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Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines

Receptor for Advanced Glycation End Products (RAGE) is an oncogenic trans-membranous receptor overexpressed in various human cancers. However, the role of RAGE in breast cancer development and proliferation is still unclear. In this study, we demonstrated that RAGE expression levels are correlated t...

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Autores principales: Radia, AL-Madhagi, Yaser, AL-Madhagi, Ma, Xiaoqian, Zhang, Juan, Yang, Cejun, Dong, Qiong, Rong, Pengfei, Ye, Bin, Liu, Sheng, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645726/
https://www.ncbi.nlm.nih.gov/pubmed/23579957
http://dx.doi.org/10.3390/ijms14047959
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author Radia, AL-Madhagi
Yaser, AL-Madhagi
Ma, Xiaoqian
Zhang, Juan
Yang, Cejun
Dong, Qiong
Rong, Pengfei
Ye, Bin
Liu, Sheng
Wang, Wei
author_facet Radia, AL-Madhagi
Yaser, AL-Madhagi
Ma, Xiaoqian
Zhang, Juan
Yang, Cejun
Dong, Qiong
Rong, Pengfei
Ye, Bin
Liu, Sheng
Wang, Wei
author_sort Radia, AL-Madhagi
collection PubMed
description Receptor for Advanced Glycation End Products (RAGE) is an oncogenic trans-membranous receptor overexpressed in various human cancers. However, the role of RAGE in breast cancer development and proliferation is still unclear. In this study, we demonstrated that RAGE expression levels are correlated to the degree of severity of breast cancer. Furthermore, there is a decrease in the proliferation of all sub-types of breast cancer, MCF-7, SK-Br-3 and MDA-MB-231, as a result of the effect of RAGE siRNA. RAGE siRNA arrested cells in the G1 phase and inhibited DNA synthesis (p < 0.05). Moreover, qRT-PCR and Western Blot results demonstrated that RAGE siRNA decreases the expression of transcriptional factor NF-κB p65 as well as the expression of cell proliferation markers PCNA and cyclinD1. RAGE and RAGE ligands can thus be considered as possible targets for breast cancer management and therapy.
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spelling pubmed-36457262013-05-13 Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines Radia, AL-Madhagi Yaser, AL-Madhagi Ma, Xiaoqian Zhang, Juan Yang, Cejun Dong, Qiong Rong, Pengfei Ye, Bin Liu, Sheng Wang, Wei Int J Mol Sci Article Receptor for Advanced Glycation End Products (RAGE) is an oncogenic trans-membranous receptor overexpressed in various human cancers. However, the role of RAGE in breast cancer development and proliferation is still unclear. In this study, we demonstrated that RAGE expression levels are correlated to the degree of severity of breast cancer. Furthermore, there is a decrease in the proliferation of all sub-types of breast cancer, MCF-7, SK-Br-3 and MDA-MB-231, as a result of the effect of RAGE siRNA. RAGE siRNA arrested cells in the G1 phase and inhibited DNA synthesis (p < 0.05). Moreover, qRT-PCR and Western Blot results demonstrated that RAGE siRNA decreases the expression of transcriptional factor NF-κB p65 as well as the expression of cell proliferation markers PCNA and cyclinD1. RAGE and RAGE ligands can thus be considered as possible targets for breast cancer management and therapy. Molecular Diversity Preservation International (MDPI) 2013-04-11 /pmc/articles/PMC3645726/ /pubmed/23579957 http://dx.doi.org/10.3390/ijms14047959 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Radia, AL-Madhagi
Yaser, AL-Madhagi
Ma, Xiaoqian
Zhang, Juan
Yang, Cejun
Dong, Qiong
Rong, Pengfei
Ye, Bin
Liu, Sheng
Wang, Wei
Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines
title Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines
title_full Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines
title_fullStr Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines
title_full_unstemmed Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines
title_short Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines
title_sort specific sirna targeting receptor for advanced glycation end products (rage) decreases proliferation in human breast cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645726/
https://www.ncbi.nlm.nih.gov/pubmed/23579957
http://dx.doi.org/10.3390/ijms14047959
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