Cargando…

Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers

In order to search for sulfated polysaccharides in different invertebrate connective tissues and to examine their biological activities, we have isolated three types of polysaccharides from the body wall of the three sea cucumbers Holothuria edulis, Apostichopus japonicas and Holothuria nobilis. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Lan, Wu, Mingyi, Xu, Li, Lian, Wu, Xiang, Jingying, Lu, Feng, Gao, Na, Xiao, Chuang, Wang, Shengmin, Zhao, Jinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640388/
https://www.ncbi.nlm.nih.gov/pubmed/23385300
http://dx.doi.org/10.3390/md11020399
_version_ 1782267916116295680
author Luo, Lan
Wu, Mingyi
Xu, Li
Lian, Wu
Xiang, Jingying
Lu, Feng
Gao, Na
Xiao, Chuang
Wang, Shengmin
Zhao, Jinhua
author_facet Luo, Lan
Wu, Mingyi
Xu, Li
Lian, Wu
Xiang, Jingying
Lu, Feng
Gao, Na
Xiao, Chuang
Wang, Shengmin
Zhao, Jinhua
author_sort Luo, Lan
collection PubMed
description In order to search for sulfated polysaccharides in different invertebrate connective tissues and to examine their biological activities, we have isolated three types of polysaccharides from the body wall of the three sea cucumbers Holothuria edulis, Apostichopus japonicas and Holothuria nobilis. The physicochemical properties and anticoagulant activities of these polysaccharides were examined and compared. The chemical composition analysis and nuclear magnetic resonance (NMR) analysis indicate that two types of polysaccharides, sulfated fucan and fucosylated chondroitin sulfate (FuCS), were found in all of the three species and in addition a neutral glycan was observed in H. edulis. The neutral α-glucan was firstly obtained from sea cucumber. The same type of polysaccharides from different species of sea cucumbers have similar physicochemical properties and anticoagulant activities, but those of different types of glycans are significantly different, possibly due to their different monosaccharide compositions, electric charges and average molecular weights. The FuCSs have stronger anticoagulant activities than the sulfated fucans, although the molecular sizes of the FuCSs are lower than those of the sulfated fucans, whereas the neutral glucan has no activity, as expected from the absence of sulfate. Thus, anticoagulant activities of the different type of polysaccharides are likely to relate to monosaccharide composition and sulfate content. Preliminary analysis suggests that the sulfation patterns of the FuCSs may result in the difference in anticoagulant activities. Our data could help elucidate the structure-activity relationship of the sea cucumber polysaccharides.
format Online
Article
Text
id pubmed-3640388
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-36403882013-05-02 Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers Luo, Lan Wu, Mingyi Xu, Li Lian, Wu Xiang, Jingying Lu, Feng Gao, Na Xiao, Chuang Wang, Shengmin Zhao, Jinhua Mar Drugs Article In order to search for sulfated polysaccharides in different invertebrate connective tissues and to examine their biological activities, we have isolated three types of polysaccharides from the body wall of the three sea cucumbers Holothuria edulis, Apostichopus japonicas and Holothuria nobilis. The physicochemical properties and anticoagulant activities of these polysaccharides were examined and compared. The chemical composition analysis and nuclear magnetic resonance (NMR) analysis indicate that two types of polysaccharides, sulfated fucan and fucosylated chondroitin sulfate (FuCS), were found in all of the three species and in addition a neutral glycan was observed in H. edulis. The neutral α-glucan was firstly obtained from sea cucumber. The same type of polysaccharides from different species of sea cucumbers have similar physicochemical properties and anticoagulant activities, but those of different types of glycans are significantly different, possibly due to their different monosaccharide compositions, electric charges and average molecular weights. The FuCSs have stronger anticoagulant activities than the sulfated fucans, although the molecular sizes of the FuCSs are lower than those of the sulfated fucans, whereas the neutral glucan has no activity, as expected from the absence of sulfate. Thus, anticoagulant activities of the different type of polysaccharides are likely to relate to monosaccharide composition and sulfate content. Preliminary analysis suggests that the sulfation patterns of the FuCSs may result in the difference in anticoagulant activities. Our data could help elucidate the structure-activity relationship of the sea cucumber polysaccharides. MDPI 2013-02-05 /pmc/articles/PMC3640388/ /pubmed/23385300 http://dx.doi.org/10.3390/md11020399 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Luo, Lan
Wu, Mingyi
Xu, Li
Lian, Wu
Xiang, Jingying
Lu, Feng
Gao, Na
Xiao, Chuang
Wang, Shengmin
Zhao, Jinhua
Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers
title Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers
title_full Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers
title_fullStr Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers
title_full_unstemmed Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers
title_short Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers
title_sort comparison of physicochemical characteristics and anticoagulant activities of polysaccharides from three sea cucumbers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640388/
https://www.ncbi.nlm.nih.gov/pubmed/23385300
http://dx.doi.org/10.3390/md11020399
work_keys_str_mv AT luolan comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT wumingyi comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT xuli comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT lianwu comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT xiangjingying comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT lufeng comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT gaona comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT xiaochuang comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT wangshengmin comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers
AT zhaojinhua comparisonofphysicochemicalcharacteristicsandanticoagulantactivitiesofpolysaccharidesfromthreeseacucumbers