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Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM

A major hurdle to evolutionary engineering approaches for multigenic phenotypes is the ability to simultaneously modify multiple genes rapidly and selectively. Here, we describe a method for in vivo-targeted mutagenesis in yeast, targeting glycosylases to embedded arrays for mutagenesis (TaGTEAM). B...

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Detalles Bibliográficos
Autores principales:	Finney-Manchester, Shawn P., Maheshri, Narendra
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2013
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643572/ https://www.ncbi.nlm.nih.gov/pubmed/23470991 http://dx.doi.org/10.1093/nar/gkt150

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