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Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer

In previous work we have documented the nuclear translocation of endothelial NOS (eNOS) and its participation in combinatorial complexes with Estrogen Receptor Beta (ERβ) and Hypoxia Inducible Factors (HIFs) that determine localized chromatin remodeling in response to estrogen (E2) and hypoxia stimu...

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Autores principales: Nanni, Simona, Aiello, Aurora, Re, Agnese, Guffanti, Alessandro, Benvenuti, Valentina, Colussi, Claudia, Castro-Vega, Luis Jaime, Felsani, Armando, Londono-Vallejo, Arturo, Capogrossi, Maurizio C., Bacchetti, Silvia, Gaetano, Carlo, Pontecorvi, Alfredo, Farsetti, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643940/
https://www.ncbi.nlm.nih.gov/pubmed/23658738
http://dx.doi.org/10.1371/journal.pone.0062522
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author Nanni, Simona
Aiello, Aurora
Re, Agnese
Guffanti, Alessandro
Benvenuti, Valentina
Colussi, Claudia
Castro-Vega, Luis Jaime
Felsani, Armando
Londono-Vallejo, Arturo
Capogrossi, Maurizio C.
Bacchetti, Silvia
Gaetano, Carlo
Pontecorvi, Alfredo
Farsetti, Antonella
author_facet Nanni, Simona
Aiello, Aurora
Re, Agnese
Guffanti, Alessandro
Benvenuti, Valentina
Colussi, Claudia
Castro-Vega, Luis Jaime
Felsani, Armando
Londono-Vallejo, Arturo
Capogrossi, Maurizio C.
Bacchetti, Silvia
Gaetano, Carlo
Pontecorvi, Alfredo
Farsetti, Antonella
author_sort Nanni, Simona
collection PubMed
description In previous work we have documented the nuclear translocation of endothelial NOS (eNOS) and its participation in combinatorial complexes with Estrogen Receptor Beta (ERβ) and Hypoxia Inducible Factors (HIFs) that determine localized chromatin remodeling in response to estrogen (E2) and hypoxia stimuli, resulting in transcriptional regulation of genes associated with adverse prognosis in prostate cancer (PCa). To explore the role of nuclear eNOS in the acquisition of aggressive phenotype in PCa, we performed ChIP-Sequencing on chromatin-associated eNOS from cells from a primary tumor with poor outcome and from metastatic LNCaP cells. We found that: 1. the eNOS-bound regions (peaks) are widely distributed across the genome encompassing multiple transcription factors binding sites, including Estrogen Response Elements. 2. E2 increased the number of peaks, indicating hormone-dependent eNOS re-localization. 3. Peak distribution was similar with/without E2 with ≈ 55% of them in extragenic DNA regions and an intriguing involvement of the 5′ domain of several miRs deregulated in PCa. Numerous potentially novel eNOS-targeted genes have been identified suggesting that eNOS participates in the regulation of large gene sets. The parallel finding of downregulation of a cluster of miRs, including miR-34a, in PCa cells associated with poor outcome led us to unveil a molecular link between eNOS and SIRT1, an epigenetic regulator of aging and tumorigenicity, negatively regulated by miR-34a and in turn activating eNOS. E2 potentiates miR-34a downregulation thus enhancing SIRT1 expression, depicting a novel eNOS/SIRT1 interplay fine-tuned by E2-activated ER signaling, and suggesting that eNOS may play an important role in aggressive PCa.
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spelling pubmed-36439402013-05-08 Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer Nanni, Simona Aiello, Aurora Re, Agnese Guffanti, Alessandro Benvenuti, Valentina Colussi, Claudia Castro-Vega, Luis Jaime Felsani, Armando Londono-Vallejo, Arturo Capogrossi, Maurizio C. Bacchetti, Silvia Gaetano, Carlo Pontecorvi, Alfredo Farsetti, Antonella PLoS One Research Article In previous work we have documented the nuclear translocation of endothelial NOS (eNOS) and its participation in combinatorial complexes with Estrogen Receptor Beta (ERβ) and Hypoxia Inducible Factors (HIFs) that determine localized chromatin remodeling in response to estrogen (E2) and hypoxia stimuli, resulting in transcriptional regulation of genes associated with adverse prognosis in prostate cancer (PCa). To explore the role of nuclear eNOS in the acquisition of aggressive phenotype in PCa, we performed ChIP-Sequencing on chromatin-associated eNOS from cells from a primary tumor with poor outcome and from metastatic LNCaP cells. We found that: 1. the eNOS-bound regions (peaks) are widely distributed across the genome encompassing multiple transcription factors binding sites, including Estrogen Response Elements. 2. E2 increased the number of peaks, indicating hormone-dependent eNOS re-localization. 3. Peak distribution was similar with/without E2 with ≈ 55% of them in extragenic DNA regions and an intriguing involvement of the 5′ domain of several miRs deregulated in PCa. Numerous potentially novel eNOS-targeted genes have been identified suggesting that eNOS participates in the regulation of large gene sets. The parallel finding of downregulation of a cluster of miRs, including miR-34a, in PCa cells associated with poor outcome led us to unveil a molecular link between eNOS and SIRT1, an epigenetic regulator of aging and tumorigenicity, negatively regulated by miR-34a and in turn activating eNOS. E2 potentiates miR-34a downregulation thus enhancing SIRT1 expression, depicting a novel eNOS/SIRT1 interplay fine-tuned by E2-activated ER signaling, and suggesting that eNOS may play an important role in aggressive PCa. Public Library of Science 2013-05-03 /pmc/articles/PMC3643940/ /pubmed/23658738 http://dx.doi.org/10.1371/journal.pone.0062522 Text en © 2013 Nanni et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nanni, Simona
Aiello, Aurora
Re, Agnese
Guffanti, Alessandro
Benvenuti, Valentina
Colussi, Claudia
Castro-Vega, Luis Jaime
Felsani, Armando
Londono-Vallejo, Arturo
Capogrossi, Maurizio C.
Bacchetti, Silvia
Gaetano, Carlo
Pontecorvi, Alfredo
Farsetti, Antonella
Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer
title Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer
title_full Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer
title_fullStr Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer
title_full_unstemmed Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer
title_short Estrogen-Dependent Dynamic Profile of eNOS-DNA Associations in Prostate Cancer
title_sort estrogen-dependent dynamic profile of enos-dna associations in prostate cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643940/
https://www.ncbi.nlm.nih.gov/pubmed/23658738
http://dx.doi.org/10.1371/journal.pone.0062522
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