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Biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of Himalayan region
Seven endophytic bacterial isolates were finally recovered from native sugarcane varieties at hilly areas namely Berinag, Champawat and Didihat of Uttarakhand state in northern Himalayan region. New isolates and two standard cultures—Azospirillum brasilense and Gluconacetobacter diazotrophicus, were...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646109/ https://www.ncbi.nlm.nih.gov/pubmed/28324369 http://dx.doi.org/10.1007/s13205-012-0084-2 |
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author | Singh, Digar Sharma, Anita Saini, Gurvinder Kaur |
author_facet | Singh, Digar Sharma, Anita Saini, Gurvinder Kaur |
author_sort | Singh, Digar |
collection | PubMed |
description | Seven endophytic bacterial isolates were finally recovered from native sugarcane varieties at hilly areas namely Berinag, Champawat and Didihat of Uttarakhand state in northern Himalayan region. New isolates and two standard cultures—Azospirillum brasilense and Gluconacetobacter diazotrophicus, were evaluated for their morphological, biochemical and molecular characteristics. Morphologically all were rod shaped, Gram-negative bacteria. Their plant growth promotory properties were also assessed which proved isolates RtBn and StBn as IAA producing. Except isolate StBn, all were phosphate solubilising and except RtBn all produced siderophores. Molecular characterisation of the isolates was performed using amplified 16S r-DNA restriction analysis. Similarity index in unweighted pair group method with arithmetic mean programme clustered the isolates according to their geographical distribution. Native isolates showed insignificant similarity with South American strains used as standards. nifH amplification was observed with all the isolates used in the study which again establish them as potential N-fixers. |
format | Online Article Text |
id | pubmed-3646109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-36461092013-05-07 Biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of Himalayan region Singh, Digar Sharma, Anita Saini, Gurvinder Kaur 3 Biotech Original Article Seven endophytic bacterial isolates were finally recovered from native sugarcane varieties at hilly areas namely Berinag, Champawat and Didihat of Uttarakhand state in northern Himalayan region. New isolates and two standard cultures—Azospirillum brasilense and Gluconacetobacter diazotrophicus, were evaluated for their morphological, biochemical and molecular characteristics. Morphologically all were rod shaped, Gram-negative bacteria. Their plant growth promotory properties were also assessed which proved isolates RtBn and StBn as IAA producing. Except isolate StBn, all were phosphate solubilising and except RtBn all produced siderophores. Molecular characterisation of the isolates was performed using amplified 16S r-DNA restriction analysis. Similarity index in unweighted pair group method with arithmetic mean programme clustered the isolates according to their geographical distribution. Native isolates showed insignificant similarity with South American strains used as standards. nifH amplification was observed with all the isolates used in the study which again establish them as potential N-fixers. Springer Berlin Heidelberg 2012-09-04 2013-06 /pmc/articles/PMC3646109/ /pubmed/28324369 http://dx.doi.org/10.1007/s13205-012-0084-2 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is published under license to BioMed Central Ltd. Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Article Singh, Digar Sharma, Anita Saini, Gurvinder Kaur Biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of Himalayan region |
title | Biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of Himalayan region |
title_full | Biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of Himalayan region |
title_fullStr | Biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of Himalayan region |
title_full_unstemmed | Biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of Himalayan region |
title_short | Biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of Himalayan region |
title_sort | biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of himalayan region |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646109/ https://www.ncbi.nlm.nih.gov/pubmed/28324369 http://dx.doi.org/10.1007/s13205-012-0084-2 |
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