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Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology

Melanins are predominantly indolic polymers which are extensively synthesized in animals, plants and microorganisms. It has wide applications in cosmetics, agriculture and medicine. In the present study, optimization of process parameters influencing melanin production was attempted using the respon...

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Autores principales: Surwase, Shripad N., Jadhav, Shekhar B., Phugare, Swapnil S., Jadhav, Jyoti P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646113/
https://www.ncbi.nlm.nih.gov/pubmed/28324367
http://dx.doi.org/10.1007/s13205-012-0082-4
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author Surwase, Shripad N.
Jadhav, Shekhar B.
Phugare, Swapnil S.
Jadhav, Jyoti P.
author_facet Surwase, Shripad N.
Jadhav, Shekhar B.
Phugare, Swapnil S.
Jadhav, Jyoti P.
author_sort Surwase, Shripad N.
collection PubMed
description Melanins are predominantly indolic polymers which are extensively synthesized in animals, plants and microorganisms. It has wide applications in cosmetics, agriculture and medicine. In the present study, optimization of process parameters influencing melanin production was attempted using the response surface methodology (RSM) from Brevundimonas sp. SGJ. A Plackett–Burman design was used for screening of critical components, while further optimization was carried out using the Box–Behnken design. The optimum conditions observed were pH 5.31, tryptone 1.440 g l(−1), l-tyrosine 1.872 g l(−1) and CuSO(4) 0.0366 g l(−1). Statistical analysis revealed that the model is significant with model F value 29.03 and R(2) value 0.9667. The optimization of process parameters using RSM resulted in a 3.05-fold increase in the yield of melanin. The intermittent addition of l-tyrosine enhanced the melanin yield to 6.811 g l(−1). The highest tyrosinase activity observed was 2,471 U mg(−1) at the 18th hour of the incubation period with dry cell weight of 0.711 g l(−1). The melanin production was confirmed by UV–Visible spectroscopy, FTIR and EPR analysis. Thus, Brevundimonas sp. SGJ has the potential to be a new source for the production of melanin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-012-0082-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-36461132013-05-07 Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology Surwase, Shripad N. Jadhav, Shekhar B. Phugare, Swapnil S. Jadhav, Jyoti P. 3 Biotech Original Article Melanins are predominantly indolic polymers which are extensively synthesized in animals, plants and microorganisms. It has wide applications in cosmetics, agriculture and medicine. In the present study, optimization of process parameters influencing melanin production was attempted using the response surface methodology (RSM) from Brevundimonas sp. SGJ. A Plackett–Burman design was used for screening of critical components, while further optimization was carried out using the Box–Behnken design. The optimum conditions observed were pH 5.31, tryptone 1.440 g l(−1), l-tyrosine 1.872 g l(−1) and CuSO(4) 0.0366 g l(−1). Statistical analysis revealed that the model is significant with model F value 29.03 and R(2) value 0.9667. The optimization of process parameters using RSM resulted in a 3.05-fold increase in the yield of melanin. The intermittent addition of l-tyrosine enhanced the melanin yield to 6.811 g l(−1). The highest tyrosinase activity observed was 2,471 U mg(−1) at the 18th hour of the incubation period with dry cell weight of 0.711 g l(−1). The melanin production was confirmed by UV–Visible spectroscopy, FTIR and EPR analysis. Thus, Brevundimonas sp. SGJ has the potential to be a new source for the production of melanin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-012-0082-4) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2012-08-07 2013-06 /pmc/articles/PMC3646113/ /pubmed/28324367 http://dx.doi.org/10.1007/s13205-012-0082-4 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is published under license to BioMed Central Ltd. Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Surwase, Shripad N.
Jadhav, Shekhar B.
Phugare, Swapnil S.
Jadhav, Jyoti P.
Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology
title Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology
title_full Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology
title_fullStr Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology
title_full_unstemmed Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology
title_short Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology
title_sort optimization of melanin production by brevundimonas sp. sgj using response surface methodology
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646113/
https://www.ncbi.nlm.nih.gov/pubmed/28324367
http://dx.doi.org/10.1007/s13205-012-0082-4
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