Cargando…
Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology
Maternal consumption of a high fat diet during pregnancy increases the offspring risk for obesity. Using a mouse model, we have previously shown that maternal consumption of a high fat (60%) diet leads to global and gene specific decreases in DNA methylation in the brain of the offspring. The presen...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642194/ https://www.ncbi.nlm.nih.gov/pubmed/23658839 http://dx.doi.org/10.1371/journal.pone.0063549 |
_version_ | 1782268121152749568 |
---|---|
author | Carlin, JesseLea George, Robert Reyes, Teresa M. |
author_facet | Carlin, JesseLea George, Robert Reyes, Teresa M. |
author_sort | Carlin, JesseLea |
collection | PubMed |
description | Maternal consumption of a high fat diet during pregnancy increases the offspring risk for obesity. Using a mouse model, we have previously shown that maternal consumption of a high fat (60%) diet leads to global and gene specific decreases in DNA methylation in the brain of the offspring. The present experiments were designed to attempt to reverse this DNA hypomethylation through supplementation of the maternal diet with methyl donors, and to determine whether methyl donor supplementation could block or attenuate phenotypes associated with maternal consumption of a HF diet. Metabolic and behavioral (fat preference) outcomes were assessed in male and female adult offspring. Expression of the mu-opioid receptor and dopamine transporter mRNA, as well as global DNA methylation were measured in the brain. Supplementation of the maternal diet with methyl donors attenuated the development of some of the adverse effects seen in offspring from dams fed a high fat diet; including weight gain, increased fat preference (males), changes in CNS gene expression and global hypomethylation in the prefrontal cortex. Notable sex differences were observed. These findings identify the importance of balanced methylation status during pregnancy, particularly in the context of a maternal high fat diet, for optimal offspring outcome. |
format | Online Article Text |
id | pubmed-3642194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36421942013-05-08 Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology Carlin, JesseLea George, Robert Reyes, Teresa M. PLoS One Research Article Maternal consumption of a high fat diet during pregnancy increases the offspring risk for obesity. Using a mouse model, we have previously shown that maternal consumption of a high fat (60%) diet leads to global and gene specific decreases in DNA methylation in the brain of the offspring. The present experiments were designed to attempt to reverse this DNA hypomethylation through supplementation of the maternal diet with methyl donors, and to determine whether methyl donor supplementation could block or attenuate phenotypes associated with maternal consumption of a HF diet. Metabolic and behavioral (fat preference) outcomes were assessed in male and female adult offspring. Expression of the mu-opioid receptor and dopamine transporter mRNA, as well as global DNA methylation were measured in the brain. Supplementation of the maternal diet with methyl donors attenuated the development of some of the adverse effects seen in offspring from dams fed a high fat diet; including weight gain, increased fat preference (males), changes in CNS gene expression and global hypomethylation in the prefrontal cortex. Notable sex differences were observed. These findings identify the importance of balanced methylation status during pregnancy, particularly in the context of a maternal high fat diet, for optimal offspring outcome. Public Library of Science 2013-05-02 /pmc/articles/PMC3642194/ /pubmed/23658839 http://dx.doi.org/10.1371/journal.pone.0063549 Text en © 2013 Carlin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Carlin, JesseLea George, Robert Reyes, Teresa M. Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology |
title | Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology |
title_full | Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology |
title_fullStr | Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology |
title_full_unstemmed | Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology |
title_short | Methyl Donor Supplementation Blocks the Adverse Effects of Maternal High Fat Diet on Offspring Physiology |
title_sort | methyl donor supplementation blocks the adverse effects of maternal high fat diet on offspring physiology |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642194/ https://www.ncbi.nlm.nih.gov/pubmed/23658839 http://dx.doi.org/10.1371/journal.pone.0063549 |
work_keys_str_mv | AT carlinjesselea methyldonorsupplementationblockstheadverseeffectsofmaternalhighfatdietonoffspringphysiology AT georgerobert methyldonorsupplementationblockstheadverseeffectsofmaternalhighfatdietonoffspringphysiology AT reyesteresam methyldonorsupplementationblockstheadverseeffectsofmaternalhighfatdietonoffspringphysiology |