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Candida albicans susceptibility to lactoperoxidase-generated hypoiodite

In vivo, lactoperoxidase produces hypothiocyanite (OSCN(−)) from thiocyanate (SCN(−)) in the presence of hydrogen peroxide (H(2)O(2)); in vitro, iodide (I(−)) can be oxidized into hypoiodite (OI(−)) by this enzyme. The aim of this study was to compare in vitro the anti-Candida effect of iodide versu...

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Autores principales: Ahariz, Mohamed, Courtois, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645462/
https://www.ncbi.nlm.nih.gov/pubmed/23662084
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author Ahariz, Mohamed
Courtois, Philippe
author_facet Ahariz, Mohamed
Courtois, Philippe
author_sort Ahariz, Mohamed
collection PubMed
description In vivo, lactoperoxidase produces hypothiocyanite (OSCN(−)) from thiocyanate (SCN(−)) in the presence of hydrogen peroxide (H(2)O(2)); in vitro, iodide (I(−)) can be oxidized into hypoiodite (OI(−)) by this enzyme. The aim of this study was to compare in vitro the anti-Candida effect of iodide versus thiocyanate used as lactoperoxidase substrate to prevent Candida biofilms development. Candida albicans ATCC 10231 susceptibility upon both peroxidase systems was tested in three different experimental designs: (i) in a liquid culture medium, (ii) in an interface model between solid culture medium and gel containing the enzymic systems, (iii) in a biofilm model onto titanium and acrylic resin. Yeast growth in liquid medium was monitored by turbidimetry at 600 nm. Material-adherent yeast biomass was evaluated by the tetrazolium salt MTT method. The iodide-peroxidase system has been shown to inhibit Candida biofilm formation at lower substrate concentrations (~200 fold less H(2)O(2) donor) and for longer incubation periods than the thiocyanate-peroxidase system. In conclusion, efficiency of lactoperoxidase-generated OI(−) to prevent C. albicans biofilm development allows refining iodine antifungal use in ex vivo conditions.
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spelling pubmed-36454622013-05-09 Candida albicans susceptibility to lactoperoxidase-generated hypoiodite Ahariz, Mohamed Courtois, Philippe Clin Cosmet Investig Dent Original Research In vivo, lactoperoxidase produces hypothiocyanite (OSCN(−)) from thiocyanate (SCN(−)) in the presence of hydrogen peroxide (H(2)O(2)); in vitro, iodide (I(−)) can be oxidized into hypoiodite (OI(−)) by this enzyme. The aim of this study was to compare in vitro the anti-Candida effect of iodide versus thiocyanate used as lactoperoxidase substrate to prevent Candida biofilms development. Candida albicans ATCC 10231 susceptibility upon both peroxidase systems was tested in three different experimental designs: (i) in a liquid culture medium, (ii) in an interface model between solid culture medium and gel containing the enzymic systems, (iii) in a biofilm model onto titanium and acrylic resin. Yeast growth in liquid medium was monitored by turbidimetry at 600 nm. Material-adherent yeast biomass was evaluated by the tetrazolium salt MTT method. The iodide-peroxidase system has been shown to inhibit Candida biofilm formation at lower substrate concentrations (~200 fold less H(2)O(2) donor) and for longer incubation periods than the thiocyanate-peroxidase system. In conclusion, efficiency of lactoperoxidase-generated OI(−) to prevent C. albicans biofilm development allows refining iodine antifungal use in ex vivo conditions. Dove Medical Press 2010-08-04 /pmc/articles/PMC3645462/ /pubmed/23662084 Text en © 2010 Ahariz and Courtois, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Ahariz, Mohamed
Courtois, Philippe
Candida albicans susceptibility to lactoperoxidase-generated hypoiodite
title Candida albicans susceptibility to lactoperoxidase-generated hypoiodite
title_full Candida albicans susceptibility to lactoperoxidase-generated hypoiodite
title_fullStr Candida albicans susceptibility to lactoperoxidase-generated hypoiodite
title_full_unstemmed Candida albicans susceptibility to lactoperoxidase-generated hypoiodite
title_short Candida albicans susceptibility to lactoperoxidase-generated hypoiodite
title_sort candida albicans susceptibility to lactoperoxidase-generated hypoiodite
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645462/
https://www.ncbi.nlm.nih.gov/pubmed/23662084
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