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Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines
Receptor for Advanced Glycation End Products (RAGE) is an oncogenic trans-membranous receptor overexpressed in various human cancers. However, the role of RAGE in breast cancer development and proliferation is still unclear. In this study, we demonstrated that RAGE expression levels are correlated t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645726/ https://www.ncbi.nlm.nih.gov/pubmed/23579957 http://dx.doi.org/10.3390/ijms14047959 |
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author | Radia, AL-Madhagi Yaser, AL-Madhagi Ma, Xiaoqian Zhang, Juan Yang, Cejun Dong, Qiong Rong, Pengfei Ye, Bin Liu, Sheng Wang, Wei |
author_facet | Radia, AL-Madhagi Yaser, AL-Madhagi Ma, Xiaoqian Zhang, Juan Yang, Cejun Dong, Qiong Rong, Pengfei Ye, Bin Liu, Sheng Wang, Wei |
author_sort | Radia, AL-Madhagi |
collection | PubMed |
description | Receptor for Advanced Glycation End Products (RAGE) is an oncogenic trans-membranous receptor overexpressed in various human cancers. However, the role of RAGE in breast cancer development and proliferation is still unclear. In this study, we demonstrated that RAGE expression levels are correlated to the degree of severity of breast cancer. Furthermore, there is a decrease in the proliferation of all sub-types of breast cancer, MCF-7, SK-Br-3 and MDA-MB-231, as a result of the effect of RAGE siRNA. RAGE siRNA arrested cells in the G1 phase and inhibited DNA synthesis (p < 0.05). Moreover, qRT-PCR and Western Blot results demonstrated that RAGE siRNA decreases the expression of transcriptional factor NF-κB p65 as well as the expression of cell proliferation markers PCNA and cyclinD1. RAGE and RAGE ligands can thus be considered as possible targets for breast cancer management and therapy. |
format | Online Article Text |
id | pubmed-3645726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36457262013-05-13 Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines Radia, AL-Madhagi Yaser, AL-Madhagi Ma, Xiaoqian Zhang, Juan Yang, Cejun Dong, Qiong Rong, Pengfei Ye, Bin Liu, Sheng Wang, Wei Int J Mol Sci Article Receptor for Advanced Glycation End Products (RAGE) is an oncogenic trans-membranous receptor overexpressed in various human cancers. However, the role of RAGE in breast cancer development and proliferation is still unclear. In this study, we demonstrated that RAGE expression levels are correlated to the degree of severity of breast cancer. Furthermore, there is a decrease in the proliferation of all sub-types of breast cancer, MCF-7, SK-Br-3 and MDA-MB-231, as a result of the effect of RAGE siRNA. RAGE siRNA arrested cells in the G1 phase and inhibited DNA synthesis (p < 0.05). Moreover, qRT-PCR and Western Blot results demonstrated that RAGE siRNA decreases the expression of transcriptional factor NF-κB p65 as well as the expression of cell proliferation markers PCNA and cyclinD1. RAGE and RAGE ligands can thus be considered as possible targets for breast cancer management and therapy. Molecular Diversity Preservation International (MDPI) 2013-04-11 /pmc/articles/PMC3645726/ /pubmed/23579957 http://dx.doi.org/10.3390/ijms14047959 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Radia, AL-Madhagi Yaser, AL-Madhagi Ma, Xiaoqian Zhang, Juan Yang, Cejun Dong, Qiong Rong, Pengfei Ye, Bin Liu, Sheng Wang, Wei Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines |
title | Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines |
title_full | Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines |
title_fullStr | Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines |
title_full_unstemmed | Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines |
title_short | Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines |
title_sort | specific sirna targeting receptor for advanced glycation end products (rage) decreases proliferation in human breast cancer cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645726/ https://www.ncbi.nlm.nih.gov/pubmed/23579957 http://dx.doi.org/10.3390/ijms14047959 |
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