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Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii

Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three gen...

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Autores principales: Cai, Liping, Li, Yanyan, Tao, Guanjun, Guo, Wen, Zhang, Chan, Wang, Xiaoyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640386/
https://www.ncbi.nlm.nih.gov/pubmed/23434833
http://dx.doi.org/10.3390/md11020377
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author Cai, Liping
Li, Yanyan
Tao, Guanjun
Guo, Wen
Zhang, Chan
Wang, Xiaoyuan
author_facet Cai, Liping
Li, Yanyan
Tao, Guanjun
Guo, Wen
Zhang, Chan
Wang, Xiaoyuan
author_sort Cai, Liping
collection PubMed
description Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three genes ESA02293, ESA02951 and ESA01386 encoding for the late acyltransferases of lipid A biosynthesis pathway in C. sakazakii. Based on the sequence alignment, proteins YP_001438378.1 encoded by ESA02293, YP_001439016.1 encoded by ESA02951, and YP_001437482.1 encoded by ESA01386 are homologous to E. coli LpxL, LpxP and LpxM, respectively. Functions of the three acyltransferases were confirmed by overexpressing the genes in E. coli, isolating lipid As and analyzing their structures using an ESI/MS. C. sakazakii LpxL and LpxM transfer a C14:0 secondary acyl chain to the 2′- and 3′-position of lipid A, respectively. C. sakazakii LpxP can transfer either a C16:1 or a C14:0 secondary acyl chains to the 2′-position of lipid A.
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spelling pubmed-36403862013-05-02 Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii Cai, Liping Li, Yanyan Tao, Guanjun Guo, Wen Zhang, Chan Wang, Xiaoyuan Mar Drugs Article Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three genes ESA02293, ESA02951 and ESA01386 encoding for the late acyltransferases of lipid A biosynthesis pathway in C. sakazakii. Based on the sequence alignment, proteins YP_001438378.1 encoded by ESA02293, YP_001439016.1 encoded by ESA02951, and YP_001437482.1 encoded by ESA01386 are homologous to E. coli LpxL, LpxP and LpxM, respectively. Functions of the three acyltransferases were confirmed by overexpressing the genes in E. coli, isolating lipid As and analyzing their structures using an ESI/MS. C. sakazakii LpxL and LpxM transfer a C14:0 secondary acyl chain to the 2′- and 3′-position of lipid A, respectively. C. sakazakii LpxP can transfer either a C16:1 or a C14:0 secondary acyl chains to the 2′-position of lipid A. MDPI 2013-01-31 /pmc/articles/PMC3640386/ /pubmed/23434833 http://dx.doi.org/10.3390/md11020377 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Cai, Liping
Li, Yanyan
Tao, Guanjun
Guo, Wen
Zhang, Chan
Wang, Xiaoyuan
Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
title Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
title_full Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
title_fullStr Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
title_full_unstemmed Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
title_short Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
title_sort identification of three genes encoding for the late acyltransferases of lipid a in cronobacter sakazakii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640386/
https://www.ncbi.nlm.nih.gov/pubmed/23434833
http://dx.doi.org/10.3390/md11020377
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