Cargando…
Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor
Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent an important environmental constraint limiting crop productivity. In this study changes in proteome of leaves and crowns in a frost tolerant winter barley cv. Luxor in rela...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645728/ https://www.ncbi.nlm.nih.gov/pubmed/23584021 http://dx.doi.org/10.3390/ijms14048000 |
_version_ | 1782268532584611840 |
---|---|
author | Hlaváčková, Iva Vítámvás, Pavel Šantrůček, Jiří Kosová, Klára Zelenková, Sylva Prášil, Ilja Tom Ovesná, Jaroslava Hynek, Radovan Kodíček, Milan |
author_facet | Hlaváčková, Iva Vítámvás, Pavel Šantrůček, Jiří Kosová, Klára Zelenková, Sylva Prášil, Ilja Tom Ovesná, Jaroslava Hynek, Radovan Kodíček, Milan |
author_sort | Hlaváčková, Iva |
collection | PubMed |
description | Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent an important environmental constraint limiting crop productivity. In this study changes in proteome of leaves and crowns in a frost tolerant winter barley cv. Luxor in relation to short and long term periods of cold followed by a brief frost treatment were studied in order to disclose proteins responsible for the cold hardening process in distinct plant tissues. The mentioned changes have been monitored using two dimensional difference gel electrophoresis (2D-DIGE) with subsequent peptide-mapping protein identification. Regarding approximately 600–700 distinct protein spots detected on 2D gels, there has been found at least a two-fold change after exposure to low temperatures in about 10% of proteins in leaves and 13% of proteins in crowns. Protein and nitrogen metabolic processes have been influenced by low temperature to a similar extent in both tissues while catabolism, carbohydrate metabolism and proteins involved in stress response have been more affected in crowns than in leaves. The range of changes in protein abundance was generally higher in leaves and chloroplast proteins were frequently affected which suggests a priority to protect photosynthetic apparatus. Overall, our data proved existence of slightly different response strategies to low temperature stress in crowns and leaves, i.e., tissues with different biological role. Moreover, there have been found several proteins with large increase in accumulation, e.g., 33 kDa oxygen evolving protein of photosystem II in leaves and “enhanced disease susceptibility 1” in crowns; these proteins might have potential to indicate an enhanced level of frost tolerance in barley. |
format | Online Article Text |
id | pubmed-3645728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36457282013-05-13 Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor Hlaváčková, Iva Vítámvás, Pavel Šantrůček, Jiří Kosová, Klára Zelenková, Sylva Prášil, Ilja Tom Ovesná, Jaroslava Hynek, Radovan Kodíček, Milan Int J Mol Sci Article Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent an important environmental constraint limiting crop productivity. In this study changes in proteome of leaves and crowns in a frost tolerant winter barley cv. Luxor in relation to short and long term periods of cold followed by a brief frost treatment were studied in order to disclose proteins responsible for the cold hardening process in distinct plant tissues. The mentioned changes have been monitored using two dimensional difference gel electrophoresis (2D-DIGE) with subsequent peptide-mapping protein identification. Regarding approximately 600–700 distinct protein spots detected on 2D gels, there has been found at least a two-fold change after exposure to low temperatures in about 10% of proteins in leaves and 13% of proteins in crowns. Protein and nitrogen metabolic processes have been influenced by low temperature to a similar extent in both tissues while catabolism, carbohydrate metabolism and proteins involved in stress response have been more affected in crowns than in leaves. The range of changes in protein abundance was generally higher in leaves and chloroplast proteins were frequently affected which suggests a priority to protect photosynthetic apparatus. Overall, our data proved existence of slightly different response strategies to low temperature stress in crowns and leaves, i.e., tissues with different biological role. Moreover, there have been found several proteins with large increase in accumulation, e.g., 33 kDa oxygen evolving protein of photosystem II in leaves and “enhanced disease susceptibility 1” in crowns; these proteins might have potential to indicate an enhanced level of frost tolerance in barley. Molecular Diversity Preservation International (MDPI) 2013-04-12 /pmc/articles/PMC3645728/ /pubmed/23584021 http://dx.doi.org/10.3390/ijms14048000 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Hlaváčková, Iva Vítámvás, Pavel Šantrůček, Jiří Kosová, Klára Zelenková, Sylva Prášil, Ilja Tom Ovesná, Jaroslava Hynek, Radovan Kodíček, Milan Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor |
title | Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor |
title_full | Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor |
title_fullStr | Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor |
title_full_unstemmed | Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor |
title_short | Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor |
title_sort | proteins involved in distinct phases of cold hardening process in frost resistant winter barley (hordeum vulgare l.) cv luxor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645728/ https://www.ncbi.nlm.nih.gov/pubmed/23584021 http://dx.doi.org/10.3390/ijms14048000 |
work_keys_str_mv | AT hlavackovaiva proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor AT vitamvaspavel proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor AT santrucekjiri proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor AT kosovaklara proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor AT zelenkovasylva proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor AT prasililjatom proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor AT ovesnajaroslava proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor AT hynekradovan proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor AT kodicekmilan proteinsinvolvedindistinctphasesofcoldhardeningprocessinfrostresistantwinterbarleyhordeumvulgarelcvluxor |