Eckert, Werner PLoS One Research Article Algal tests have developed into routine tools for testing toxicity of pollutants in aquatic environments. Meanwhile, in addition to algal growth rates, an increasing number of fluorescence based methods are used for rapid and sensitive toxicity measures. The present study stresses the suitability of delayed fluorescence (DF) as a promising parameter for biotests. DF is based on the recombination fluorescence at the reaction centre of photosystem II, which is emitted only by photosynthetically active cells. We analyzed the effects of three chemicals (3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), 3,5 Dichlorophenol (3,5 DCP) and copper) on the shape of the DF decay kinetics for potential use in phytoplankton toxicity tests. The short incubation tests were done with four phytoplankton species, with special emphasis on the cyanobacterium Microcystis aeruginosa. All species exhibited a high sensitivity to DCMU, but cyanobacteria were more affected by copper and less by 3,5 DCP than the tested green algae. Analyses of changes in the DF decay curve in response to the added chemicals indicated the feasibility of the DF decay approach as a rapid and sensitive testing tool. Public Library of Science 2013-04-30 /pmc/articles/PMC3639967/ /pubmed/23646185 http://dx.doi.org/10.1371/journal.pone.0063127 Text en © 2013 Leunert et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Leunert, Franziska Grossart, Hans-Peter Gerhardt, Volkmar Eckert, Werner Toxicant Induced Changes on Delayed Fluorescence Decay Kinetics of Cyanobacteria and Green Algae: A Rapid and Sensitive Biotest |
title | Toxicant Induced Changes on Delayed Fluorescence Decay Kinetics of Cyanobacteria and Green Algae: A Rapid and Sensitive Biotest |
title_full | Toxicant Induced Changes on Delayed Fluorescence Decay Kinetics of Cyanobacteria and Green Algae: A Rapid and Sensitive Biotest |
title_fullStr | Toxicant Induced Changes on Delayed Fluorescence Decay Kinetics of Cyanobacteria and Green Algae: A Rapid and Sensitive Biotest |
title_full_unstemmed | Toxicant Induced Changes on Delayed Fluorescence Decay Kinetics of Cyanobacteria and Green Algae: A Rapid and Sensitive Biotest |
title_short | Toxicant Induced Changes on Delayed Fluorescence Decay Kinetics of Cyanobacteria and Green Algae: A Rapid and Sensitive Biotest |
title_sort | toxicant induced changes on delayed fluorescence decay kinetics of cyanobacteria and green algae: a rapid and sensitive biotest |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639967/ https://www.ncbi.nlm.nih.gov/pubmed/23646185 http://dx.doi.org/10.1371/journal.pone.0063127 |
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