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Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats
BACKGROUND/AIMS: Lack of simple and repeatable tests hampers gastric emptying studies in rats. The aim of this study was to adapt the (14)C-octanoate solid gastric emptying breath test for application in rats, and to validate it against radioscintigraphic method. METHODS: After ingestion of a meal c...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Neurogastroenterology and Motility
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3644653/ https://www.ncbi.nlm.nih.gov/pubmed/23667748 http://dx.doi.org/10.5056/jnm.2013.19.2.171 |
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author | Demedts, Ingrid Vanormelingen, Christophe Van Billoen, Hubert Vanuytsel, Tim Farré, Ricard Masaoka, Tatsuhiro Verbruggen, Alfons Verbeke, Kristien Vanden Berghe, Pieter Tack, Jan |
author_facet | Demedts, Ingrid Vanormelingen, Christophe Van Billoen, Hubert Vanuytsel, Tim Farré, Ricard Masaoka, Tatsuhiro Verbruggen, Alfons Verbeke, Kristien Vanden Berghe, Pieter Tack, Jan |
author_sort | Demedts, Ingrid |
collection | PubMed |
description | BACKGROUND/AIMS: Lack of simple and repeatable tests hampers gastric emptying studies in rats. The aim of this study was to adapt the (14)C-octanoate solid gastric emptying breath test for application in rats, and to validate it against radioscintigraphic method. METHODS: After ingestion of a meal containing 3 mCi (99m)Tc and 2 µCi (14)C-octanoate, 23 male Wistar rats were placed on a gamma camera in a airflow container. Scintigraphic images were taken at regular intervals. The amount of (14)CO(2) in a regularly replaced hyamine hydroxide solution, capturing CO(2) in the outflow air, was counted using liquid scintillation spectrometry. (99m)Tc gastric retention curves and (14)CO(2)-excretion curves were fitted to their respective data. Three rats underwent the same procedures after administration of atropine. RESULTS: Overall T(r10%) (time at which 10% of the original amount of (99m)Tc remained in the stomach) was 355 ± 64 minutes; T(e90%) (time at which 90% of total amount of (14)CO(2) was excreted) was 325 ± 106 minutes. Their correlation coefficient was 0.71, R-square 0.50 and P < 0.005. T(r1/2) (50% of original amount of (99m)Tc remained) was 124 ± 28 minutes; T(e1/2) (50% of total amount of (14)CO(2) excreted) 114 ± 32 minutes. Their correlation coefficient was 0.83 with R-square of 0.69 and P < 0.00005. In 12 immobilized animals correlation was even better: correlation coefficient 0.84; R-square 0.71 and P < 0.001 (T(r10%) was 388 ± 117 minutes; T(e90%) 532 ± 219 minutes; T(r1/2) of 165 ± 54 minutes; T(e1/2) of 175 ± 67 minutes). Atropine significantly lengthened all emptying times: 904 ± 307 and 1461 ± 684 minutes for T(r10%) and T(e90%), respectively; and 432 ± 117 minutes for T(r1/2) and 473 ± 190 minutes for T(e1/2). CONCLUSIONS: We adapted and validated the (14)C-octanoate gastric emptying breath test for application in rats. |
format | Online Article Text |
id | pubmed-3644653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Korean Society of Neurogastroenterology and Motility |
record_format | MEDLINE/PubMed |
spelling | pubmed-36446532013-05-10 Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats Demedts, Ingrid Vanormelingen, Christophe Van Billoen, Hubert Vanuytsel, Tim Farré, Ricard Masaoka, Tatsuhiro Verbruggen, Alfons Verbeke, Kristien Vanden Berghe, Pieter Tack, Jan J Neurogastroenterol Motil Original Article BACKGROUND/AIMS: Lack of simple and repeatable tests hampers gastric emptying studies in rats. The aim of this study was to adapt the (14)C-octanoate solid gastric emptying breath test for application in rats, and to validate it against radioscintigraphic method. METHODS: After ingestion of a meal containing 3 mCi (99m)Tc and 2 µCi (14)C-octanoate, 23 male Wistar rats were placed on a gamma camera in a airflow container. Scintigraphic images were taken at regular intervals. The amount of (14)CO(2) in a regularly replaced hyamine hydroxide solution, capturing CO(2) in the outflow air, was counted using liquid scintillation spectrometry. (99m)Tc gastric retention curves and (14)CO(2)-excretion curves were fitted to their respective data. Three rats underwent the same procedures after administration of atropine. RESULTS: Overall T(r10%) (time at which 10% of the original amount of (99m)Tc remained in the stomach) was 355 ± 64 minutes; T(e90%) (time at which 90% of total amount of (14)CO(2) was excreted) was 325 ± 106 minutes. Their correlation coefficient was 0.71, R-square 0.50 and P < 0.005. T(r1/2) (50% of original amount of (99m)Tc remained) was 124 ± 28 minutes; T(e1/2) (50% of total amount of (14)CO(2) excreted) 114 ± 32 minutes. Their correlation coefficient was 0.83 with R-square of 0.69 and P < 0.00005. In 12 immobilized animals correlation was even better: correlation coefficient 0.84; R-square 0.71 and P < 0.001 (T(r10%) was 388 ± 117 minutes; T(e90%) 532 ± 219 minutes; T(r1/2) of 165 ± 54 minutes; T(e1/2) of 175 ± 67 minutes). Atropine significantly lengthened all emptying times: 904 ± 307 and 1461 ± 684 minutes for T(r10%) and T(e90%), respectively; and 432 ± 117 minutes for T(r1/2) and 473 ± 190 minutes for T(e1/2). CONCLUSIONS: We adapted and validated the (14)C-octanoate gastric emptying breath test for application in rats. Korean Society of Neurogastroenterology and Motility 2013-04 2013-04-16 /pmc/articles/PMC3644653/ /pubmed/23667748 http://dx.doi.org/10.5056/jnm.2013.19.2.171 Text en © 2013 The Korean Society of Neurogastroenterology and Motility http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Demedts, Ingrid Vanormelingen, Christophe Van Billoen, Hubert Vanuytsel, Tim Farré, Ricard Masaoka, Tatsuhiro Verbruggen, Alfons Verbeke, Kristien Vanden Berghe, Pieter Tack, Jan Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats |
title | Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats |
title_full | Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats |
title_fullStr | Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats |
title_full_unstemmed | Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats |
title_short | Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats |
title_sort | validation of octanoate breath test for measuring gastric emptying in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3644653/ https://www.ncbi.nlm.nih.gov/pubmed/23667748 http://dx.doi.org/10.5056/jnm.2013.19.2.171 |
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