Cargando…

Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MS(n)

A validated HPLC-DAD-ESI-MS(n) method for the analysis of non-anthocyanin flavonoids was applied to nine different tissues of twelve lotus genotypes of Nelumbo nucifera and N. lutea, together with an optimized anthocyanin extraction and separation protocol for lotus petals. A total of five anthocyan...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Sha, Xiang, Yue, Deng, Jiao, Liu, Yanling, Li, Shaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640015/
https://www.ncbi.nlm.nih.gov/pubmed/23646125
http://dx.doi.org/10.1371/journal.pone.0062291
_version_ 1782476040321368064
author Chen, Sha
Xiang, Yue
Deng, Jiao
Liu, Yanling
Li, Shaohua
author_facet Chen, Sha
Xiang, Yue
Deng, Jiao
Liu, Yanling
Li, Shaohua
author_sort Chen, Sha
collection PubMed
description A validated HPLC-DAD-ESI-MS(n) method for the analysis of non-anthocyanin flavonoids was applied to nine different tissues of twelve lotus genotypes of Nelumbo nucifera and N. lutea, together with an optimized anthocyanin extraction and separation protocol for lotus petals. A total of five anthocyanins and twenty non-anthocyanin flavonoids was identified and quantified. Flavonoid contents and compositions varied with cultivar and tissue and were used as a basis to divide tissues into three groups characterized by kaempferol and quercetin derivatives. Influences on flower petal coloration were investigated by principal components analyses. High contents of kaempferol glycosides were detected in the petals of N. nucifera while high quercetin glycoside concentrations occurred in N. lutea. Based on these results, biosynthetic pathways leading to specific compounds in lotus tissues are deduced through metabolomic analysis of different genotypes and tissues and correlations among flavonoid compounds.
format Online
Article
Text
id pubmed-3640015
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36400152013-05-03 Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MS(n) Chen, Sha Xiang, Yue Deng, Jiao Liu, Yanling Li, Shaohua PLoS One Research Article A validated HPLC-DAD-ESI-MS(n) method for the analysis of non-anthocyanin flavonoids was applied to nine different tissues of twelve lotus genotypes of Nelumbo nucifera and N. lutea, together with an optimized anthocyanin extraction and separation protocol for lotus petals. A total of five anthocyanins and twenty non-anthocyanin flavonoids was identified and quantified. Flavonoid contents and compositions varied with cultivar and tissue and were used as a basis to divide tissues into three groups characterized by kaempferol and quercetin derivatives. Influences on flower petal coloration were investigated by principal components analyses. High contents of kaempferol glycosides were detected in the petals of N. nucifera while high quercetin glycoside concentrations occurred in N. lutea. Based on these results, biosynthetic pathways leading to specific compounds in lotus tissues are deduced through metabolomic analysis of different genotypes and tissues and correlations among flavonoid compounds. Public Library of Science 2013-04-30 /pmc/articles/PMC3640015/ /pubmed/23646125 http://dx.doi.org/10.1371/journal.pone.0062291 Text en © 2013 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Sha
Xiang, Yue
Deng, Jiao
Liu, Yanling
Li, Shaohua
Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MS(n)
title Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MS(n)
title_full Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MS(n)
title_fullStr Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MS(n)
title_full_unstemmed Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MS(n)
title_short Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MS(n)
title_sort simultaneous analysis of anthocyanin and non-anthocyanin flavonoid in various tissues of different lotus (nelumbo) cultivars by hplc-dad-esi-ms(n)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640015/
https://www.ncbi.nlm.nih.gov/pubmed/23646125
http://dx.doi.org/10.1371/journal.pone.0062291
work_keys_str_mv AT chensha simultaneousanalysisofanthocyaninandnonanthocyaninflavonoidinvarioustissuesofdifferentlotusnelumbocultivarsbyhplcdadesimsn
AT xiangyue simultaneousanalysisofanthocyaninandnonanthocyaninflavonoidinvarioustissuesofdifferentlotusnelumbocultivarsbyhplcdadesimsn
AT dengjiao simultaneousanalysisofanthocyaninandnonanthocyaninflavonoidinvarioustissuesofdifferentlotusnelumbocultivarsbyhplcdadesimsn
AT liuyanling simultaneousanalysisofanthocyaninandnonanthocyaninflavonoidinvarioustissuesofdifferentlotusnelumbocultivarsbyhplcdadesimsn
AT lishaohua simultaneousanalysisofanthocyaninandnonanthocyaninflavonoidinvarioustissuesofdifferentlotusnelumbocultivarsbyhplcdadesimsn