Cargando…

Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology

Maternal consumption of a high fat diet during pregnancy increases the offspring risk for obesity. Using a mouse model, we have previously shown that maternal consumption of a high fat (60%) diet leads to global and gene specific decreases in DNA methylation in the brain of the offspring. The presen...

Descripción completa

Detalles Bibliográficos
Autores principales: Carlin, JesseLea, George, Robert, Reyes, Teresa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642194/
https://www.ncbi.nlm.nih.gov/pubmed/23658839
http://dx.doi.org/10.1371/journal.pone.0063549
_version_ 1782268121152749568
author Carlin, JesseLea
George, Robert
Reyes, Teresa M.
author_facet Carlin, JesseLea
George, Robert
Reyes, Teresa M.
author_sort Carlin, JesseLea
collection PubMed
description Maternal consumption of a high fat diet during pregnancy increases the offspring risk for obesity. Using a mouse model, we have previously shown that maternal consumption of a high fat (60%) diet leads to global and gene specific decreases in DNA methylation in the brain of the offspring. The present experiments were designed to attempt to reverse this DNA hypomethylation through supplementation of the maternal diet with methyl donors, and to determine whether methyl donor supplementation could block or attenuate phenotypes associated with maternal consumption of a HF diet. Metabolic and behavioral (fat preference) outcomes were assessed in male and female adult offspring. Expression of the mu-opioid receptor and dopamine transporter mRNA, as well as global DNA methylation were measured in the brain. Supplementation of the maternal diet with methyl donors attenuated the development of some of the adverse effects seen in offspring from dams fed a high fat diet; including weight gain, increased fat preference (males), changes in CNS gene expression and global hypomethylation in the prefrontal cortex. Notable sex differences were observed. These findings identify the importance of balanced methylation status during pregnancy, particularly in the context of a maternal high fat diet, for optimal offspring outcome.
format Online
Article
Text
id pubmed-3642194
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36421942013-05-08 Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology Carlin, JesseLea George, Robert Reyes, Teresa M. PLoS One Research Article Maternal consumption of a high fat diet during pregnancy increases the offspring risk for obesity. Using a mouse model, we have previously shown that maternal consumption of a high fat (60%) diet leads to global and gene specific decreases in DNA methylation in the brain of the offspring. The present experiments were designed to attempt to reverse this DNA hypomethylation through supplementation of the maternal diet with methyl donors, and to determine whether methyl donor supplementation could block or attenuate phenotypes associated with maternal consumption of a HF diet. Metabolic and behavioral (fat preference) outcomes were assessed in male and female adult offspring. Expression of the mu-opioid receptor and dopamine transporter mRNA, as well as global DNA methylation were measured in the brain. Supplementation of the maternal diet with methyl donors attenuated the development of some of the adverse effects seen in offspring from dams fed a high fat diet; including weight gain, increased fat preference (males), changes in CNS gene expression and global hypomethylation in the prefrontal cortex. Notable sex differences were observed. These findings identify the importance of balanced methylation status during pregnancy, particularly in the context of a maternal high fat diet, for optimal offspring outcome. Public Library of Science 2013-05-02 /pmc/articles/PMC3642194/ /pubmed/23658839 http://dx.doi.org/10.1371/journal.pone.0063549 Text en © 2013 Carlin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Carlin, JesseLea
George, Robert
Reyes, Teresa M.
Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology
title Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology
title_full Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology
title_fullStr Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology
title_full_unstemmed Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology
title_short Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology
title_sort methyl donor supplementation blocks the adverse effects of maternal high fat diet on offspring physiology
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642194/
https://www.ncbi.nlm.nih.gov/pubmed/23658839
http://dx.doi.org/10.1371/journal.pone.0063549
work_keys_str_mv AT carlinjesselea methyldonorsupplementationblockstheadverseeffectsofmaternalhighfatdietonoffspringphysiology
AT georgerobert methyldonorsupplementationblockstheadverseeffectsofmaternalhighfatdietonoffspringphysiology
AT reyesteresam methyldonorsupplementationblockstheadverseeffectsofmaternalhighfatdietonoffspringphysiology