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Three-photon excited fluorescence imaging of unstained tissue using a GRIN lens endoscope

We present a compact and portable three-photon gradient index (GRIN) lens endoscope system suitable for imaging of unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The lateral and axial resolution in water is 1.0 μm and 9.5 μm, respectiv...

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Detalles Bibliográficos
Autores principales: Huland, David M., Charan, Kriti, Ouzounov, Dimitre G., Jones, Jason S., Nishimura, Nozomi, Xu, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646593/
https://www.ncbi.nlm.nih.gov/pubmed/23667782
http://dx.doi.org/10.1364/BOE.4.000652
Descripción
Sumario:We present a compact and portable three-photon gradient index (GRIN) lens endoscope system suitable for imaging of unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The lateral and axial resolution in water is 1.0 μm and 9.5 μm, respectively. The ~200 μm diameter field of view is imaged at 2 frames/s using a fiber-based excitation source at 1040 nm. Ex vivo imaging is demonstrated with unstained mouse lung at 5.9 mW average power. These results demonstrate the feasibility of three-photon GRIN lens endoscopy for optical biopsy.