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The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER
Heparin-binding epidermal growth factor (EGF)- like growth factor (HB-EGF) is synthesized in the ER, transported along the exocytic pathway, and expressed on the plasma membrane as a type I transmembrane protein. Upon extracellular stimulation, HB-EGF, either proHB-EGF or the shed form HB-EGF-CTF, u...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642169/ https://www.ncbi.nlm.nih.gov/pubmed/23650612 http://dx.doi.org/10.1016/j.fob.2012.09.002 |
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author | Hieda, Miki Koizumi, Michiko Higashi, Chiduru Tachibana, Taro Taguchi, Tomohiko Higashiyama, Shigeki |
author_facet | Hieda, Miki Koizumi, Michiko Higashi, Chiduru Tachibana, Taro Taguchi, Tomohiko Higashiyama, Shigeki |
author_sort | Hieda, Miki |
collection | PubMed |
description | Heparin-binding epidermal growth factor (EGF)- like growth factor (HB-EGF) is synthesized in the ER, transported along the exocytic pathway, and expressed on the plasma membrane as a type I transmembrane protein. Upon extracellular stimulation, HB-EGF, either proHB-EGF or the shed form HB-EGF-CTF, undergoes endocytosis and is then transported retrogradely to the ER. In this study, we showed the essential contribution of the short cytoplasmic tail of HB-EGF (HB-EGF-cyto) to the bidirectional intracellular trafficking between the ER and plasma membrane and revealed several critical amino acids residues that are responsible for internalization from the plasma membrane and ER targeting. We suggest that these anterograde and retrograde sorting signals within HB-EGF-cyto are strictly regulated by protein modification and conformation. |
format | Online Article Text |
id | pubmed-3642169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-36421692013-05-06 The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER Hieda, Miki Koizumi, Michiko Higashi, Chiduru Tachibana, Taro Taguchi, Tomohiko Higashiyama, Shigeki FEBS Open Bio Article Heparin-binding epidermal growth factor (EGF)- like growth factor (HB-EGF) is synthesized in the ER, transported along the exocytic pathway, and expressed on the plasma membrane as a type I transmembrane protein. Upon extracellular stimulation, HB-EGF, either proHB-EGF or the shed form HB-EGF-CTF, undergoes endocytosis and is then transported retrogradely to the ER. In this study, we showed the essential contribution of the short cytoplasmic tail of HB-EGF (HB-EGF-cyto) to the bidirectional intracellular trafficking between the ER and plasma membrane and revealed several critical amino acids residues that are responsible for internalization from the plasma membrane and ER targeting. We suggest that these anterograde and retrograde sorting signals within HB-EGF-cyto are strictly regulated by protein modification and conformation. Elsevier 2012-09-26 /pmc/articles/PMC3642169/ /pubmed/23650612 http://dx.doi.org/10.1016/j.fob.2012.09.002 Text en © 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical Societies. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non- commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Hieda, Miki Koizumi, Michiko Higashi, Chiduru Tachibana, Taro Taguchi, Tomohiko Higashiyama, Shigeki The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER |
title | The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER |
title_full | The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER |
title_fullStr | The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER |
title_full_unstemmed | The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER |
title_short | The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER |
title_sort | cytoplasmic tail of heparin-binding egf-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and er |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642169/ https://www.ncbi.nlm.nih.gov/pubmed/23650612 http://dx.doi.org/10.1016/j.fob.2012.09.002 |
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