Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment

To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation an...

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Autores principales: Celay, Jon, Blanco, Idoia, Lázcoz, Paula, Rotinen, Mirja, Castresana, Javier S., Encío, Ignacio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641123/
https://www.ncbi.nlm.nih.gov/pubmed/23650528
http://dx.doi.org/10.1371/journal.pone.0062771
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author Celay, Jon
Blanco, Idoia
Lázcoz, Paula
Rotinen, Mirja
Castresana, Javier S.
Encío, Ignacio
author_facet Celay, Jon
Blanco, Idoia
Lázcoz, Paula
Rotinen, Mirja
Castresana, Javier S.
Encío, Ignacio
author_sort Celay, Jon
collection PubMed
description To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended on the isomer used.
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spelling pubmed-36411232013-05-06 Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment Celay, Jon Blanco, Idoia Lázcoz, Paula Rotinen, Mirja Castresana, Javier S. Encío, Ignacio PLoS One Research Article To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended on the isomer used. Public Library of Science 2013-05-01 /pmc/articles/PMC3641123/ /pubmed/23650528 http://dx.doi.org/10.1371/journal.pone.0062771 Text en © 2013 Celay et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Celay, Jon
Blanco, Idoia
Lázcoz, Paula
Rotinen, Mirja
Castresana, Javier S.
Encío, Ignacio
Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment
title Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment
title_full Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment
title_fullStr Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment
title_full_unstemmed Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment
title_short Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment
title_sort changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641123/
https://www.ncbi.nlm.nih.gov/pubmed/23650528
http://dx.doi.org/10.1371/journal.pone.0062771
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