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Measuring EGFR Separations on Cells with ∼10 nm Resolution via Fluorophore Localization Imaging with Photobleaching
Detecting receptor dimerisation and other forms of clustering on the cell surface depends on methods capable of determining protein-protein separations with high resolution in the ∼10–50 nm range. However, this distance range poses a significant challenge because it is too large for fluorescence res...
Autores principales: | Needham, Sarah R., Hirsch, Michael, Rolfe, Daniel J., Clarke, David T., Zanetti-Domingues, Laura C., Wareham, Richard, Martin-Fernandez, Marisa L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641073/ https://www.ncbi.nlm.nih.gov/pubmed/23650512 http://dx.doi.org/10.1371/journal.pone.0062331 |
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