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Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program
Understanding how the limb blastema is established after the initial wound healing response is an important aspect of regeneration research. Here we performed parallel expression profile time courses of healing lateral wounds versus amputated limbs in axolotl. This comparison between wound healing a...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641036/ https://www.ncbi.nlm.nih.gov/pubmed/23658691 http://dx.doi.org/10.1371/journal.pone.0061352 |
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author | Knapp, Dunja Schulz, Herbert Rascon, Cynthia Alexander Volkmer, Michael Scholz, Juliane Nacu, Eugen Le, Mu Novozhilov, Sergey Tazaki, Akira Protze, Stephanie Jacob, Tina Hubner, Norbert Habermann, Bianca Tanaka, Elly M. |
author_facet | Knapp, Dunja Schulz, Herbert Rascon, Cynthia Alexander Volkmer, Michael Scholz, Juliane Nacu, Eugen Le, Mu Novozhilov, Sergey Tazaki, Akira Protze, Stephanie Jacob, Tina Hubner, Norbert Habermann, Bianca Tanaka, Elly M. |
author_sort | Knapp, Dunja |
collection | PubMed |
description | Understanding how the limb blastema is established after the initial wound healing response is an important aspect of regeneration research. Here we performed parallel expression profile time courses of healing lateral wounds versus amputated limbs in axolotl. This comparison between wound healing and regeneration allowed us to identify amputation-specific genes. By clustering the expression profiles of these samples, we could detect three distinguishable phases of gene expression – early wound healing followed by a transition-phase leading to establishment of the limb development program, which correspond to the three phases of limb regeneration that had been defined by morphological criteria. By focusing on the transition-phase, we identified 93 strictly amputation-associated genes many of which are implicated in oxidative-stress response, chromatin modification, epithelial development or limb development. We further classified the genes based on whether they were or were not significantly expressed in the developing limb bud. The specific localization of 53 selected candidates within the blastema was investigated by in situ hybridization. In summary, we identified a set of genes that are expressed specifically during regeneration and are therefore, likely candidates for the regulation of blastema formation. |
format | Online Article Text |
id | pubmed-3641036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36410362013-05-08 Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program Knapp, Dunja Schulz, Herbert Rascon, Cynthia Alexander Volkmer, Michael Scholz, Juliane Nacu, Eugen Le, Mu Novozhilov, Sergey Tazaki, Akira Protze, Stephanie Jacob, Tina Hubner, Norbert Habermann, Bianca Tanaka, Elly M. PLoS One Research Article Understanding how the limb blastema is established after the initial wound healing response is an important aspect of regeneration research. Here we performed parallel expression profile time courses of healing lateral wounds versus amputated limbs in axolotl. This comparison between wound healing and regeneration allowed us to identify amputation-specific genes. By clustering the expression profiles of these samples, we could detect three distinguishable phases of gene expression – early wound healing followed by a transition-phase leading to establishment of the limb development program, which correspond to the three phases of limb regeneration that had been defined by morphological criteria. By focusing on the transition-phase, we identified 93 strictly amputation-associated genes many of which are implicated in oxidative-stress response, chromatin modification, epithelial development or limb development. We further classified the genes based on whether they were or were not significantly expressed in the developing limb bud. The specific localization of 53 selected candidates within the blastema was investigated by in situ hybridization. In summary, we identified a set of genes that are expressed specifically during regeneration and are therefore, likely candidates for the regulation of blastema formation. Public Library of Science 2013-05-01 /pmc/articles/PMC3641036/ /pubmed/23658691 http://dx.doi.org/10.1371/journal.pone.0061352 Text en © 2013 Knapp et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Knapp, Dunja Schulz, Herbert Rascon, Cynthia Alexander Volkmer, Michael Scholz, Juliane Nacu, Eugen Le, Mu Novozhilov, Sergey Tazaki, Akira Protze, Stephanie Jacob, Tina Hubner, Norbert Habermann, Bianca Tanaka, Elly M. Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program |
title | Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program |
title_full | Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program |
title_fullStr | Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program |
title_full_unstemmed | Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program |
title_short | Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program |
title_sort | comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641036/ https://www.ncbi.nlm.nih.gov/pubmed/23658691 http://dx.doi.org/10.1371/journal.pone.0061352 |
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