Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes

Tespa1 has been recently reported to be a critical molecule in T-cell development, however, the precise molecular mechanisms of Tespa1 remain elusive. Here, we demonstrate that Tespa1 shows amino-acid sequence homology to KRAS-induced actin-interacting protein (KRAP), an inositol 1,4,5-trisphosphate receptor (IP(3)R) binding protein, and that Tespa1 physically associates with IP(3)R in T and B lymphocytes. Two-consecutive phenylalanine residues (Phe185/Phe186) in Tespa1, which are conserved between Tespa1 and KRAP, are indispensable for the association between Tespa1 and IP(3)R. These findings suggest that Tespa1 plays critical roles in the immune system through the regulation of the IP(3)R.