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Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3(+) dendritic cells at cross presentation
Human BDCA3(+) dendritic cells (DCs), the proposed equivalent to mouse CD8α(+) DCs, are widely thought to cross present antigens on MHC class I (MHCI) molecules more efficiently than other DC populations. If true, it is unclear whether this reflects specialization for cross presentation or a general...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646496/ https://www.ncbi.nlm.nih.gov/pubmed/23569326 http://dx.doi.org/10.1084/jem.20121251 |
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author | Cohn, Lillian Chatterjee, Bithi Esselborn, Filipp Smed-Sörensen, Anna Nakamura, Norihiro Chalouni, Cécile Lee, Byoung-Chul Vandlen, Richard Keler, Tibor Lauer, Peter Brockstedt, Dirk Mellman, Ira Delamarre, Lélia |
author_facet | Cohn, Lillian Chatterjee, Bithi Esselborn, Filipp Smed-Sörensen, Anna Nakamura, Norihiro Chalouni, Cécile Lee, Byoung-Chul Vandlen, Richard Keler, Tibor Lauer, Peter Brockstedt, Dirk Mellman, Ira Delamarre, Lélia |
author_sort | Cohn, Lillian |
collection | PubMed |
description | Human BDCA3(+) dendritic cells (DCs), the proposed equivalent to mouse CD8α(+) DCs, are widely thought to cross present antigens on MHC class I (MHCI) molecules more efficiently than other DC populations. If true, it is unclear whether this reflects specialization for cross presentation or a generally enhanced ability to present antigens on MHCI. We compared presentation by BDCA3(+) DCs with BDCA1(+) DCs using a quantitative approach whereby antigens were targeted to distinct intracellular compartments by receptor-mediated internalization. As expected, BDCA3(+) DCs were superior at cross presentation of antigens delivered to late endosomes and lysosomes by uptake of anti-DEC205 antibody conjugated to antigen. This difference may reflect a greater efficiency of antigen escape from BDCA3(+) DC lysosomes. In contrast, if antigens were delivered to early endosomes through CD40 or CD11c, BDCA1(+) DCs were as efficient at cross presentation as BDCA3(+) DCs. Because BDCA3(+) DCs and BDCA1(+) DCs were also equivalent at presenting peptides and endogenously synthesized antigens, BDCA3(+) DCs are not likely to possess mechanisms for cross presentation that are specific to this subset. Thus, multiple DC populations may be comparably effective at presenting exogenous antigens to CD8(+) T cells as long as the antigen is delivered to early endocytic compartments. |
format | Online Article Text |
id | pubmed-3646496 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36464962013-11-06 Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3(+) dendritic cells at cross presentation Cohn, Lillian Chatterjee, Bithi Esselborn, Filipp Smed-Sörensen, Anna Nakamura, Norihiro Chalouni, Cécile Lee, Byoung-Chul Vandlen, Richard Keler, Tibor Lauer, Peter Brockstedt, Dirk Mellman, Ira Delamarre, Lélia J Exp Med Article Human BDCA3(+) dendritic cells (DCs), the proposed equivalent to mouse CD8α(+) DCs, are widely thought to cross present antigens on MHC class I (MHCI) molecules more efficiently than other DC populations. If true, it is unclear whether this reflects specialization for cross presentation or a generally enhanced ability to present antigens on MHCI. We compared presentation by BDCA3(+) DCs with BDCA1(+) DCs using a quantitative approach whereby antigens were targeted to distinct intracellular compartments by receptor-mediated internalization. As expected, BDCA3(+) DCs were superior at cross presentation of antigens delivered to late endosomes and lysosomes by uptake of anti-DEC205 antibody conjugated to antigen. This difference may reflect a greater efficiency of antigen escape from BDCA3(+) DC lysosomes. In contrast, if antigens were delivered to early endosomes through CD40 or CD11c, BDCA1(+) DCs were as efficient at cross presentation as BDCA3(+) DCs. Because BDCA3(+) DCs and BDCA1(+) DCs were also equivalent at presenting peptides and endogenously synthesized antigens, BDCA3(+) DCs are not likely to possess mechanisms for cross presentation that are specific to this subset. Thus, multiple DC populations may be comparably effective at presenting exogenous antigens to CD8(+) T cells as long as the antigen is delivered to early endocytic compartments. The Rockefeller University Press 2013-05-06 /pmc/articles/PMC3646496/ /pubmed/23569326 http://dx.doi.org/10.1084/jem.20121251 Text en © 2013 Cohn et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Article Cohn, Lillian Chatterjee, Bithi Esselborn, Filipp Smed-Sörensen, Anna Nakamura, Norihiro Chalouni, Cécile Lee, Byoung-Chul Vandlen, Richard Keler, Tibor Lauer, Peter Brockstedt, Dirk Mellman, Ira Delamarre, Lélia Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3(+) dendritic cells at cross presentation |
title | Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3(+) dendritic cells at cross presentation |
title_full | Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3(+) dendritic cells at cross presentation |
title_fullStr | Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3(+) dendritic cells at cross presentation |
title_full_unstemmed | Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3(+) dendritic cells at cross presentation |
title_short | Antigen delivery to early endosomes eliminates the superiority of human blood BDCA3(+) dendritic cells at cross presentation |
title_sort | antigen delivery to early endosomes eliminates the superiority of human blood bdca3(+) dendritic cells at cross presentation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646496/ https://www.ncbi.nlm.nih.gov/pubmed/23569326 http://dx.doi.org/10.1084/jem.20121251 |
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