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Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three gen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640386/ https://www.ncbi.nlm.nih.gov/pubmed/23434833 http://dx.doi.org/10.3390/md11020377 |
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author | Cai, Liping Li, Yanyan Tao, Guanjun Guo, Wen Zhang, Chan Wang, Xiaoyuan |
author_facet | Cai, Liping Li, Yanyan Tao, Guanjun Guo, Wen Zhang, Chan Wang, Xiaoyuan |
author_sort | Cai, Liping |
collection | PubMed |
description | Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three genes ESA02293, ESA02951 and ESA01386 encoding for the late acyltransferases of lipid A biosynthesis pathway in C. sakazakii. Based on the sequence alignment, proteins YP_001438378.1 encoded by ESA02293, YP_001439016.1 encoded by ESA02951, and YP_001437482.1 encoded by ESA01386 are homologous to E. coli LpxL, LpxP and LpxM, respectively. Functions of the three acyltransferases were confirmed by overexpressing the genes in E. coli, isolating lipid As and analyzing their structures using an ESI/MS. C. sakazakii LpxL and LpxM transfer a C14:0 secondary acyl chain to the 2′- and 3′-position of lipid A, respectively. C. sakazakii LpxP can transfer either a C16:1 or a C14:0 secondary acyl chains to the 2′-position of lipid A. |
format | Online Article Text |
id | pubmed-3640386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-36403862013-05-02 Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii Cai, Liping Li, Yanyan Tao, Guanjun Guo, Wen Zhang, Chan Wang, Xiaoyuan Mar Drugs Article Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three genes ESA02293, ESA02951 and ESA01386 encoding for the late acyltransferases of lipid A biosynthesis pathway in C. sakazakii. Based on the sequence alignment, proteins YP_001438378.1 encoded by ESA02293, YP_001439016.1 encoded by ESA02951, and YP_001437482.1 encoded by ESA01386 are homologous to E. coli LpxL, LpxP and LpxM, respectively. Functions of the three acyltransferases were confirmed by overexpressing the genes in E. coli, isolating lipid As and analyzing their structures using an ESI/MS. C. sakazakii LpxL and LpxM transfer a C14:0 secondary acyl chain to the 2′- and 3′-position of lipid A, respectively. C. sakazakii LpxP can transfer either a C16:1 or a C14:0 secondary acyl chains to the 2′-position of lipid A. MDPI 2013-01-31 /pmc/articles/PMC3640386/ /pubmed/23434833 http://dx.doi.org/10.3390/md11020377 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Cai, Liping Li, Yanyan Tao, Guanjun Guo, Wen Zhang, Chan Wang, Xiaoyuan Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii |
title | Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii |
title_full | Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii |
title_fullStr | Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii |
title_full_unstemmed | Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii |
title_short | Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii |
title_sort | identification of three genes encoding for the late acyltransferases of lipid a in cronobacter sakazakii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640386/ https://www.ncbi.nlm.nih.gov/pubmed/23434833 http://dx.doi.org/10.3390/md11020377 |
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