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Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants

Cultivars used for wine and table grape have self-fertile hermaphrodite flowers whereas wild European vines and American and Asian species are dioecious, having either male or female flowers. Consistent with previous studies, the flower sex trait was mapped as a single major locus on chromosome 2 ba...

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Autores principales: Battilana, Juri, Lorenzi, Silvia, Moreira, Flavia M., Moreno-Sanz, Paula, Failla, Osvaldo, Emanuelli, Francesco, Grando, M. Stella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Humana Press Inc 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641292/
https://www.ncbi.nlm.nih.gov/pubmed/23532385
http://dx.doi.org/10.1007/s12033-013-9657-5
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author Battilana, Juri
Lorenzi, Silvia
Moreira, Flavia M.
Moreno-Sanz, Paula
Failla, Osvaldo
Emanuelli, Francesco
Grando, M. Stella
author_facet Battilana, Juri
Lorenzi, Silvia
Moreira, Flavia M.
Moreno-Sanz, Paula
Failla, Osvaldo
Emanuelli, Francesco
Grando, M. Stella
author_sort Battilana, Juri
collection PubMed
description Cultivars used for wine and table grape have self-fertile hermaphrodite flowers whereas wild European vines and American and Asian species are dioecious, having either male or female flowers. Consistent with previous studies, the flower sex trait was mapped as a single major locus on chromosome 2 based on a pure Vitis vinifera population segregating for hermaphrodite and female progeny, and a hybrid population producing all three flower sex types. The sex locus was placed between the same SSR and SNP markers on both genetic maps, although abnormal segregation hampered to fine map the genomic region. From a total of 55 possible haplotypes inferred for three SSR markers around the sex locus, in a population of 132 V. sylvestris accessions and 171 V. vinifera cultivars, one of them accounted for 66 % of the hermaphrodite individuals and may be the result of domestication. Specific size variants of the VVIB23 microsatellite sequence within the 3′-UTR of a putative YABBY1 gene were found to be statistically significantly associated with the sex alleles M, H and f; these markers can provide assistance in defining the status of wild grapevine germplasm. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12033-013-9657-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-36412922013-05-02 Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants Battilana, Juri Lorenzi, Silvia Moreira, Flavia M. Moreno-Sanz, Paula Failla, Osvaldo Emanuelli, Francesco Grando, M. Stella Mol Biotechnol Research Cultivars used for wine and table grape have self-fertile hermaphrodite flowers whereas wild European vines and American and Asian species are dioecious, having either male or female flowers. Consistent with previous studies, the flower sex trait was mapped as a single major locus on chromosome 2 based on a pure Vitis vinifera population segregating for hermaphrodite and female progeny, and a hybrid population producing all three flower sex types. The sex locus was placed between the same SSR and SNP markers on both genetic maps, although abnormal segregation hampered to fine map the genomic region. From a total of 55 possible haplotypes inferred for three SSR markers around the sex locus, in a population of 132 V. sylvestris accessions and 171 V. vinifera cultivars, one of them accounted for 66 % of the hermaphrodite individuals and may be the result of domestication. Specific size variants of the VVIB23 microsatellite sequence within the 3′-UTR of a putative YABBY1 gene were found to be statistically significantly associated with the sex alleles M, H and f; these markers can provide assistance in defining the status of wild grapevine germplasm. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12033-013-9657-5) contains supplementary material, which is available to authorized users. Humana Press Inc 2013-03-27 2013 /pmc/articles/PMC3641292/ /pubmed/23532385 http://dx.doi.org/10.1007/s12033-013-9657-5 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research
Battilana, Juri
Lorenzi, Silvia
Moreira, Flavia M.
Moreno-Sanz, Paula
Failla, Osvaldo
Emanuelli, Francesco
Grando, M. Stella
Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants
title Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants
title_full Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants
title_fullStr Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants
title_full_unstemmed Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants
title_short Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants
title_sort linkage mapping and molecular diversity at the flower sex locus in wild and cultivated grapevine reveal a prominent ssr haplotype in hermaphrodite plants
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641292/
https://www.ncbi.nlm.nih.gov/pubmed/23532385
http://dx.doi.org/10.1007/s12033-013-9657-5
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