Barcoding cells using cell-surface programmable DNA-binding domains

We develop here a novel approach to barcode large numbers of cells through cell-surface expression of programmable zinc-finger DNA-binding domains (sZFs). We show sZFs enable double-stranded DNA to sequence-specifically label living cells, and also develop a sequential tagging approach to in situ im...

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Detalles Bibliográficos
Autores principales: Mali, Prashant, Aach, John, Lee, Jehyuk, Levner, Daniel, Nip, Lisa, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641172/
https://www.ncbi.nlm.nih.gov/pubmed/23503053
http://dx.doi.org/10.1038/nmeth.2407

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641172/
https://www.ncbi.nlm.nih.gov/pubmed/23503053
http://dx.doi.org/10.1038/nmeth.2407

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