Barcoding cells using cell-surface programmable DNA-binding domains

We develop here a novel approach to barcode large numbers of cells through cell-surface expression of programmable zinc-finger DNA-binding domains (sZFs). We show sZFs enable double-stranded DNA to sequence-specifically label living cells, and also develop a sequential tagging approach to in situ im...

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Detalles Bibliográficos
Autores principales: Mali, Prashant, Aach, John, Lee, Jehyuk, Levner, Daniel, Nip, Lisa, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641172/
https://www.ncbi.nlm.nih.gov/pubmed/23503053
http://dx.doi.org/10.1038/nmeth.2407
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author Mali, Prashant
Aach, John
Lee, Jehyuk
Levner, Daniel
Nip, Lisa
Church, George M.
author_facet Mali, Prashant
Aach, John
Lee, Jehyuk
Levner, Daniel
Nip, Lisa
Church, George M.
author_sort Mali, Prashant
collection PubMed
description We develop here a novel approach to barcode large numbers of cells through cell-surface expression of programmable zinc-finger DNA-binding domains (sZFs). We show sZFs enable double-stranded DNA to sequence-specifically label living cells, and also develop a sequential tagging approach to in situ image >3 cell types using just 3 fluorophores. Finally we demonstrate their broad versatility through ability to serve as surrogate reporters and facilitate selective cell capture and targeting.
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spelling pubmed-36411722013-11-01 Barcoding cells using cell-surface programmable DNA-binding domains Mali, Prashant Aach, John Lee, Jehyuk Levner, Daniel Nip, Lisa Church, George M. Nat Methods Article We develop here a novel approach to barcode large numbers of cells through cell-surface expression of programmable zinc-finger DNA-binding domains (sZFs). We show sZFs enable double-stranded DNA to sequence-specifically label living cells, and also develop a sequential tagging approach to in situ image >3 cell types using just 3 fluorophores. Finally we demonstrate their broad versatility through ability to serve as surrogate reporters and facilitate selective cell capture and targeting. 2013-03-17 2013-05 /pmc/articles/PMC3641172/ /pubmed/23503053 http://dx.doi.org/10.1038/nmeth.2407 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Mali, Prashant
Aach, John
Lee, Jehyuk
Levner, Daniel
Nip, Lisa
Church, George M.
Barcoding cells using cell-surface programmable DNA-binding domains
title Barcoding cells using cell-surface programmable DNA-binding domains
title_full Barcoding cells using cell-surface programmable DNA-binding domains
title_fullStr Barcoding cells using cell-surface programmable DNA-binding domains
title_full_unstemmed Barcoding cells using cell-surface programmable DNA-binding domains
title_short Barcoding cells using cell-surface programmable DNA-binding domains
title_sort barcoding cells using cell-surface programmable dna-binding domains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641172/
https://www.ncbi.nlm.nih.gov/pubmed/23503053
http://dx.doi.org/10.1038/nmeth.2407
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